High interlaboratory reproducibility of DNA sequence-based typing of bacteria in a multicenter study

M. Aires-De-Sousa; K. Boye; H. De Lencastre; A. Deplano; M. C. Enright; J. Etienne; A. Friedrich; D. Harmsen; A. Holmes; X. W. Huijsdens; Angela Kearns; A. Mellmann; H. Meugnier; J. K. Rasheed; E. Spalburg; B. Strommenger; M. J. Struelens; F. C. Tenover; J. Thomas; U. Vogel; H. Westh; J. Xu; W. Witte

doi:10.1128/JCM.44.2.619-621.2006

High interlaboratory reproducibility of DNA sequence-based typing of bacteria in a multicenter study

M. Aires-De-Sousa
, K. Boye
, H. De Lencastre
, A. Deplano
, M. C. Enright
, J. Etienne
, A. Friedrich
, D. Harmsen^*
, A. Holmes
, X. W. Huijsdens
, Angela Kearns
, A. Mellmann
, H. Meugnier
, J. K. Rasheed
, E. Spalburg
, B. Strommenger
, M. J. Struelens
, F. C. Tenover
, J. Thomas
, U. Vogel

H. Westh, J. Xu, W. Witte

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

193 Citations (Scopus)

Abstract

Current DNA amplification-based typing methods for bacterial pathogens often lack interlaboratory reproducibility. In this international study, DNA sequence-based typing of the Staphylococcus aureus protein A gene (spa, 110 to 422 bp) showed 100% intra- and interlaboratory reproducibility without extensive harmonization of protocols for 30 blind-coded S. aureus DNA samples sent to 10 laboratories. Specialized software for automated sequence analysis ensured a common typing nomenclature.

Original language	English
Pages (from-to)	619-621
Number of pages	3
Journal	Journal of Clinical Microbiology
Volume	44
Issue number	2
DOIs	https://doi.org/10.1128/JCM.44.2.619-621.2006
Publication status	Published - Feb 2006

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10.1128/JCM.44.2.619-621.2006

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Aires-De-Sousa, M., Boye, K., De Lencastre, H., Deplano, A., Enright, M. C., Etienne, J., Friedrich, A., Harmsen, D., Holmes, A., Huijsdens, X. W., Kearns, A., Mellmann, A., Meugnier, H., Rasheed, J. K., Spalburg, E., Strommenger, B., Struelens, M. J., Tenover, F. C., Thomas, J., ... Witte, W. (2006). High interlaboratory reproducibility of DNA sequence-based typing of bacteria in a multicenter study. Journal of Clinical Microbiology, 44(2), 619-621. https://doi.org/10.1128/JCM.44.2.619-621.2006

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title = "High interlaboratory reproducibility of DNA sequence-based typing of bacteria in a multicenter study",

abstract = "Current DNA amplification-based typing methods for bacterial pathogens often lack interlaboratory reproducibility. In this international study, DNA sequence-based typing of the Staphylococcus aureus protein A gene (spa, 110 to 422 bp) showed 100\% intra- and interlaboratory reproducibility without extensive harmonization of protocols for 30 blind-coded S. aureus DNA samples sent to 10 laboratories. Specialized software for automated sequence analysis ensured a common typing nomenclature.",

author = "M. Aires-De-Sousa and K. Boye and \{De Lencastre\}, H. and A. Deplano and Enright, \{M. C.\} and J. Etienne and A. Friedrich and D. Harmsen and A. Holmes and Huijsdens, \{X. W.\} and Angela Kearns and A. Mellmann and H. Meugnier and Rasheed, \{J. K.\} and E. Spalburg and B. Strommenger and Struelens, \{M. J.\} and Tenover, \{F. C.\} and J. Thomas and U. Vogel and H. Westh and J. Xu and W. Witte",

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doi = "10.1128/JCM.44.2.619-621.2006",

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Aires-De-Sousa, M, Boye, K, De Lencastre, H, Deplano, A, Enright, MC, Etienne, J, Friedrich, A, Harmsen, D, Holmes, A, Huijsdens, XW, Kearns, A, Mellmann, A, Meugnier, H, Rasheed, JK, Spalburg, E, Strommenger, B, Struelens, MJ, Tenover, FC, Thomas, J, Vogel, U, Westh, H, Xu, J & Witte, W 2006, 'High interlaboratory reproducibility of DNA sequence-based typing of bacteria in a multicenter study', Journal of Clinical Microbiology, vol. 44, no. 2, pp. 619-621. https://doi.org/10.1128/JCM.44.2.619-621.2006

TY - JOUR

T1 - High interlaboratory reproducibility of DNA sequence-based typing of bacteria in a multicenter study

AU - Aires-De-Sousa, M.

AU - Boye, K.

AU - De Lencastre, H.

AU - Deplano, A.

AU - Enright, M. C.

AU - Etienne, J.

AU - Friedrich, A.

AU - Harmsen, D.

AU - Holmes, A.

AU - Huijsdens, X. W.

AU - Kearns, Angela

AU - Mellmann, A.

AU - Meugnier, H.

AU - Rasheed, J. K.

AU - Spalburg, E.

AU - Strommenger, B.

AU - Struelens, M. J.

AU - Tenover, F. C.

AU - Thomas, J.

AU - Vogel, U.

AU - Westh, H.

AU - Xu, J.

AU - Witte, W.

PY - 2006/2

Y1 - 2006/2

N2 - Current DNA amplification-based typing methods for bacterial pathogens often lack interlaboratory reproducibility. In this international study, DNA sequence-based typing of the Staphylococcus aureus protein A gene (spa, 110 to 422 bp) showed 100% intra- and interlaboratory reproducibility without extensive harmonization of protocols for 30 blind-coded S. aureus DNA samples sent to 10 laboratories. Specialized software for automated sequence analysis ensured a common typing nomenclature.

AB - Current DNA amplification-based typing methods for bacterial pathogens often lack interlaboratory reproducibility. In this international study, DNA sequence-based typing of the Staphylococcus aureus protein A gene (spa, 110 to 422 bp) showed 100% intra- and interlaboratory reproducibility without extensive harmonization of protocols for 30 blind-coded S. aureus DNA samples sent to 10 laboratories. Specialized software for automated sequence analysis ensured a common typing nomenclature.

UR - https://www.scopus.com/pages/publications/32344441986

U2 - 10.1128/JCM.44.2.619-621.2006

DO - 10.1128/JCM.44.2.619-621.2006

M3 - Article

C2 - 16455927

AN - SCOPUS:32344441986

SN - 0095-1137

VL - 44

SP - 619

EP - 621

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 2

ER -

High interlaboratory reproducibility of DNA sequence-based typing of bacteria in a multicenter study

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