TY - JOUR
T1 - First worldwide proficiency study on variable-number tandem-repeat typing of Mycobacterium tuberculosis complex strains
AU - De Beer, Jessica L.
AU - Kremer, Kristin
AU - Ködmön, Csaba
AU - Supply, Philip
AU - Van Soolingen, Dick
AU - Alexander, David
AU - Augustynowicz-Kopeć, Ewa
AU - Bidovec-Stojkovic, Urska
AU - Boeree, Martin J.
AU - Brown, Timothy
AU - Cirillo, Daniela M.
AU - Cruz, Laura
AU - David, Susana
AU - Diaz, Raul
AU - Dou, Horng Yunn
AU - Evans, Jason T.
AU - Fauville-Dufaux, Maryse
AU - Fitzgibbon, Margaret M.
AU - García De Viedma, Darío
AU - Globan, Maria
AU - Groenheit, Ramona
AU - Haanperä-Heikkinen, Marjo
AU - Indra, Alexander
AU - Kam, Kai Man
AU - Kramer, Rebecca
AU - Mei, Shang
AU - Niemann, Stefan
AU - Obrovac, Mihaela
AU - Rasmussen, Erik M.
AU - Refrégier, Guislaine
AU - Robledo, Jaime A.
AU - Samper, Sofia
AU - Sharma, Meenu K.
AU - Sougakoff, Wladimir
AU - Stakenas, Petras
AU - Stavrum, Ruth
AU - Suffys, Philip N.
AU - Trenkler, Juraj
AU - Wada, Takayuki
AU - Warren, Robin M.
PY - 2012/3
Y1 - 2012/3
N2 - Although variable-number tandem-repeat (VNTR) typing has gained recognition as the new standard for the DNA fingerprinting of Mycobacterium tuberculosis complex (MTBC) isolates, external quality control programs have not yet been developed. Therefore, we organized the first multicenter proficiency study on 24-locus VNTR typing. Sets of 30 DNAs of MTBC strains, including 10 duplicate DNA samples, were distributed among 37 participating laboratories in 30 different countries worldwide. Twenty-four laboratories used an in-house-adapted method with fragment sizing by gel electrophoresis or an automated DNA analyzer, nine laboratories used a commercially available kit, and four laboratories used other methods. The intra- and interlaboratory reproducibilities of VNTR typing varied from 0% to 100%, with averages of 72% and 60%, respectively. Twenty of the 37 laboratories failed to amplify particular VNTR loci; if these missing results were ignored, the number of laboratories with 100% interlaboratory reproducibility increased from 1 to 5. The average interlaboratory reproducibility of VNTR typing using a commercial kit was better (88%) than that of in-house-adapted methods using a DNA analyzer (70%) or gel electrophoresis (50%). Eleven laboratories using in-house-adapted manual typing or automated typing scored inter- and intralaboratory reproducibilities of 80% or higher, which suggests that these approaches can be used in a reliable way. In conclusion, this first multicenter study has documented the worldwide quality of VNTR typing of MTBC strains and highlights the importance of international quality control to improve genotyping in the future.
AB - Although variable-number tandem-repeat (VNTR) typing has gained recognition as the new standard for the DNA fingerprinting of Mycobacterium tuberculosis complex (MTBC) isolates, external quality control programs have not yet been developed. Therefore, we organized the first multicenter proficiency study on 24-locus VNTR typing. Sets of 30 DNAs of MTBC strains, including 10 duplicate DNA samples, were distributed among 37 participating laboratories in 30 different countries worldwide. Twenty-four laboratories used an in-house-adapted method with fragment sizing by gel electrophoresis or an automated DNA analyzer, nine laboratories used a commercially available kit, and four laboratories used other methods. The intra- and interlaboratory reproducibilities of VNTR typing varied from 0% to 100%, with averages of 72% and 60%, respectively. Twenty of the 37 laboratories failed to amplify particular VNTR loci; if these missing results were ignored, the number of laboratories with 100% interlaboratory reproducibility increased from 1 to 5. The average interlaboratory reproducibility of VNTR typing using a commercial kit was better (88%) than that of in-house-adapted methods using a DNA analyzer (70%) or gel electrophoresis (50%). Eleven laboratories using in-house-adapted manual typing or automated typing scored inter- and intralaboratory reproducibilities of 80% or higher, which suggests that these approaches can be used in a reliable way. In conclusion, this first multicenter study has documented the worldwide quality of VNTR typing of MTBC strains and highlights the importance of international quality control to improve genotyping in the future.
UR - http://www.scopus.com/inward/record.url?scp=84857386498&partnerID=8YFLogxK
U2 - 10.1128/JCM.00607-11
DO - 10.1128/JCM.00607-11
M3 - Article
C2 - 22170917
AN - SCOPUS:84857386498
SN - 0095-1137
VL - 50
SP - 662
EP - 669
JO - Journal of Clinical Microbiology
JF - Journal of Clinical Microbiology
IS - 3
ER -
