TY - JOUR
T1 - High clonal heterogeneity of Panton-Valentine leukocidin-positive meticillin-resistant Staphylococcus aureus strains from skin and soft-tissue infections in the Province of Bolzano, Northern Italy
AU - Aschbacher, Richard
AU - Pichon, Bruno
AU - Spoladore, Greta
AU - Pagani, Elisabetta
AU - Innocenti, Patrizia
AU - Moroder, Ludwig
AU - Ganner, Mark
AU - Hill, Robert
AU - Pike, Rachel
AU - Ganthaler, Oswald
AU - Pagani, Leonardo
AU - Larcher, Clara
AU - Kearns, Angela
PY - 2012/6
Y1 - 2012/6
N2 - Panton-Valentine leukocidin (PVL)-positive community-associated meticillin-resistant Staphylococcus aureus (CA-MRSA) isolates are widespread in many countries, with varying distribution and epidemiology. The aim of this study was to characterise 10 PVL-positive MRSA isolates collected during February 2010 to January 2011 from skin and soft-tissue infections in the North Italian Province of Bolzano. Accessory gene regulator (agr) typing, staphylococcal cassette chromosome mec (SCCmec) typing, staphylococcal protein A (spa) gene typing, multilocus sequence typing, toxin gene profiling, polymerase chain reaction for type I arginine catabolic mobile element (ACME) and antimicrobial resistance typing were applied to the isolates. Eight different CA-MRSA clones were identified, including ST30-IVc, ST772-V, ST80-IVc, ST5-IVc, ST88-IVa, ST93-IVa, ST8-IVc and the type I ACME-positive ST8-IVa. The high heterogeneity of PVL-positive MRSA probably reflects the introduction of different clones by international travellers or immigrants.
AB - Panton-Valentine leukocidin (PVL)-positive community-associated meticillin-resistant Staphylococcus aureus (CA-MRSA) isolates are widespread in many countries, with varying distribution and epidemiology. The aim of this study was to characterise 10 PVL-positive MRSA isolates collected during February 2010 to January 2011 from skin and soft-tissue infections in the North Italian Province of Bolzano. Accessory gene regulator (agr) typing, staphylococcal cassette chromosome mec (SCCmec) typing, staphylococcal protein A (spa) gene typing, multilocus sequence typing, toxin gene profiling, polymerase chain reaction for type I arginine catabolic mobile element (ACME) and antimicrobial resistance typing were applied to the isolates. Eight different CA-MRSA clones were identified, including ST30-IVc, ST772-V, ST80-IVc, ST5-IVc, ST88-IVa, ST93-IVa, ST8-IVc and the type I ACME-positive ST8-IVa. The high heterogeneity of PVL-positive MRSA probably reflects the introduction of different clones by international travellers or immigrants.
KW - ACME
KW - Arginine catabolic mobile element
KW - CA-MRSA
KW - Community-associated meticillin-resistant Staphylococcus aureus
KW - PVL
KW - Panton-Valentine leukocidin
KW - SCCmec
KW - ST93
UR - http://www.scopus.com/inward/record.url?scp=84860474580&partnerID=8YFLogxK
U2 - 10.1016/j.ijantimicag.2012.02.004
DO - 10.1016/j.ijantimicag.2012.02.004
M3 - Article
C2 - 22481056
AN - SCOPUS:84860474580
SN - 0924-8579
VL - 39
SP - 522
EP - 525
JO - International Journal of Antimicrobial Agents
JF - International Journal of Antimicrobial Agents
IS - 6
ER -
