Genomic surveillance detects Salmonella enterica serovar Paratyphi A harbouring blaCTX-M-15 from a traveller returning from Bangladesh

Satheesh Nair*, Martin Day, Gauri Godbole, Tranprit Saluja, Gemma C. Langridge, Timothy J. Dallman, Marie Chattaway

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)
13 Downloads (Pure)

Abstract

Whole genome sequencing (WGS) has been used routinely by Public Health England (PHE) for identification, surveillance and monitoring of resistance determinants in referred Salmonella isolates since 2015. We report the first identified case of extended-spectrum-βlactamase (ESBL) Salmonella enterica serovar Paratyphi A (S. Paratyphi A) isolated from a traveller returning to England from Bangladesh in November 2017. The isolate (440915) was resistant to ciprofloxacin and harboured both the mobile element ISEcp9 –blaCTX-M-15hp-tnpA and blaTEM-191, associated with ESBL production. Phenotypic resistance was subsequently confirmed by Antimicrobial Susceptibility Testing (AST). S. Paratyphi A 440915 harboured an IncI1 plasmid previously reported to encode ESBL elements in Enterobacteriaceae and recently described in a S. Typhi isolate from Bangladesh. Results from this study indicate the importance of monitoring imported drug resistance for typhoidal salmonellae as ceftriaxone is the first line antibiotic treatment for complicated enteric fever in England. We conclude that WGS provides a rapid, accurate method for surveillance of drug resistance genes in Salmonella, leading to the first reported case of ESBL producing S. Paratyphi A and continues to inform the national treatment guidelines for management of enteric fever.

Original languageEnglish
Article numbere0228250
JournalPLoS ONE
Volume15
Issue number1
DOIs
Publication statusPublished - 30 Jan 2020

Bibliographical note

Funding Information: GL was supported by the BBSRC Institute Strategic Programme Microbes in the Food Chain BB/R012504/1.

Open Access: This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Publisher Copyright: © 2020 Nair et al.

Citation: Nair S, Day M, Godbole G, Saluja T, Langridge GC, Dallman TJ, et al. (2020) Genomic surveillance detects Salmonella enterica serovar Paratyphi A harbouring blaCTX-M-15 from a traveller returning from Bangladesh. PLoS ONE 15(1): e0228250.

DOI: https://doi.org/10.1371/journal.pone.0228250

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