Genomic and Epidemiological Evidence of a Dominant Panton-Valentine Leucocidin-Positive Methicillin Resistant Staphylococcus aureus Lineage in Sri Lanka and Presence among Isolates from the United Kingdom and Australia

Sharla M. McTavish; Sarah J. Snow; Ellie C. Cook; Bruno Pichon; Sarah Coleman; Geoffrey W. Coombs; Stanley Pang; Cesar A. Arias; Lorena Díaz; Emma Boldock; Steve Davies; Mangala Udukala; Angela Kearns; Sisira Siribaddana; Thushan I. De Silva

doi:10.3389/fcimb.2019.00123

Genomic and Epidemiological Evidence of a Dominant Panton-Valentine Leucocidin-Positive Methicillin Resistant Staphylococcus aureus Lineage in Sri Lanka and Presence among Isolates from the United Kingdom and Australia

Sharla M. McTavish, Sarah J. Snow, Ellie C. Cook, Bruno Pichon, Sarah Coleman, Geoffrey W. Coombs, Stanley Pang, Cesar A. Arias, Lorena Díaz, Emma Boldock, Steve Davies, Mangala Udukala, Angela Kearns, Sisira Siribaddana, Thushan I. De Silva^*

^*Corresponding author for this work

AMR & HCAI Divisional Office

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

Objective: To undertake the first detailed genomic analysis of methicillin-resistant Staphylococcus aureus (MRSA) isolated in Sri Lanka. Methods: A prospective observational study was performed on 94 MRSA isolates collected over a 4 months period from the Anuradhapura Teaching Hospital, Sri Lanka. Screening for mecA, mecC, and the Panton-Valentine leucocidin (PVL)-associated lukS-PV/lukF-PV genes and molecular characterization by spa typing was undertaken. Whole genome sequencing (WGS) and phylogenetic analysis was performed on selected multilocus sequence type (MLST) clonal complex 5 (CC5) isolates from Sri Lanka, England, Australia, and Argentina. Results: All 94 MRSA harbored the mecA gene. Nineteen spa types belonging to nine MLST clonal complexes were identified. Where origin of the sample was recorded, most isolates were from skin and soft tissue infections (70/91; 76.9%), with fewer causing bacteremia (16/91; 17.6%), empyema (3/91; 3.3%) and osteomyelitis (2/91; 2.2%). Sixty two (65.9%) isolates were PVL positive with the majority (56 isolates; 90.3%) belonging to a dominant CC5 lineage. This lineage, PVL-positive ST5-MRSA-IVc, was associated with both community and hospital-onset infections. Based on WGS, representative PVL-positive ST5-MRSA-IVc isolates from Sri Lanka, England and Australia formed a single phylogenetic clade, suggesting wide geographical circulation. Conclusions: We present the most detailed genomic analysis of MRSA isolated in Sri Lanka to date. The analysis identified a PVL-positive ST5-MRSA-IVc that is prevalent among MRSA causing clinical infections in Sri Lanka. Furthermore, this clone was also found among isolates from the United Kingdom and Australia.

Original language	English
Article number	123
Journal	Frontiers in Cellular and Infection Microbiology
Volume	9
Issue number	APR
DOIs	https://doi.org/10.3389/fcimb.2019.00123
Publication status	Published - 2019

Bibliographical note

Funding Information:
TdS is funded by a Wellcome Trust Intermediate Clinical Fellowship (110058/Z/15/Z). LD was supported by grants COL130871250417 and COL130874455850 from Colciencias. CA is funded through an NIH-NIAID grant K24 AI-AI121296, R01-AI134637, R01-AI093749 and R21 AI121519. During fieldwork, SJS and EC were supported by student grants from The Sheffield Medico-Chirurgical Society, The Sheffield Grammer School’ Trust and The 800th Lord Mayor’s Anniversary Trust.

