Generation of a novel polysaccharide by inactivation of the aceP gene from the acetan biosynthetic pathway in Acetobacter xylinum

K. J. Edwards, A. J. Jay, I. J. Colquhoun, V. J. Morris, M. J. Gasson, A. M. Griffin*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)

Abstract

The acetan biosynthetic pathway in Acetobacter xylinum is an ideal model system for engineering novel bacterial polysaccharides. To genetically manipulate this pathway, an Acetobacter strain (CKE5), more susceptible to gene-transfer methodologies, was developed. A new gene, aceP, involved in acetan biosynthesis was identified, sequenced and shown to have homology at the amino acid level with β-D-glucosyl transferases from a number of different organisms. Disruption of aceP in strain CKE confirmed the function assigned above and was used to engineer a novel polysaccharide with a pentasaccharide repeat unit.

Original languageEnglish
Pages (from-to)1499-1506
Number of pages8
JournalMicrobiology
Volume145
Issue number6
DOIs
Publication statusPublished - Jun 1999
Externally publishedYes

Keywords

  • aceP gene
  • Acetan
  • Acetobacter xylinum
  • EPS biosynthesis
  • Novel EPS

Fingerprint

Dive into the research topics of 'Generation of a novel polysaccharide by inactivation of the aceP gene from the acetan biosynthetic pathway in Acetobacter xylinum'. Together they form a unique fingerprint.

Cite this