Guidelines for the use of cell lines in biomedical research

R. J. Geraghty*, A. Capes-Davis, J. M. Davis, J. Downward, R. I. Freshney, I. Knezevic, R. Lovell-Badge, J. R.W. Masters, J. Meredith, G. N. Stacey, P. Thraves, M. Vias

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

196 Citations (Scopus)

Abstract

Cell-line misidentification and contamination with microorganisms, such as mycoplasma, together with instability, both genetic and phenotypic, are among the problems that continue to affect cell culture. Many of these problems are avoidable with the necessary foresight, and these Guidelines have been prepared to provide those new to the field and others engaged in teaching and instruction with the information necessary to increase their awareness of the problems and to enable them to deal with them effectively. The Guidelines cover areas such as development, acquisition, authentication, cryopreservation, transfer of cell lines between laboratories, microbial contamination, characterisation, instability and misidentification. Advice is also given on complying with current legal and ethical requirements when deriving cell lines from human and animal tissues, the selection and maintenance of equipment and how to deal with problems that may arise.

Original languageEnglish
Pages (from-to)1021-1046
Number of pages26
JournalBritish Journal of Cancer
Volume111
Issue number6
DOIs
Publication statusPublished - 9 Sep 2014

Bibliographical note

Publisher Copyright:
© 2014 Cancer Research UK. All rights reserved.

Keywords

  • cell culture
  • cell line
  • cell line misidentification
  • cryostorage
  • Human Fertilisation and Embryology Act
  • human tissue
  • Human Tissue Act
  • Human Tissue Authority
  • mycoplasma contamination
  • STR profiling

Fingerprint

Dive into the research topics of 'Guidelines for the use of cell lines in biomedical research'. Together they form a unique fingerprint.

Cite this