Publisher Copyright:
Copyright © 2019 McTavish, Snow, Cook, Pichon, Coleman, Coombs, Pang, Arias, Díaz, Boldock, Davies, Udukala, Kearns, Siribaddana and de Silva. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

Keywords

CC5
Methicillin resistant Staphylococcus aureus
Panton-Valentine leucocidin
Sri Lanka
Whole genome sequencing

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.3389/fcimb.2019.00123

Fingerprint

Dive into the research topics of 'Genomic and Epidemiological Evidence of a Dominant Panton-Valentine Leucocidin-Positive Methicillin Resistant Staphylococcus aureus Lineage in Sri Lanka and Presence among Isolates from the United Kingdom and Australia'. Together they form a unique fingerprint.

Cite this

McTavish, S. M., Snow, S. J., Cook, E. C., Pichon, B., Coleman, S., Coombs, G. W., Pang, S., Arias, C. A., Díaz, L., Boldock, E., Davies, S., Udukala, M., Kearns, A., Siribaddana, S., & De Silva, T. I. (2019). Genomic and Epidemiological Evidence of a Dominant Panton-Valentine Leucocidin-Positive Methicillin Resistant Staphylococcus aureus Lineage in Sri Lanka and Presence among Isolates from the United Kingdom and Australia. Frontiers in Cellular and Infection Microbiology, 9(APR), Article 123. https://doi.org/10.3389/fcimb.2019.00123

@article{12a485d896de4201ae8b8efdc1438d99,

title = "Genomic and Epidemiological Evidence of a Dominant Panton-Valentine Leucocidin-Positive Methicillin Resistant Staphylococcus aureus Lineage in Sri Lanka and Presence among Isolates from the United Kingdom and Australia",

abstract = "Objective: To undertake the first detailed genomic analysis of methicillin-resistant Staphylococcus aureus (MRSA) isolated in Sri Lanka. Methods: A prospective observational study was performed on 94 MRSA isolates collected over a 4 months period from the Anuradhapura Teaching Hospital, Sri Lanka. Screening for mecA, mecC, and the Panton-Valentine leucocidin (PVL)-associated lukS-PV/lukF-PV genes and molecular characterization by spa typing was undertaken. Whole genome sequencing (WGS) and phylogenetic analysis was performed on selected multilocus sequence type (MLST) clonal complex 5 (CC5) isolates from Sri Lanka, England, Australia, and Argentina. Results: All 94 MRSA harbored the mecA gene. Nineteen spa types belonging to nine MLST clonal complexes were identified. Where origin of the sample was recorded, most isolates were from skin and soft tissue infections (70/91; 76.9\%), with fewer causing bacteremia (16/91; 17.6\%), empyema (3/91; 3.3\%) and osteomyelitis (2/91; 2.2\%). Sixty two (65.9\%) isolates were PVL positive with the majority (56 isolates; 90.3\%) belonging to a dominant CC5 lineage. This lineage, PVL-positive ST5-MRSA-IVc, was associated with both community and hospital-onset infections. Based on WGS, representative PVL-positive ST5-MRSA-IVc isolates from Sri Lanka, England and Australia formed a single phylogenetic clade, suggesting wide geographical circulation. Conclusions: We present the most detailed genomic analysis of MRSA isolated in Sri Lanka to date. The analysis identified a PVL-positive ST5-MRSA-IVc that is prevalent among MRSA causing clinical infections in Sri Lanka. Furthermore, this clone was also found among isolates from the United Kingdom and Australia.",

keywords = "CC5, Methicillin resistant Staphylococcus aureus, Panton-Valentine leucocidin, Sri Lanka, Whole genome sequencing",

author = "McTavish, \{Sharla M.\} and Snow, \{Sarah J.\} and Cook, \{Ellie C.\} and Bruno Pichon and Sarah Coleman and Coombs, \{Geoffrey W.\} and Stanley Pang and Arias, \{Cesar A.\} and Lorena D{\'i}az and Emma Boldock and Steve Davies and Mangala Udukala and Angela Kearns and Sisira Siribaddana and \{De Silva\}, \{Thushan I.\}",

