Genotypic and phenotypic characteristics of Corynebacterium diphtheriae strains isolated from patients in Belarus during an epidemic period

Leonid Titov*, Valentina Kolodkina, Alina Dronina, Francine Grimont, Patrick A.D. Grimont, Monique Lejay-Collin, Aruni De Zoysa, Constantin Andronescu, Angela Diaconescu, Byanca Marin, Androulla Efstratiou

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    12 Citations (Scopus)

    Abstract

    One hundred two Corynebacterium diphtheriae strains (93 of the gravis biotype and nine of the mitis biotype) isolated from clinical cases during the Belarus diphtheria epidemic were characterized by biotyping, toxigenicity testing by the Elek test and an indirect hemagglutination assay, phage typing, and ribotyping. The gravis biotype strains were characterized as high and medium toxin producers, and strains of biotype mitis were characterized as low and medium toxin producers. Most strains (82 of 102) were distributed among five phage types. Seventy-two strains (64 of the gravis biotype and 8 of the mitis biotype) belonged to phage type VI 1s5,34add. Hybridization of genomic DNA digested with BstEII and PvuII revealed five ribotype patterns, namely, D1, D4, D6, D7, and D13. The majority of gravis biotype strains belonged to ribotypes D1 (49 of 93) and D4 (33 of 93) and included one clonal group of C. diphtheriae. This clone predominated in all regions in Belarus. There was a statistical association between ribotypes and phage types but not between ribotypes and levels of toxin production.

    Original languageEnglish
    Pages (from-to)1285-1288
    Number of pages4
    JournalJournal of Clinical Microbiology
    Volume41
    Issue number3
    DOIs
    Publication statusPublished - 1 Mar 2003

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