TY - JOUR
T1 - Fluorescent amplified fragment length polymorphism (fAFLP) analysis of listeria monocytogenes
AU - Amar, Corinne
N1 - Publisher Copyright:
© Springer Science+Business Media New York 2014.
Copyright:
Copyright 2015 Elsevier B.V., All rights reserved.
PY - 2014
Y1 - 2014
N2 - Fluorescent amplified fragment length polymorphism (fAFLP) is based on the selective PCR amplification of restriction fragments from a digest of total genomic DNA. Genomic DNA extracted from a purified bacterial isolate is completely digested with two endonucleases generating fragments which are ligated to specific double-stranded adaptors. The ligated fragments are then amplified by PCR using fluorescently labelled primers. Fluorescent amplified fragments are separated by size on an automated sequencer with a size standard. fAFLP is a rapid, highly reproducible technique which can be used to discriminate and subtype Listeria monocytogenes strains.
AB - Fluorescent amplified fragment length polymorphism (fAFLP) is based on the selective PCR amplification of restriction fragments from a digest of total genomic DNA. Genomic DNA extracted from a purified bacterial isolate is completely digested with two endonucleases generating fragments which are ligated to specific double-stranded adaptors. The ligated fragments are then amplified by PCR using fluorescently labelled primers. Fluorescent amplified fragments are separated by size on an automated sequencer with a size standard. fAFLP is a rapid, highly reproducible technique which can be used to discriminate and subtype Listeria monocytogenes strains.
KW - AFLP
KW - Fragment analysis
KW - Listeria monocytogenes
KW - Restriction
UR - http://www.scopus.com/inward/record.url?scp=84922015519&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-0703-8_8
DO - 10.1007/978-1-4939-0703-8_8
M3 - Article
C2 - 24792551
AN - SCOPUS:84922015519
VL - 1157
SP - 95
EP - 101
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
SN - 1064-3745
ER -