Haemin-restriction influences haemin-binding haemagglutination and protease activity of cells and extracellular membrane vesicles of Porphyromonas gingivalis W50

J. W. Smalley*, A. J. Birss, A. S. McKee, Phillip Marsh

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

39 Citations (Scopus)

Abstract

Porphyromonas gingivalis strain W50 was grown in a chemostat either under haemin limitation or haemin excess at pH 7.3. Cells and the extracellular vesicle (ECV) and extracellular protein (EP) fractions were separated, quantified, and assayed for haemagglutination, protease activity and haemin binding. Under haemin-limitation, despite a reduction in cell yield, there was a 2.5-fold increase in the gravimetric yield of extracellular vesicles. Cells and vesicles from haemin-limited cultures, haemagglutinated sheep red blood cells to higher titres than their haemin-excess counterparts. Growth in haemin-excess conditions resulted in increased haemin-binding capacities of ECV, cells and EDTA-extracted outer membrane. Cells grown under haemin-excess showed a 2-fold elevation in specific activity towards the substrate N-α-benzoyl-l-arginine-p-nitroanilide (l-BAPNA) compared to haemin-limited cells. The specific activities against l-BAPNA for haemin-limited ECV were 3-fold greater than their haemin-excess counterparts. These vesicle activities represented 25% and 3% of the total culture protease activity under haemin limited and haemin excess conditions respectively.

Original languageEnglish
Pages (from-to)63-67
Number of pages5
JournalFEMS Microbiology Letters
Volume90
Issue number1
DOIs
Publication statusPublished - 15 Dec 1991

Keywords

  • Extracellular vesicles
  • Haemin
  • Porphyromonas gingivalis
  • Protease
  • Virulence

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