Expression, purification, crystallization and preliminary crystallographic analysis of a putative Clostridium difficile surface protein Cwp19

Jonathan M. Kirby; Nethaji Thiyagarajan; April K. Roberts; Clifford C. Shone; K. Ravi Acharya

doi:10.1107/S1744309111016770

Expression, purification, crystallization and preliminary crystallographic analysis of a putative Clostridium difficile surface protein Cwp19

Jonathan M. Kirby, Nethaji Thiyagarajan, April K. Roberts, Clifford C. Shone, K. Ravi Acharya^*

^*Corresponding author for this work

Research & Technical Project Managers

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Cwp19 is a putatively surface-located protein from Clostridium difficile. A recombinant N-terminal protein (residues 27-401) lacking the signal peptide and the C-terminal cell-wall-binding repeats (PFam04122) was crystallized using the sitting-drop vapour-diffusion method and diffracted to 2 Å resolution. The crystal appeared to belong to the primitive monoclinic space group P2 ₁, with unit-cell parameters a = 109.1, b = 61.2, c = 109.2 Å, β = 111.85°, and is estimated to contain two molecules of Cwp19 per asymmetric unit.

Original language	English
Pages (from-to)	762-767
Number of pages	6
Journal	Acta Crystallographica Section F: Structural Biology and Crystallization Communications
Volume	67
Issue number	7
DOIs	https://doi.org/10.1107/S1744309111016770
Publication status	Published - Jul 2011

Keywords

Clostridium difficile
Cwp19
surface proteins

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10.1107/S1744309111016770

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Kirby, J. M., Thiyagarajan, N., Roberts, A. K., Shone, C. C., & Acharya, K. R. (2011). Expression, purification, crystallization and preliminary crystallographic analysis of a putative Clostridium difficile surface protein Cwp19. Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 67(7), 762-767. https://doi.org/10.1107/S1744309111016770

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title = "Expression, purification, crystallization and preliminary crystallographic analysis of a putative Clostridium difficile surface protein Cwp19",

abstract = "Cwp19 is a putatively surface-located protein from Clostridium difficile. A recombinant N-terminal protein (residues 27-401) lacking the signal peptide and the C-terminal cell-wall-binding repeats (PFam04122) was crystallized using the sitting-drop vapour-diffusion method and diffracted to 2 {\AA} resolution. The crystal appeared to belong to the primitive monoclinic space group P2 1, with unit-cell parameters a = 109.1, b = 61.2, c = 109.2 {\AA}, β = 111.85°, and is estimated to contain two molecules of Cwp19 per asymmetric unit.",

keywords = "Clostridium difficile, Cwp19, surface proteins",

author = "Kirby, {Jonathan M.} and Nethaji Thiyagarajan and Roberts, {April K.} and Shone, {Clifford C.} and Acharya, {K. Ravi}",

year = "2011",

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doi = "10.1107/S1744309111016770",

language = "English",

volume = "67",

pages = "762--767",

journal = "Acta Crystallographica Section F: Structural Biology and Crystallization Communications",

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Kirby, JM, Thiyagarajan, N, Roberts, AK, Shone, CC & Acharya, KR 2011, 'Expression, purification, crystallization and preliminary crystallographic analysis of a putative Clostridium difficile surface protein Cwp19', Acta Crystallographica Section F: Structural Biology and Crystallization Communications, vol. 67, no. 7, pp. 762-767. https://doi.org/10.1107/S1744309111016770

Expression, purification, crystallization and preliminary crystallographic analysis of a putative Clostridium difficile surface protein Cwp19. / Kirby, Jonathan M.; Thiyagarajan, Nethaji; Roberts, April K. et al.
In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Vol. 67, No. 7, 07.2011, p. 762-767.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Expression, purification, crystallization and preliminary crystallographic analysis of a putative Clostridium difficile surface protein Cwp19

AU - Kirby, Jonathan M.

AU - Thiyagarajan, Nethaji

AU - Roberts, April K.

AU - Shone, Clifford C.

AU - Acharya, K. Ravi

PY - 2011/7

Y1 - 2011/7

N2 - Cwp19 is a putatively surface-located protein from Clostridium difficile. A recombinant N-terminal protein (residues 27-401) lacking the signal peptide and the C-terminal cell-wall-binding repeats (PFam04122) was crystallized using the sitting-drop vapour-diffusion method and diffracted to 2 Å resolution. The crystal appeared to belong to the primitive monoclinic space group P2 1, with unit-cell parameters a = 109.1, b = 61.2, c = 109.2 Å, β = 111.85°, and is estimated to contain two molecules of Cwp19 per asymmetric unit.

AB - Cwp19 is a putatively surface-located protein from Clostridium difficile. A recombinant N-terminal protein (residues 27-401) lacking the signal peptide and the C-terminal cell-wall-binding repeats (PFam04122) was crystallized using the sitting-drop vapour-diffusion method and diffracted to 2 Å resolution. The crystal appeared to belong to the primitive monoclinic space group P2 1, with unit-cell parameters a = 109.1, b = 61.2, c = 109.2 Å, β = 111.85°, and is estimated to contain two molecules of Cwp19 per asymmetric unit.

KW - Clostridium difficile

KW - Cwp19

KW - surface proteins

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DO - 10.1107/S1744309111016770

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AN - SCOPUS:79960939327

SN - 1744-3091

VL - 67

SP - 762

EP - 767

JO - Acta Crystallographica Section F: Structural Biology and Crystallization Communications

JF - Acta Crystallographica Section F: Structural Biology and Crystallization Communications

IS - 7

ER -

Expression, purification, crystallization and preliminary crystallographic analysis of a putative Clostridium difficile surface protein Cwp19

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