Genetic analysis of acetan biosynthesis in Acetobacter xylinum: DNA sequence analysis of the aceM gene encoding an UDP-glucose dehydrogenase

Annette M. Griffin; Kirstin J. Edwards; Victor J. Morris; Michael J. Gasson

doi:10.1023/A:1018304412944

Genetic analysis of acetan biosynthesis in Acetobacter xylinum: DNA sequence analysis of the aceM gene encoding an UDP-glucose dehydrogenase

Annette M. Griffin^*, Kirstin J. Edwards, Victor J. Morris, Michael J. Gasson

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Analysis of the nucleotide sequence of a 1592 bp region. of Acetobacter xylinum genomic DNA involved in acetan biosynthesis revealed the presence of an open-reading frame (aceM) encoding a protein of 449 amino acids with a molecular weight of 48.5 kDa. The deduced amino acid sequence of aceM displayed high homology to the protein sequences of genes encoding UDP-glucose dehydrogenase (UGDH) activities from other organisms. AceM is likely to encode the UGDH involved in the biosynthesis of UDP-glucuronic acid required for acetan biosynthesis.

Original language	English
Pages (from-to)	469-474
Number of pages	6
Journal	Biotechnology Letters
Volume	19
Issue number	5
DOIs	https://doi.org/10.1023/A:1018304412944
Publication status	Published - 1997
Externally published	Yes

Bibliographical note

Funding Information:
This work was funded by the Biotechnology and Biological Sciences Research Council. The sequence has been deposited in the EMBL databank under the Accession Number Y11203.

Access to Document

10.1023/A:1018304412944

Cite this

@article{d92bbeb5370344dd9ab5c80671935e8d,

title = "Genetic analysis of acetan biosynthesis in Acetobacter xylinum: DNA sequence analysis of the aceM gene encoding an UDP-glucose dehydrogenase",

abstract = "Analysis of the nucleotide sequence of a 1592 bp region. of Acetobacter xylinum genomic DNA involved in acetan biosynthesis revealed the presence of an open-reading frame (aceM) encoding a protein of 449 amino acids with a molecular weight of 48.5 kDa. The deduced amino acid sequence of aceM displayed high homology to the protein sequences of genes encoding UDP-glucose dehydrogenase (UGDH) activities from other organisms. AceM is likely to encode the UGDH involved in the biosynthesis of UDP-glucuronic acid required for acetan biosynthesis.",

author = "Griffin, {Annette M.} and Edwards, {Kirstin J.} and Morris, {Victor J.} and Gasson, {Michael J.}",

note = "Funding Information: This work was funded by the Biotechnology and Biological Sciences Research Council. The sequence has been deposited in the EMBL databank under the Accession Number Y11203.",

year = "1997",

doi = "10.1023/A:1018304412944",

language = "English",

volume = "19",

pages = "469--474",

journal = "Biotechnology Letters",

issn = "0141-5492",

publisher = "Springer",

number = "5",

}

TY - JOUR

T1 - Genetic analysis of acetan biosynthesis in Acetobacter xylinum

T2 - DNA sequence analysis of the aceM gene encoding an UDP-glucose dehydrogenase

AU - Griffin, Annette M.

AU - Edwards, Kirstin J.

AU - Morris, Victor J.

AU - Gasson, Michael J.

N1 - Funding Information: This work was funded by the Biotechnology and Biological Sciences Research Council. The sequence has been deposited in the EMBL databank under the Accession Number Y11203.

PY - 1997

Y1 - 1997

N2 - Analysis of the nucleotide sequence of a 1592 bp region. of Acetobacter xylinum genomic DNA involved in acetan biosynthesis revealed the presence of an open-reading frame (aceM) encoding a protein of 449 amino acids with a molecular weight of 48.5 kDa. The deduced amino acid sequence of aceM displayed high homology to the protein sequences of genes encoding UDP-glucose dehydrogenase (UGDH) activities from other organisms. AceM is likely to encode the UGDH involved in the biosynthesis of UDP-glucuronic acid required for acetan biosynthesis.

AB - Analysis of the nucleotide sequence of a 1592 bp region. of Acetobacter xylinum genomic DNA involved in acetan biosynthesis revealed the presence of an open-reading frame (aceM) encoding a protein of 449 amino acids with a molecular weight of 48.5 kDa. The deduced amino acid sequence of aceM displayed high homology to the protein sequences of genes encoding UDP-glucose dehydrogenase (UGDH) activities from other organisms. AceM is likely to encode the UGDH involved in the biosynthesis of UDP-glucuronic acid required for acetan biosynthesis.

UR - http://www.scopus.com/inward/record.url?scp=0030943565&partnerID=8YFLogxK

U2 - 10.1023/A:1018304412944

DO - 10.1023/A:1018304412944

M3 - Article

AN - SCOPUS:0030943565

SN - 0141-5492

VL - 19

SP - 469

EP - 474

JO - Biotechnology Letters

JF - Biotechnology Letters

IS - 5

ER -

Genetic analysis of acetan biosynthesis in Acetobacter xylinum: DNA sequence analysis of the aceM gene encoding an UDP-glucose dehydrogenase

Abstract

Bibliographical note

Access to Document

Other files and links

Fingerprint

Cite this