Farnesol increases the activity of echinocandins against Candida auris biofilms

Fruzsina Nagy; Zoltán Tóth; Lajos Daróczi; Adrien Székely; Andrew Borman; László Majoros; Renátó Kovács

doi:10.1093/mmy/myz057

Farnesol increases the activity of echinocandins against Candida auris biofilms

Fruzsina Nagy, Zoltán Tóth, Lajos Daróczi, Adrien Székely, Andrew Borman, László Majoros, Renátó Kovács^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

25 Citations (Scopus)

Abstract

Candida auris biofilms exhibit decreased susceptibility to echinocandins, which is associated with poorer clinical outcomes. Farnesol is a quorum-sensing molecule enhancing the activity of antifungals; therefore, we evaluated the in vitro effect of farnesol with anidulafungin, caspofungin, or micafungin against biofilms using fractional inhibitory concentration indexes (FICI), Bliss independence model, LIVE/DEAD-assay and scanning electron microscopy. Based on mathematical models, farnesol caused synergism in eleven out of twelve cases (FICIs range 0.133-0.507; Bliss synergy volume range 70.39-204.6 μM2%). This was confirmed by microscope images of combination-exposed biofilms. Our study showed the prominent effect of farnesol with echinocandins against C. auris biofilms.

Original language	English
Pages (from-to)	404-407
Number of pages	4
Journal	Medical Mycology
Volume	58
Issue number	3
DOIs	https://doi.org/10.1093/mmy/myz057
Publication status	Published - 1 Apr 2020

Bibliographical note

Funding Information:
The study was supported by the EFOP-3.6.3-VEKOP-16-2017-00009 program. Zoltán Tóth and Fruzsina Nagy were supported by the ÚNKP-18-3 New National Excellence Program of the Ministry of Human Capacities.

Funding Information:
L. Majoros received conference travel grants from MSD, Astellas, and Pfizer. All other authors report no conflicts of interest.

Publisher Copyright:
© 2019 The Author(s) 2019. Published by Oxford University Press on behalf of The International Society for Human and Animal Mycology.

Keywords

Candida auris
biofilm
farnesol
lock therapy
synergy

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1093/mmy/myz057

Cite this

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title = "Farnesol increases the activity of echinocandins against Candida auris biofilms",

abstract = "Candida auris biofilms exhibit decreased susceptibility to echinocandins, which is associated with poorer clinical outcomes. Farnesol is a quorum-sensing molecule enhancing the activity of antifungals; therefore, we evaluated the in vitro effect of farnesol with anidulafungin, caspofungin, or micafungin against biofilms using fractional inhibitory concentration indexes (FICI), Bliss independence model, LIVE/DEAD-assay and scanning electron microscopy. Based on mathematical models, farnesol caused synergism in eleven out of twelve cases (FICIs range 0.133-0.507; Bliss synergy volume range 70.39-204.6 μM2%). This was confirmed by microscope images of combination-exposed biofilms. Our study showed the prominent effect of farnesol with echinocandins against C. auris biofilms.",

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AU - Borman, Andrew

AU - Majoros, László

AU - Kovács, Renátó

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N2 - Candida auris biofilms exhibit decreased susceptibility to echinocandins, which is associated with poorer clinical outcomes. Farnesol is a quorum-sensing molecule enhancing the activity of antifungals; therefore, we evaluated the in vitro effect of farnesol with anidulafungin, caspofungin, or micafungin against biofilms using fractional inhibitory concentration indexes (FICI), Bliss independence model, LIVE/DEAD-assay and scanning electron microscopy. Based on mathematical models, farnesol caused synergism in eleven out of twelve cases (FICIs range 0.133-0.507; Bliss synergy volume range 70.39-204.6 μM2%). This was confirmed by microscope images of combination-exposed biofilms. Our study showed the prominent effect of farnesol with echinocandins against C. auris biofilms.

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Farnesol increases the activity of echinocandins against Candida auris biofilms

Abstract

Bibliographical note

Keywords

UN SDGs

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