Evidence for Msh2 haploinsufficiency in mice revealed by MNU-induced sister-chromatid exchange analysis

S. D. Bouffler; N. Hofland; R. Cox; R. Fodde

doi:10.1054/bjoc.2000.1422

Evidence for Msh2 haploinsufficiency in mice revealed by MNU-induced sister-chromatid exchange analysis

S. D. Bouffler^*, N. Hofland, R. Cox, R. Fodde

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

The role of Msh2 in chromosome stability has been investigated in a targeted mouse model for HNPCC, Msh2Δ7N. Chromosome aberration frequencies were similar in bone marrow of Msh2(+/+), Msh2(+/-) and Msh2(-/-) mice and no differential effects of in vivo X-irradiation were noted. By contrast, the induction of sister-chromatid exchanges (SCEs) by methyl nitrosourea (MNU) was reduced in Msh2(-/-) and Msh2(+/-) cells to ~20% and ~45% wild-type levels respectively indicating a phenotypic effect of haploinsufficiency of the mouse Msh2 gene. (C) 2000 Cancer Research Campaign.

Original language	English
Pages (from-to)	1291-1294
Number of pages	4
Journal	British Journal of Cancer
Volume	83
Issue number	10
DOIs	https://doi.org/10.1054/bjoc.2000.1422
Publication status	Published - 2000

Keywords

Alkylating agent
Ionizing radiation
Mismatch repair
Msh2
Sister-chromatid exchange

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1054/bjoc.2000.1422

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title = "Evidence for Msh2 haploinsufficiency in mice revealed by MNU-induced sister-chromatid exchange analysis",

abstract = "The role of Msh2 in chromosome stability has been investigated in a targeted mouse model for HNPCC, Msh2Δ7N. Chromosome aberration frequencies were similar in bone marrow of Msh2(+/+), Msh2(+/-) and Msh2(-/-) mice and no differential effects of in vivo X-irradiation were noted. By contrast, the induction of sister-chromatid exchanges (SCEs) by methyl nitrosourea (MNU) was reduced in Msh2(-/-) and Msh2(+/-) cells to ~20% and ~45% wild-type levels respectively indicating a phenotypic effect of haploinsufficiency of the mouse Msh2 gene. (C) 2000 Cancer Research Campaign.",

keywords = "Alkylating agent, Ionizing radiation, Mismatch repair, Msh2, Sister-chromatid exchange",

author = "Bouffler, {S. D.} and N. Hofland and R. Cox and R. Fodde",

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doi = "10.1054/bjoc.2000.1422",

language = "English",

volume = "83",

pages = "1291--1294",

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}

TY - JOUR

T1 - Evidence for Msh2 haploinsufficiency in mice revealed by MNU-induced sister-chromatid exchange analysis

AU - Bouffler, S. D.

AU - Hofland, N.

AU - Cox, R.

AU - Fodde, R.

PY - 2000

Y1 - 2000

N2 - The role of Msh2 in chromosome stability has been investigated in a targeted mouse model for HNPCC, Msh2Δ7N. Chromosome aberration frequencies were similar in bone marrow of Msh2(+/+), Msh2(+/-) and Msh2(-/-) mice and no differential effects of in vivo X-irradiation were noted. By contrast, the induction of sister-chromatid exchanges (SCEs) by methyl nitrosourea (MNU) was reduced in Msh2(-/-) and Msh2(+/-) cells to ~20% and ~45% wild-type levels respectively indicating a phenotypic effect of haploinsufficiency of the mouse Msh2 gene. (C) 2000 Cancer Research Campaign.

AB - The role of Msh2 in chromosome stability has been investigated in a targeted mouse model for HNPCC, Msh2Δ7N. Chromosome aberration frequencies were similar in bone marrow of Msh2(+/+), Msh2(+/-) and Msh2(-/-) mice and no differential effects of in vivo X-irradiation were noted. By contrast, the induction of sister-chromatid exchanges (SCEs) by methyl nitrosourea (MNU) was reduced in Msh2(-/-) and Msh2(+/-) cells to ~20% and ~45% wild-type levels respectively indicating a phenotypic effect of haploinsufficiency of the mouse Msh2 gene. (C) 2000 Cancer Research Campaign.

KW - Alkylating agent

KW - Ionizing radiation

KW - Mismatch repair

KW - Msh2

KW - Sister-chromatid exchange

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U2 - 10.1054/bjoc.2000.1422

DO - 10.1054/bjoc.2000.1422

M3 - Article

C2 - 11044352

AN - SCOPUS:0033741837

VL - 83

SP - 1291

EP - 1294

JO - British Journal of Cancer

JF - British Journal of Cancer

SN - 0007-0920

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ER -

Evidence for Msh2 haploinsufficiency in mice revealed by MNU-induced sister-chromatid exchange analysis

Abstract

Keywords

UN SDGs

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