Abstract
Fluorescent amplified fragment length polymorphism (fAFLP) is based on the selective PCR amplification of restriction fragments from a digest of total genomic DNA. Genomic DNA extracted from a purified bacterial isolate is completely digested with two endonucleases generating fragments which are ligated to specific double-stranded adaptors. The ligated fragments are then amplified by PCR using fluorescently labelled primers. Fluorescent amplified fragments are separated by size on an automated sequencer with a size standard. fAFLP is a rapid, highly reproducible technique which can be used to discriminate and subtype Listeria monocytogenes strains.
Original language | English |
---|---|
Pages (from-to) | 95-101 |
Number of pages | 7 |
Journal | Methods in molecular biology (Clifton, N.J.) |
Volume | 1157 |
DOIs | |
Publication status | Published - 2014 |
Bibliographical note
Publisher Copyright:© Springer Science+Business Media New York 2014.
Keywords
- AFLP
- Fragment analysis
- Listeria monocytogenes
- Restriction