TY - JOUR
T1 - Expression, purification, crystallization and preliminary crystallographic analysis of a putative Clostridium difficile surface protein Cwp19
AU - Kirby, Jonathan M.
AU - Thiyagarajan, Nethaji
AU - Roberts, April K.
AU - Shone, Clifford C.
AU - Acharya, K. Ravi
PY - 2011/7
Y1 - 2011/7
N2 - Cwp19 is a putatively surface-located protein from Clostridium difficile. A recombinant N-terminal protein (residues 27-401) lacking the signal peptide and the C-terminal cell-wall-binding repeats (PFam04122) was crystallized using the sitting-drop vapour-diffusion method and diffracted to 2 Å resolution. The crystal appeared to belong to the primitive monoclinic space group P2 1, with unit-cell parameters a = 109.1, b = 61.2, c = 109.2 Å, β = 111.85°, and is estimated to contain two molecules of Cwp19 per asymmetric unit.
AB - Cwp19 is a putatively surface-located protein from Clostridium difficile. A recombinant N-terminal protein (residues 27-401) lacking the signal peptide and the C-terminal cell-wall-binding repeats (PFam04122) was crystallized using the sitting-drop vapour-diffusion method and diffracted to 2 Å resolution. The crystal appeared to belong to the primitive monoclinic space group P2 1, with unit-cell parameters a = 109.1, b = 61.2, c = 109.2 Å, β = 111.85°, and is estimated to contain two molecules of Cwp19 per asymmetric unit.
KW - Clostridium difficile
KW - Cwp19
KW - surface proteins
UR - http://www.scopus.com/inward/record.url?scp=79960939327&partnerID=8YFLogxK
U2 - 10.1107/S1744309111016770
DO - 10.1107/S1744309111016770
M3 - Article
C2 - 21795789
AN - SCOPUS:79960939327
VL - 67
SP - 762
EP - 767
JO - Acta Crystallographica Section F: Structural Biology and Crystallization Communications
JF - Acta Crystallographica Section F: Structural Biology and Crystallization Communications
SN - 1744-3091
IS - 7
ER -