Genetic diversity of influenza A(H1N1)2009 virus circulating during the season 2010-2011 in Spain

Juan Ledesma; Francisco Pozo; Gabriel Reina; Miriam Blasco; Guadalupe Rodríguez; Milagrosa Montes; Isabel López-Miragaya; Carmen Salvador; Jordi Reina; Raúl Ortíz de Lejarazu; Pilar Egido; José López Barba; Concepción Delgado; María Teresa Cuevas; Inmaculada Casas

doi:10.1016/j.jcv.2011.09.032

Genetic diversity of influenza A(H1N1)2009 virus circulating during the season 2010-2011 in Spain

Juan Ledesma^*, Francisco Pozo, Gabriel Reina, Miriam Blasco, Guadalupe Rodríguez, Milagrosa Montes, Isabel López-Miragaya, Carmen Salvador, Jordi Reina, Raúl Ortíz de Lejarazu, Pilar Egido, José López Barba, Concepción Delgado, María Teresa Cuevas, Inmaculada Casas

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

Background: Genetic diversity of influenza A(H1N1)2009 viruses has been reported since the pandemic virus emerged in April 2009. Different genetic clades have been identified and defined based on amino acid substitutions found in the haemagglutinin (HA) protein sequences. In Spain, circulating influenza viruses are monitored each season by the regional laboratories enrolled in the Spanish Influenza Surveillance System (SISS). The analysis of the HA gene sequence helps to detect the genetic diversity and viral evolution. Objectives: To perform an analysis of the genetic diversity of influenza A(H1N1)2009 viruses circulating in Spain during the season 2010-2011 based on analysis of the HA sequence gene. Study design: Phylogenetic analysis based on the HA1 subunit of the haemagglutinin gene was carried out on 220 influenza A(H1N1)2009 viruses circulating during the season 2010-2011. Results: Six different genetic groups were identified among circulating A(H1N1)2009 viruses, five of them were previously reported during season 2010-2011. A new group, characterized by E172K and K308E changes and a proline at position 83, was observed in 12.27% of the Spanish viruses. Conclusion: Co-circulation of six different genetic groups of influenza A(H1N1)2009 viruses was identified in Spain during the season 2010-2011. Nevertheless, at this stage, none of the groups identified to date have resulted in significant antigenic changes according to data collected by World Health Organization Collaborating Centres for influenza surveillance.

Original language	English
Pages (from-to)	16-21
Number of pages	6
Journal	Journal of Clinical Virology
Volume	53
Issue number	1
DOIs	https://doi.org/10.1016/j.jcv.2011.09.032
Publication status	Published - Jan 2012
Externally published	Yes

Bibliographical note

Funding Information:
We would like to acknowledge all members of the Spanish Influenza Surveillance System for the contribution in this study. The authors acknowledge technical assistance from Ana Calderón, Nieves Cruz, Mónica Gónzalez-Esguevillas, Manuela Lopez-Valero, Mar Molinero, Silvia Moreno and Noelia Reyes in the National Influenza Centre-Madrid. This study was supported by grant MPY-1440/09 (Spanish Ministry of Science and Innovation), by grant between the Institute of Health Carlos III and the Spanish Ministry of Health for the surveillance of influenza viruses circulating in Spain, DGEG-1304/08-TS-13-B, and by grant PS09/00246 (Fondo de Investigaciones Sanitarias, ISCIII).

Funding Information:
This study was supported by grant PS09/00246 ( Fondo de Invesitgaciones Sanitarias, Spanish Ministry of Health and Social Policy ), by grant MPY-1440/09 ( Spanish Ministry of Science and Innovation ), an agreement signed between the Institute of Health Carlos III and the Spanish Ministry of Health and Social Policy for the surveillance of influenza viruses circulating in Spain, DGEG-1304/08-TS-13-B.