note = "Funding Information: TdS is funded by a Wellcome Trust Intermediate Clinical Fellowship (110058/Z/15/Z). LD was supported by grants COL130871250417 and COL130874455850 from Colciencias. CA is funded through an NIH-NIAID grant K24 AI-AI121296, R01-AI134637, R01-AI093749 and R21 AI121519. During fieldwork, SJS and EC were supported by student grants from The Sheffield Medico-Chirurgical Society, The Sheffield Grammer School{\textquoteright} Trust and The 800th Lord Mayor{\textquoteright}s Anniversary Trust. Publisher Copyright: Copyright {\textcopyright} 2019 McTavish, Snow, Cook, Pichon, Coleman, Coombs, Pang, Arias, D{\'i}az, Boldock, Davies, Udukala, Kearns, Siribaddana and de Silva. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.",

year = "2019",

doi = "10.3389/fcimb.2019.00123",

language = "English",

volume = "9",

journal = "Frontiers in Cellular and Infection Microbiology",

issn = "2235-2988",

publisher = "Frontiers Media SA",

number = "APR",

}

McTavish, SM, Snow, SJ, Cook, EC, Pichon, B, Coleman, S, Coombs, GW, Pang, S, Arias, CA, Díaz, L, Boldock, E, Davies, S, Udukala, M, Kearns, A, Siribaddana, S & De Silva, TI 2019, 'Genomic and Epidemiological Evidence of a Dominant Panton-Valentine Leucocidin-Positive Methicillin Resistant Staphylococcus aureus Lineage in Sri Lanka and Presence among Isolates from the United Kingdom and Australia', Frontiers in Cellular and Infection Microbiology, vol. 9, no. APR, 123. https://doi.org/10.3389/fcimb.2019.00123

Genomic and Epidemiological Evidence of a Dominant Panton-Valentine Leucocidin-Positive Methicillin Resistant Staphylococcus aureus Lineage in Sri Lanka and Presence among Isolates from the United Kingdom and Australia. / McTavish, Sharla M.; Snow, Sarah J.; Cook, Ellie C. et al.
In: Frontiers in Cellular and Infection Microbiology, Vol. 9, No. APR, 123, 2019.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Genomic and Epidemiological Evidence of a Dominant Panton-Valentine Leucocidin-Positive Methicillin Resistant Staphylococcus aureus Lineage in Sri Lanka and Presence among Isolates from the United Kingdom and Australia

AU - McTavish, Sharla M.

AU - Snow, Sarah J.

AU - Cook, Ellie C.

AU - Pichon, Bruno

AU - Coleman, Sarah

AU - Coombs, Geoffrey W.

AU - Pang, Stanley

AU - Arias, Cesar A.

AU - Díaz, Lorena

AU - Boldock, Emma

AU - Davies, Steve

AU - Udukala, Mangala

AU - Kearns, Angela

AU - Siribaddana, Sisira

AU - De Silva, Thushan I.

N1 - Funding Information: TdS is funded by a Wellcome Trust Intermediate Clinical Fellowship (110058/Z/15/Z). LD was supported by grants COL130871250417 and COL130874455850 from Colciencias. CA is funded through an NIH-NIAID grant K24 AI-AI121296, R01-AI134637, R01-AI093749 and R21 AI121519. During fieldwork, SJS and EC were supported by student grants from The Sheffield Medico-Chirurgical Society, The Sheffield Grammer School’ Trust and The 800th Lord Mayor’s Anniversary Trust. Publisher Copyright: Copyright © 2019 McTavish, Snow, Cook, Pichon, Coleman, Coombs, Pang, Arias, Díaz, Boldock, Davies, Udukala, Kearns, Siribaddana and de Silva. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PY - 2019

