Candida auris biofilms exhibit decreased susceptibility to echinocandins, which is associated with poorer clinical outcomes. Farnesol is a quorum-sensing molecule enhancing the activity of antifungals; therefore, we evaluated the in vitro effect of farnesol with anidulafungin, caspofungin, or micafungin against biofilms using fractional inhibitory concentration indexes (FICI), Bliss independence model, LIVE/DEAD-assay and scanning electron microscopy. Based on mathematical models, farnesol caused synergism in eleven out of twelve cases (FICIs range 0.133-0.507; Bliss synergy volume range 70.39-204.6 μM2%). This was confirmed by microscope images of combination-exposed biofilms. Our study showed the prominent effect of farnesol with echinocandins against C. auris biofilms.
Bibliographical noteFunding Information:
The study was supported by the EFOP-3.6.3-VEKOP-16-2017-00009 program. Zoltán Tóth and Fruzsina Nagy were supported by the ÚNKP-18-3 New National Excellence Program of the Ministry of Human Capacities.
L. Majoros received conference travel grants from MSD, Astellas, and Pfizer. All other authors report no conflicts of interest.
© 2019 The Author(s) 2019. Published by Oxford University Press on behalf of The International Society for Human and Animal Mycology.
- Candida auris
- lock therapy