Keywords

Haemagglutinin
Influenza A(H1N1)2009
Phylogeny

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.jcv.2011.09.032

Cite this

Ledesma, J., Pozo, F., Reina, G., Blasco, M., Rodríguez, G., Montes, M., López-Miragaya, I., Salvador, C., Reina, J., Ortíz de Lejarazu, R., Egido, P., López Barba, J., Delgado, C., Cuevas, M. T., & Casas, I. (2012). Genetic diversity of influenza A(H1N1)2009 virus circulating during the season 2010-2011 in Spain. Journal of Clinical Virology, 53(1), 16-21. https://doi.org/10.1016/j.jcv.2011.09.032

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title = "Genetic diversity of influenza A(H1N1)2009 virus circulating during the season 2010-2011 in Spain",

abstract = "Background: Genetic diversity of influenza A(H1N1)2009 viruses has been reported since the pandemic virus emerged in April 2009. Different genetic clades have been identified and defined based on amino acid substitutions found in the haemagglutinin (HA) protein sequences. In Spain, circulating influenza viruses are monitored each season by the regional laboratories enrolled in the Spanish Influenza Surveillance System (SISS). The analysis of the HA gene sequence helps to detect the genetic diversity and viral evolution. Objectives: To perform an analysis of the genetic diversity of influenza A(H1N1)2009 viruses circulating in Spain during the season 2010-2011 based on analysis of the HA sequence gene. Study design: Phylogenetic analysis based on the HA1 subunit of the haemagglutinin gene was carried out on 220 influenza A(H1N1)2009 viruses circulating during the season 2010-2011. Results: Six different genetic groups were identified among circulating A(H1N1)2009 viruses, five of them were previously reported during season 2010-2011. A new group, characterized by E172K and K308E changes and a proline at position 83, was observed in 12.27% of the Spanish viruses. Conclusion: Co-circulation of six different genetic groups of influenza A(H1N1)2009 viruses was identified in Spain during the season 2010-2011. Nevertheless, at this stage, none of the groups identified to date have resulted in significant antigenic changes according to data collected by World Health Organization Collaborating Centres for influenza surveillance.",

keywords = "Haemagglutinin, Influenza A(H1N1)2009, Phylogeny",

author = "Juan Ledesma and Francisco Pozo and Gabriel Reina and Miriam Blasco and Guadalupe Rodr{\'i}guez and Milagrosa Montes and Isabel L{\'o}pez-Miragaya and Carmen Salvador and Jordi Reina and {Ort{\'i}z de Lejarazu}, Ra{\'u}l and Pilar Egido and {L{\'o}pez Barba}, Jos{\'e} and Concepci{\'o}n Delgado and Cuevas, {Mar{\'i}a Teresa} and Inmaculada Casas",

note = "Funding Information: We would like to acknowledge all members of the Spanish Influenza Surveillance System for the contribution in this study. The authors acknowledge technical assistance from Ana Calder{\'o}n, Nieves Cruz, M{\'o}nica G{\'o}nzalez-Esguevillas, Manuela Lopez-Valero, Mar Molinero, Silvia Moreno and Noelia Reyes in the National Influenza Centre-Madrid. This study was supported by grant MPY-1440/09 (Spanish Ministry of Science and Innovation), by grant between the Institute of Health Carlos III and the Spanish Ministry of Health for the surveillance of influenza viruses circulating in Spain, DGEG-1304/08-TS-13-B, and by grant PS09/00246 (Fondo de Investigaciones Sanitarias, ISCIII). Funding Information: This study was supported by grant PS09/00246 ( Fondo de Invesitgaciones Sanitarias, Spanish Ministry of Health and Social Policy ), by grant MPY-1440/09 ( Spanish Ministry of Science and Innovation ), an agreement signed between the Institute of Health Carlos III and the Spanish Ministry of Health and Social Policy for the surveillance of influenza viruses circulating in Spain, DGEG-1304/08-TS-13-B. ",

year = "2012",

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doi = "10.1016/j.jcv.2011.09.032",

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Ledesma, J, Pozo, F, Reina, G, Blasco, M, Rodríguez, G, Montes, M, López-Miragaya, I, Salvador, C, Reina, J, Ortíz de Lejarazu, R, Egido, P, López Barba, J, Delgado, C, Cuevas, MT & Casas, I 2012, 'Genetic diversity of influenza A(H1N1)2009 virus circulating during the season 2010-2011 in Spain', Journal of Clinical Virology, vol. 53, no. 1, pp. 16-21. https://doi.org/10.1016/j.jcv.2011.09.032

TY - JOUR

T1 - Genetic diversity of influenza A(H1N1)2009 virus circulating during the season 2010-2011 in Spain