Y1 - 2019

N2 - Objective: To undertake the first detailed genomic analysis of methicillin-resistant Staphylococcus aureus (MRSA) isolated in Sri Lanka. Methods: A prospective observational study was performed on 94 MRSA isolates collected over a 4 months period from the Anuradhapura Teaching Hospital, Sri Lanka. Screening for mecA, mecC, and the Panton-Valentine leucocidin (PVL)-associated lukS-PV/lukF-PV genes and molecular characterization by spa typing was undertaken. Whole genome sequencing (WGS) and phylogenetic analysis was performed on selected multilocus sequence type (MLST) clonal complex 5 (CC5) isolates from Sri Lanka, England, Australia, and Argentina. Results: All 94 MRSA harbored the mecA gene. Nineteen spa types belonging to nine MLST clonal complexes were identified. Where origin of the sample was recorded, most isolates were from skin and soft tissue infections (70/91; 76.9%), with fewer causing bacteremia (16/91; 17.6%), empyema (3/91; 3.3%) and osteomyelitis (2/91; 2.2%). Sixty two (65.9%) isolates were PVL positive with the majority (56 isolates; 90.3%) belonging to a dominant CC5 lineage. This lineage, PVL-positive ST5-MRSA-IVc, was associated with both community and hospital-onset infections. Based on WGS, representative PVL-positive ST5-MRSA-IVc isolates from Sri Lanka, England and Australia formed a single phylogenetic clade, suggesting wide geographical circulation. Conclusions: We present the most detailed genomic analysis of MRSA isolated in Sri Lanka to date. The analysis identified a PVL-positive ST5-MRSA-IVc that is prevalent among MRSA causing clinical infections in Sri Lanka. Furthermore, this clone was also found among isolates from the United Kingdom and Australia.

AB - Objective: To undertake the first detailed genomic analysis of methicillin-resistant Staphylococcus aureus (MRSA) isolated in Sri Lanka. Methods: A prospective observational study was performed on 94 MRSA isolates collected over a 4 months period from the Anuradhapura Teaching Hospital, Sri Lanka. Screening for mecA, mecC, and the Panton-Valentine leucocidin (PVL)-associated lukS-PV/lukF-PV genes and molecular characterization by spa typing was undertaken. Whole genome sequencing (WGS) and phylogenetic analysis was performed on selected multilocus sequence type (MLST) clonal complex 5 (CC5) isolates from Sri Lanka, England, Australia, and Argentina. Results: All 94 MRSA harbored the mecA gene. Nineteen spa types belonging to nine MLST clonal complexes were identified. Where origin of the sample was recorded, most isolates were from skin and soft tissue infections (70/91; 76.9%), with fewer causing bacteremia (16/91; 17.6%), empyema (3/91; 3.3%) and osteomyelitis (2/91; 2.2%). Sixty two (65.9%) isolates were PVL positive with the majority (56 isolates; 90.3%) belonging to a dominant CC5 lineage. This lineage, PVL-positive ST5-MRSA-IVc, was associated with both community and hospital-onset infections. Based on WGS, representative PVL-positive ST5-MRSA-IVc isolates from Sri Lanka, England and Australia formed a single phylogenetic clade, suggesting wide geographical circulation. Conclusions: We present the most detailed genomic analysis of MRSA isolated in Sri Lanka to date. The analysis identified a PVL-positive ST5-MRSA-IVc that is prevalent among MRSA causing clinical infections in Sri Lanka. Furthermore, this clone was also found among isolates from the United Kingdom and Australia.

KW - CC5

KW - Methicillin resistant Staphylococcus aureus

KW - Panton-Valentine leucocidin

KW - Sri Lanka

KW - Whole genome sequencing

UR - https://www.scopus.com/pages/publications/85066061643

U2 - 10.3389/fcimb.2019.00123

DO - 10.3389/fcimb.2019.00123

M3 - Article

C2 - 31080781

AN - SCOPUS:85066061643

SN - 2235-2988

VL - 9

JO - Frontiers in Cellular and Infection Microbiology

JF - Frontiers in Cellular and Infection Microbiology

IS - APR

M1 - 123

ER -

McTavish SM, Snow SJ, Cook EC, Pichon B, Coleman S, Coombs GW et al. Genomic and Epidemiological Evidence of a Dominant Panton-Valentine Leucocidin-Positive Methicillin Resistant Staphylococcus aureus Lineage in Sri Lanka and Presence among Isolates from the United Kingdom and Australia. Frontiers in Cellular and Infection Microbiology. 2019;9(APR):123. doi: 10.3389/fcimb.2019.00123

Genomic and Epidemiological Evidence of a Dominant Panton-Valentine Leucocidin-Positive Methicillin Resistant Staphylococcus aureus Lineage in Sri Lanka and Presence among Isolates from the United Kingdom and Australia

Abstract

Bibliographical note

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this