TY - JOUR
T1 - High CSF transforming growth factor b levels after subarachnoid haemorrhage
T2 - Association with chronic communicating hydrocephalus
AU - Douglas, M. R.
AU - Daniel, M.
AU - Lagord, C.
AU - Akinwunmi, J.
AU - Jackowski, A.
AU - Cooper, C.
AU - Berry, M.
AU - Logan, A.
PY - 2009/5
Y1 - 2009/5
N2 - Background: Chronic communicating hydrocephalus is a common sequela of subarachnoid haemorrhage and develops when the flow and drainage of CSF are impaired after fibrosis in the subarachnoid space. Released by platelets into the CSF after subarachnoid haemorrhage, transforming growth factor (TGF)b1/b2 are potent fibrogenic agents that may promote post-haemorrhagic fibrosis and chronic communicating hydrocephalus. Methods: Temporal changes in total (latent plus active) TGFb1/b2 CSF levels of post-haemorrhage patients developing acute hydrocephalus were measured using ELISA to discover if titres were higher in patients that subsequently developed chronic communicating hydrocephalus, compared with those that did not. Results: Mean (SD) CSF levels of total TGFb1 were 97 (42) pg/ml and total TGFb2 were 395 (39) pg/ml in control patients with (non-haemorrhagic) hydrocephalus. For days 1-5 post-subarachnoid haemorrhage (dph), levels of 1427 (242) pg/ml and 976 (191) pg/ml were seen for total TGFb1 and TGFb2, respectively. Beyond 5 dph, total TGFb1/b2 levels declined but remained significantly elevated (p,0.01) above control patient values for at least 19 dph. Haemorrhagic patients that went on to develop chronic communicating hydrocephalus had significantly higher levels of total TGFb1 (p,0.01) and TGFb2 (p,0.05) between 1 and 9 dph, compared with those of haemorrhagic patients that did not. Conclusions: Acutely measured levels of TGFb1/b2 in the CSF of patients with subarachnoid haemorrhage are thus potential prognostic biomarkers for the subsequent development of chronic communicating hydrocephalus, indicating likely dependency on CSF shunting.
AB - Background: Chronic communicating hydrocephalus is a common sequela of subarachnoid haemorrhage and develops when the flow and drainage of CSF are impaired after fibrosis in the subarachnoid space. Released by platelets into the CSF after subarachnoid haemorrhage, transforming growth factor (TGF)b1/b2 are potent fibrogenic agents that may promote post-haemorrhagic fibrosis and chronic communicating hydrocephalus. Methods: Temporal changes in total (latent plus active) TGFb1/b2 CSF levels of post-haemorrhage patients developing acute hydrocephalus were measured using ELISA to discover if titres were higher in patients that subsequently developed chronic communicating hydrocephalus, compared with those that did not. Results: Mean (SD) CSF levels of total TGFb1 were 97 (42) pg/ml and total TGFb2 were 395 (39) pg/ml in control patients with (non-haemorrhagic) hydrocephalus. For days 1-5 post-subarachnoid haemorrhage (dph), levels of 1427 (242) pg/ml and 976 (191) pg/ml were seen for total TGFb1 and TGFb2, respectively. Beyond 5 dph, total TGFb1/b2 levels declined but remained significantly elevated (p,0.01) above control patient values for at least 19 dph. Haemorrhagic patients that went on to develop chronic communicating hydrocephalus had significantly higher levels of total TGFb1 (p,0.01) and TGFb2 (p,0.05) between 1 and 9 dph, compared with those of haemorrhagic patients that did not. Conclusions: Acutely measured levels of TGFb1/b2 in the CSF of patients with subarachnoid haemorrhage are thus potential prognostic biomarkers for the subsequent development of chronic communicating hydrocephalus, indicating likely dependency on CSF shunting.
UR - http://www.scopus.com/inward/record.url?scp=65449137153&partnerID=8YFLogxK
U2 - 10.1136/jnnp.2008.155671
DO - 10.1136/jnnp.2008.155671
M3 - Article
C2 - 19066194
AN - SCOPUS:65449137153
SN - 0022-3050
VL - 80
SP - 545
EP - 550
JO - Journal of Neurology, Neurosurgery and Psychiatry
JF - Journal of Neurology, Neurosurgery and Psychiatry
IS - 5
ER -