AU - Ledesma, Juan

AU - Pozo, Francisco

AU - Reina, Gabriel

AU - Blasco, Miriam

AU - Rodríguez, Guadalupe

AU - Montes, Milagrosa

AU - López-Miragaya, Isabel

AU - Salvador, Carmen

AU - Reina, Jordi

AU - Ortíz de Lejarazu, Raúl

AU - Egido, Pilar

AU - López Barba, José

AU - Delgado, Concepción

AU - Cuevas, María Teresa

AU - Casas, Inmaculada

N1 - Funding Information: We would like to acknowledge all members of the Spanish Influenza Surveillance System for the contribution in this study. The authors acknowledge technical assistance from Ana Calderón, Nieves Cruz, Mónica Gónzalez-Esguevillas, Manuela Lopez-Valero, Mar Molinero, Silvia Moreno and Noelia Reyes in the National Influenza Centre-Madrid. This study was supported by grant MPY-1440/09 (Spanish Ministry of Science and Innovation), by grant between the Institute of Health Carlos III and the Spanish Ministry of Health for the surveillance of influenza viruses circulating in Spain, DGEG-1304/08-TS-13-B, and by grant PS09/00246 (Fondo de Investigaciones Sanitarias, ISCIII). Funding Information: This study was supported by grant PS09/00246 ( Fondo de Invesitgaciones Sanitarias, Spanish Ministry of Health and Social Policy ), by grant MPY-1440/09 ( Spanish Ministry of Science and Innovation ), an agreement signed between the Institute of Health Carlos III and the Spanish Ministry of Health and Social Policy for the surveillance of influenza viruses circulating in Spain, DGEG-1304/08-TS-13-B.

PY - 2012/1

Y1 - 2012/1

N2 - Background: Genetic diversity of influenza A(H1N1)2009 viruses has been reported since the pandemic virus emerged in April 2009. Different genetic clades have been identified and defined based on amino acid substitutions found in the haemagglutinin (HA) protein sequences. In Spain, circulating influenza viruses are monitored each season by the regional laboratories enrolled in the Spanish Influenza Surveillance System (SISS). The analysis of the HA gene sequence helps to detect the genetic diversity and viral evolution. Objectives: To perform an analysis of the genetic diversity of influenza A(H1N1)2009 viruses circulating in Spain during the season 2010-2011 based on analysis of the HA sequence gene. Study design: Phylogenetic analysis based on the HA1 subunit of the haemagglutinin gene was carried out on 220 influenza A(H1N1)2009 viruses circulating during the season 2010-2011. Results: Six different genetic groups were identified among circulating A(H1N1)2009 viruses, five of them were previously reported during season 2010-2011. A new group, characterized by E172K and K308E changes and a proline at position 83, was observed in 12.27% of the Spanish viruses. Conclusion: Co-circulation of six different genetic groups of influenza A(H1N1)2009 viruses was identified in Spain during the season 2010-2011. Nevertheless, at this stage, none of the groups identified to date have resulted in significant antigenic changes according to data collected by World Health Organization Collaborating Centres for influenza surveillance.

AB - Background: Genetic diversity of influenza A(H1N1)2009 viruses has been reported since the pandemic virus emerged in April 2009. Different genetic clades have been identified and defined based on amino acid substitutions found in the haemagglutinin (HA) protein sequences. In Spain, circulating influenza viruses are monitored each season by the regional laboratories enrolled in the Spanish Influenza Surveillance System (SISS). The analysis of the HA gene sequence helps to detect the genetic diversity and viral evolution. Objectives: To perform an analysis of the genetic diversity of influenza A(H1N1)2009 viruses circulating in Spain during the season 2010-2011 based on analysis of the HA sequence gene. Study design: Phylogenetic analysis based on the HA1 subunit of the haemagglutinin gene was carried out on 220 influenza A(H1N1)2009 viruses circulating during the season 2010-2011. Results: Six different genetic groups were identified among circulating A(H1N1)2009 viruses, five of them were previously reported during season 2010-2011. A new group, characterized by E172K and K308E changes and a proline at position 83, was observed in 12.27% of the Spanish viruses. Conclusion: Co-circulation of six different genetic groups of influenza A(H1N1)2009 viruses was identified in Spain during the season 2010-2011. Nevertheless, at this stage, none of the groups identified to date have resulted in significant antigenic changes according to data collected by World Health Organization Collaborating Centres for influenza surveillance.

KW - Haemagglutinin

KW - Influenza A(H1N1)2009

KW - Phylogeny

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JF - Journal of Clinical Virology

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ER -

Genetic diversity of influenza A(H1N1)2009 virus circulating during the season 2010-2011 in Spain

Abstract

Bibliographical note

Keywords

UN SDGs

Access to Document

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