Evolutionary clues from comparative analysis of Mycobacterium tuberculosis variable-number tandem repeat sequences within genetic families

Nicola Thorne; Anthony Underwood; Saheer Gharbia; Cath Arnold

doi:10.1016/j.meegid.2006.09.006

Evolutionary clues from comparative analysis of Mycobacterium tuberculosis variable-number tandem repeat sequences within genetic families

Nicola Thorne, Anthony Underwood, Saheer Gharbia, Cath Arnold^*

^*Corresponding author for this work

Gastrointestinal Infections

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Mycobacterium tuberculosis is one of the most successful bacterial pathogens in the history of mankind, and the study of the evolutionary and genetic behaviour of this organism is ongoing. The potential of variable-number tandem repeat (VNTR) regions to provide insight into the evolutionary past of this organism has yet to be evaluated. The aim of this study was to investigate diversity occurring within VNTR loci within established evolutionary lineages. Mycobacterial interspersed repetitive unit (MIRU) 4 and 26 nucleotide sequencing was undertaken revealing significant differences in VNTR discriminatory power and distribution of allelic types within defined sub-lineages, within a moderately discriminant and a highly discriminant locus, respectively. These findings indicate potential allele bias at these loci within the defined groups dependent on the genotype of their progenitor strain, and the patterns of distribution suggest that a step-wise contraction/expansion process is responsible for VNTR repeat evolution. The results also identified a possible incidence of genetic drift within major genetic group 2 (MGG2), which has a point mutation at locus MIRU26 in a clonal subgroup.

Original language	English
Pages (from-to)	239-246
Number of pages	8
Journal	Infection, Genetics and Evolution
Volume	7
Issue number	2
DOIs	https://doi.org/10.1016/j.meegid.2006.09.006
Publication status	Published - Mar 2007

Bibliographical note

Funding Information:
This publication made use of the supplemental data from Arnold et al. (2006) which included data from eight recent studies ( Sreevatsan et al., 1997; Brosch et al., 2002; Cowan et al., 2002; Filliol et al., 2002; Sola et al., 2003a,b; Mokrousov et al., 2004; Sun et al., 2004a,b ) as well as the MIRU-VNTR Typing website ( http://www.ibl.fr/mirus.mirus.html ). This database was developed by Dr P. Supply and E. Savine and sited at the INSERM U447, at the Institut de Biologie de Lille/Institut Pasteur de Lille ( Supply et al., 2001 ). The development of this database was funded by the French Ministère de la Recherche.

Keywords

Clonal
Evolution
Lineage
Mycobacterium tuberculosis
Nucleotide polymorphism
Phylogeny
Repeats
VNTR

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.meegid.2006.09.006

Cite this

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title = "Evolutionary clues from comparative analysis of Mycobacterium tuberculosis variable-number tandem repeat sequences within genetic families",

abstract = "Mycobacterium tuberculosis is one of the most successful bacterial pathogens in the history of mankind, and the study of the evolutionary and genetic behaviour of this organism is ongoing. The potential of variable-number tandem repeat (VNTR) regions to provide insight into the evolutionary past of this organism has yet to be evaluated. The aim of this study was to investigate diversity occurring within VNTR loci within established evolutionary lineages. Mycobacterial interspersed repetitive unit (MIRU) 4 and 26 nucleotide sequencing was undertaken revealing significant differences in VNTR discriminatory power and distribution of allelic types within defined sub-lineages, within a moderately discriminant and a highly discriminant locus, respectively. These findings indicate potential allele bias at these loci within the defined groups dependent on the genotype of their progenitor strain, and the patterns of distribution suggest that a step-wise contraction/expansion process is responsible for VNTR repeat evolution. The results also identified a possible incidence of genetic drift within major genetic group 2 (MGG2), which has a point mutation at locus MIRU26 in a clonal subgroup.",

keywords = "Clonal, Evolution, Lineage, Mycobacterium tuberculosis, Nucleotide polymorphism, Phylogeny, Repeats, VNTR",

author = "Nicola Thorne and Anthony Underwood and Saheer Gharbia and Cath Arnold",

note = "Funding Information: This publication made use of the supplemental data from Arnold et al. (2006) which included data from eight recent studies ( Sreevatsan et al., 1997; Brosch et al., 2002; Cowan et al., 2002; Filliol et al., 2002; Sola et al., 2003a,b; Mokrousov et al., 2004; Sun et al., 2004a,b ) as well as the MIRU-VNTR Typing website ( http://www.ibl.fr/mirus.mirus.html ). This database was developed by Dr P. Supply and E. Savine and sited at the INSERM U447, at the Institut de Biologie de Lille/Institut Pasteur de Lille ( Supply et al., 2001 ). The development of this database was funded by the French Minist{\`e}re de la Recherche. ",

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AU - Thorne, Nicola

AU - Underwood, Anthony

AU - Gharbia, Saheer

AU - Arnold, Cath

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KW - Repeats

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Evolutionary clues from comparative analysis of Mycobacterium tuberculosis variable-number tandem repeat sequences within genetic families

Abstract

Bibliographical note

Keywords

UN SDGs

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