High-throughput bacterial genome sequencing: An embarrassment of choice, a world of opportunity

Nicholas J. Loman; Chrystala Constantinidou; Jacqueline Z.M. Chan; Mihail Halachev; Martin Sergeant; Charles W. Penn; Esther R. Robinson; Mark J. Pallen

doi:10.1038/nrmicro2850

High-throughput bacterial genome sequencing: An embarrassment of choice, a world of opportunity

Nicholas J. Loman
, Chrystala Constantinidou
, Jacqueline Z.M. Chan
, Mihail Halachev
, Martin Sergeant
, Charles W. Penn
, Esther R. Robinson
, Mark J. Pallen^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

349 Citations (Scopus)

Abstract

Here, we take a snapshot of the high-throughput sequencing platforms, together with the relevant analytical tools, that are available to microbiologists in 2012, and evaluate the strengths and weaknesses of these platforms in obtaining bacterial genome sequences. We also scan the horizon of future possibilities, speculating on how the availability of sequencing that is 'too cheap to metre' might change the face of microbiology forever.

Original language	English
Pages (from-to)	599-606
Number of pages	8
Journal	Nature Reviews Microbiology
Volume	10
Issue number	9
DOIs	https://doi.org/10.1038/nrmicro2850
Publication status	Published - Sept 2012
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Access to Document

10.1038/nrmicro2850

Cite this

@article{649f971dbb0b4b23baea278252e48019,

title = "High-throughput bacterial genome sequencing: An embarrassment of choice, a world of opportunity",

abstract = "Here, we take a snapshot of the high-throughput sequencing platforms, together with the relevant analytical tools, that are available to microbiologists in 2012, and evaluate the strengths and weaknesses of these platforms in obtaining bacterial genome sequences. We also scan the horizon of future possibilities, speculating on how the availability of sequencing that is 'too cheap to metre' might change the face of microbiology forever.",

author = "Loman, \{Nicholas J.\} and Chrystala Constantinidou and Chan, \{Jacqueline Z.M.\} and Mihail Halachev and Martin Sergeant and Penn, \{Charles W.\} and Robinson, \{Esther R.\} and Pallen, \{Mark J.\}",

year = "2012",

month = sep,

doi = "10.1038/nrmicro2850",

language = "English",

volume = "10",

pages = "599--606",

journal = "Nature Reviews Microbiology",

issn = "1740-1526",

publisher = "Nature Research",

number = "9",

}

TY - JOUR

T1 - High-throughput bacterial genome sequencing

T2 - An embarrassment of choice, a world of opportunity

AU - Loman, Nicholas J.

AU - Constantinidou, Chrystala

AU - Chan, Jacqueline Z.M.

AU - Halachev, Mihail

AU - Sergeant, Martin

AU - Penn, Charles W.

AU - Robinson, Esther R.

AU - Pallen, Mark J.

PY - 2012/9

Y1 - 2012/9

N2 - Here, we take a snapshot of the high-throughput sequencing platforms, together with the relevant analytical tools, that are available to microbiologists in 2012, and evaluate the strengths and weaknesses of these platforms in obtaining bacterial genome sequences. We also scan the horizon of future possibilities, speculating on how the availability of sequencing that is 'too cheap to metre' might change the face of microbiology forever.

AB - Here, we take a snapshot of the high-throughput sequencing platforms, together with the relevant analytical tools, that are available to microbiologists in 2012, and evaluate the strengths and weaknesses of these platforms in obtaining bacterial genome sequences. We also scan the horizon of future possibilities, speculating on how the availability of sequencing that is 'too cheap to metre' might change the face of microbiology forever.

UR - https://www.scopus.com/pages/publications/84865179264

U2 - 10.1038/nrmicro2850

DO - 10.1038/nrmicro2850

M3 - Article

C2 - 22864262

AN - SCOPUS:84865179264

SN - 1740-1526

VL - 10

SP - 599

EP - 606

JO - Nature Reviews Microbiology

JF - Nature Reviews Microbiology

IS - 9

ER -

High-throughput bacterial genome sequencing: An embarrassment of choice, a world of opportunity

Abstract

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this