First external quality assurance of antibody diagnostic for SARS-new coronavirus

Matthias Niedrig; Katrin Leitmeyer; Wilina Lim; Malik Peiris; John S. Mackenzie; Maria Zambon

doi:10.1016/j.jcv.2005.01.004

First external quality assurance of antibody diagnostic for SARS-new coronavirus

Matthias Niedrig^*
, Katrin Leitmeyer
, Wilina Lim
, Malik Peiris
, John S. Mackenzie
, Maria Zambon

^*Corresponding author for this work

Respiratory Virus Unit

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

To confirm an infection with the new coronavirus (SARS-CoV) causing the severe acute respiratory syndrome (SARS) diagnostic assays for detection of SARS-CoV specific antibody are necessary. To evaluate the diagnostic performance of laboratories an external quality assurance (EQA) study was performed in 2004. Participating laboratories (9/20) correctly detected anti-SARS antibodies in serum samples without false positive results in an immunofluorescence assay. In contrast, only 4/13 laboratories detected most of the anti-SARS antibody positive samples without false positive results using enzyme immunoassays (EIA) and/or immunoblot. The overall results clearly demonstrate that serological diagnosis of SARS-CoV remains at an early stage of development, with further technical improvements required, particularly with respect to the use of SARS specific EIAs.

Original language	English
Pages (from-to)	22-25
Number of pages	4
Journal	Journal of Clinical Virology
Volume	34
Issue number	1
DOIs	https://doi.org/10.1016/j.jcv.2005.01.004
Publication status	Published - Sept 2005

Bibliographical note

Funding Information:
The work was partially supported by the EC grant SPC 20002396. The author especially thanks Dr. Angela Merianos, the SARS focal point at WHO, Geneva for providing information and support.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Antibody diagnostic
EQA
External quality assurance
SARS

Access to Document

10.1016/j.jcv.2005.01.004

Cite this

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title = "First external quality assurance of antibody diagnostic for SARS-new coronavirus",

abstract = "To confirm an infection with the new coronavirus (SARS-CoV) causing the severe acute respiratory syndrome (SARS) diagnostic assays for detection of SARS-CoV specific antibody are necessary. To evaluate the diagnostic performance of laboratories an external quality assurance (EQA) study was performed in 2004. Participating laboratories (9/20) correctly detected anti-SARS antibodies in serum samples without false positive results in an immunofluorescence assay. In contrast, only 4/13 laboratories detected most of the anti-SARS antibody positive samples without false positive results using enzyme immunoassays (EIA) and/or immunoblot. The overall results clearly demonstrate that serological diagnosis of SARS-CoV remains at an early stage of development, with further technical improvements required, particularly with respect to the use of SARS specific EIAs.",

keywords = "Antibody diagnostic, EQA, External quality assurance, SARS",

author = "Matthias Niedrig and Katrin Leitmeyer and Wilina Lim and Malik Peiris and Mackenzie, \{John S.\} and Maria Zambon",

note = "Funding Information: The work was partially supported by the EC grant SPC 20002396. The author especially thanks Dr. Angela Merianos, the SARS focal point at WHO, Geneva for providing information and support. ",

year = "2005",

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doi = "10.1016/j.jcv.2005.01.004",

language = "English",

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T1 - First external quality assurance of antibody diagnostic for SARS-new coronavirus

AU - Niedrig, Matthias

AU - Leitmeyer, Katrin

AU - Lim, Wilina

AU - Peiris, Malik

AU - Mackenzie, John S.

AU - Zambon, Maria

N1 - Funding Information: The work was partially supported by the EC grant SPC 20002396. The author especially thanks Dr. Angela Merianos, the SARS focal point at WHO, Geneva for providing information and support.

PY - 2005/9

Y1 - 2005/9

N2 - To confirm an infection with the new coronavirus (SARS-CoV) causing the severe acute respiratory syndrome (SARS) diagnostic assays for detection of SARS-CoV specific antibody are necessary. To evaluate the diagnostic performance of laboratories an external quality assurance (EQA) study was performed in 2004. Participating laboratories (9/20) correctly detected anti-SARS antibodies in serum samples without false positive results in an immunofluorescence assay. In contrast, only 4/13 laboratories detected most of the anti-SARS antibody positive samples without false positive results using enzyme immunoassays (EIA) and/or immunoblot. The overall results clearly demonstrate that serological diagnosis of SARS-CoV remains at an early stage of development, with further technical improvements required, particularly with respect to the use of SARS specific EIAs.

AB - To confirm an infection with the new coronavirus (SARS-CoV) causing the severe acute respiratory syndrome (SARS) diagnostic assays for detection of SARS-CoV specific antibody are necessary. To evaluate the diagnostic performance of laboratories an external quality assurance (EQA) study was performed in 2004. Participating laboratories (9/20) correctly detected anti-SARS antibodies in serum samples without false positive results in an immunofluorescence assay. In contrast, only 4/13 laboratories detected most of the anti-SARS antibody positive samples without false positive results using enzyme immunoassays (EIA) and/or immunoblot. The overall results clearly demonstrate that serological diagnosis of SARS-CoV remains at an early stage of development, with further technical improvements required, particularly with respect to the use of SARS specific EIAs.

KW - Antibody diagnostic

KW - EQA

KW - External quality assurance

KW - SARS

UR - https://www.scopus.com/pages/publications/23744507266

U2 - 10.1016/j.jcv.2005.01.004

DO - 10.1016/j.jcv.2005.01.004

M3 - Article

C2 - 16087120

AN - SCOPUS:23744507266

SN - 1386-6532

VL - 34

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JO - Journal of Clinical Virology

JF - Journal of Clinical Virology

IS - 1

ER -

First external quality assurance of antibody diagnostic for SARS-new coronavirus

Abstract

Bibliographical note

UN SDGs

Keywords

Access to Document

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