Expression of the nucleocapsid protein of Dugbe virus and antigenic cross-reactions with other nairoviruses

Vernon K. Ward; Anthony C. Marriott; Thalia Polyzoni; Ali A. El-Ghorr; Anthony Antoniadis; Patricia A. Nuttall

doi:10.1016/0168-1702(92)90009-X

Expression of the nucleocapsid protein of Dugbe virus and antigenic cross-reactions with other nairoviruses

Vernon K. Ward, Anthony C. Marriott, Thalia Polyzoni, Ali A. El-Ghorr, Anthony Antoniadis, Patricia A. Nuttall^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

The small (S) RNA segment of Dugbe (DUG) virus (Nairovirus, Bunyaviridae) encodes a single protein, the nucleocapsid (N) protein, of M_r 49.4 kDa. cDNA derived from the complete coding region for the N protein was cloned into Autographa californica nuclear polyhedrosis virus (AcNPV) under control of the polyhedrin promoter and used to infect Spodoptera frugiperda insect cells. Western blotting analysis using monoclonal antibodies demonstrated the production of DUG N protein in the infected cells. Monoclonal and polyclonal antibodies to the N protein of Crimean-Congo haemorrhagic fever (CCHF) virus were found to cross-react weakly with the baculovirus expressed DUG N protein by Western blotting. When used in an enzyme linked immunoassay (ELISA), the DUG N protein reacted with polyclonal mouse immune ascitic fluids raised against either CCHF or Hazara viruses (both members of the CCHF serogroup of nairoviruses). Cross-reactions between DUG virus (Nairobi sheep disease serogroup) and members of other nairovirus serogroups were not detected.

Original language	English
Pages (from-to)	223-229
Number of pages	7
Journal	Virus Research
Volume	24
Issue number	2
DOIs	https://doi.org/10.1016/0168-1702(92)90009-X
Publication status	Published - Jul 1992
Externally published	Yes

Keywords

Baculovirus expression
Dugbe virus
Nairovirus cross-reactivity

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/0168-1702(92)90009-X

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title = "Expression of the nucleocapsid protein of Dugbe virus and antigenic cross-reactions with other nairoviruses",

abstract = "The small (S) RNA segment of Dugbe (DUG) virus (Nairovirus, Bunyaviridae) encodes a single protein, the nucleocapsid (N) protein, of Mr 49.4 kDa. cDNA derived from the complete coding region for the N protein was cloned into Autographa californica nuclear polyhedrosis virus (AcNPV) under control of the polyhedrin promoter and used to infect Spodoptera frugiperda insect cells. Western blotting analysis using monoclonal antibodies demonstrated the production of DUG N protein in the infected cells. Monoclonal and polyclonal antibodies to the N protein of Crimean-Congo haemorrhagic fever (CCHF) virus were found to cross-react weakly with the baculovirus expressed DUG N protein by Western blotting. When used in an enzyme linked immunoassay (ELISA), the DUG N protein reacted with polyclonal mouse immune ascitic fluids raised against either CCHF or Hazara viruses (both members of the CCHF serogroup of nairoviruses). Cross-reactions between DUG virus (Nairobi sheep disease serogroup) and members of other nairovirus serogroups were not detected.",

keywords = "Baculovirus expression, Dugbe virus, Nairovirus cross-reactivity",

author = "Ward, {Vernon K.} and Marriott, {Anthony C.} and Thalia Polyzoni and El-Ghorr, {Ali A.} and Anthony Antoniadis and Nuttall, {Patricia A.}",

year = "1992",

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doi = "10.1016/0168-1702(92)90009-X",

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T1 - Expression of the nucleocapsid protein of Dugbe virus and antigenic cross-reactions with other nairoviruses

AU - Ward, Vernon K.

AU - Marriott, Anthony C.

AU - Polyzoni, Thalia

AU - El-Ghorr, Ali A.

AU - Antoniadis, Anthony

AU - Nuttall, Patricia A.

PY - 1992/7

Y1 - 1992/7

N2 - The small (S) RNA segment of Dugbe (DUG) virus (Nairovirus, Bunyaviridae) encodes a single protein, the nucleocapsid (N) protein, of Mr 49.4 kDa. cDNA derived from the complete coding region for the N protein was cloned into Autographa californica nuclear polyhedrosis virus (AcNPV) under control of the polyhedrin promoter and used to infect Spodoptera frugiperda insect cells. Western blotting analysis using monoclonal antibodies demonstrated the production of DUG N protein in the infected cells. Monoclonal and polyclonal antibodies to the N protein of Crimean-Congo haemorrhagic fever (CCHF) virus were found to cross-react weakly with the baculovirus expressed DUG N protein by Western blotting. When used in an enzyme linked immunoassay (ELISA), the DUG N protein reacted with polyclonal mouse immune ascitic fluids raised against either CCHF or Hazara viruses (both members of the CCHF serogroup of nairoviruses). Cross-reactions between DUG virus (Nairobi sheep disease serogroup) and members of other nairovirus serogroups were not detected.

AB - The small (S) RNA segment of Dugbe (DUG) virus (Nairovirus, Bunyaviridae) encodes a single protein, the nucleocapsid (N) protein, of Mr 49.4 kDa. cDNA derived from the complete coding region for the N protein was cloned into Autographa californica nuclear polyhedrosis virus (AcNPV) under control of the polyhedrin promoter and used to infect Spodoptera frugiperda insect cells. Western blotting analysis using monoclonal antibodies demonstrated the production of DUG N protein in the infected cells. Monoclonal and polyclonal antibodies to the N protein of Crimean-Congo haemorrhagic fever (CCHF) virus were found to cross-react weakly with the baculovirus expressed DUG N protein by Western blotting. When used in an enzyme linked immunoassay (ELISA), the DUG N protein reacted with polyclonal mouse immune ascitic fluids raised against either CCHF or Hazara viruses (both members of the CCHF serogroup of nairoviruses). Cross-reactions between DUG virus (Nairobi sheep disease serogroup) and members of other nairovirus serogroups were not detected.

KW - Baculovirus expression

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KW - Nairovirus cross-reactivity

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ER -

Expression of the nucleocapsid protein of Dugbe virus and antigenic cross-reactions with other nairoviruses

Abstract

Keywords

UN SDGs

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