Evaluation of the biofire filmarray biothreat-E test (v2.5) for rapid identification of ebola virus disease in heat-treated blood samples obtained in Sierra Leone and the United Kingdom

Simon A. Weller*, Daniel Bailey, Steven Matthews, Sarah Lumley, Angela Sweed, Derren Ready, Gary Eltringham, Jade Richards, Richard Vipond, Roman Lukaszewski, Phillippa M. Payne, Emma Aarons, Andrew Simpson, Emma J. Hutley, Timothy Brooks

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

29 Citations (Scopus)

Abstract

Rapid Ebola virus (EBOV) detection is crucial for appropriate patient management and care. The performance of the FilmArray BioThreat-E test (v2.5) using whole-blood samples was evaluated in Sierra Leone and the United Kingdom and was compared with results generated by a real-time Ebola Zaire PCR reference method. Samples were tested in diagnostic laboratories upon availability, included successive samples from individual patients, and were heat treated to facilitate EBOV inactivation prior to PCR. The BioThreat-E test had a sensitivity of 84% (confidence interval [CI], 64% to 95%) and a specificity of 89% (CI, 73% to 97%) in Sierra Leone (n=60; 44 patients) and a sensitivity of 75% (CI, 19% to 99%) and a specificity of 100% (CI, 97% to 100%) in the United Kingdom (n=108; 70 patients) compared to the reference real-time PCR. Statistical analysis (Fisher's exact test) indicated there was no significant difference between the methods at the 99% confidence level in either country. In 9 discrepant results (5 real-time PCR positives and BioThreat-E test negatives and 4 real-time PCR negatives and BioThreat-E test positives), the majority (n=8) were obtained from samples with an observed or probable low viral load. The FilmArray BioThreat-E test (v2.5) therefore provides an attractive option for laboratories (either in austere field settings or in countries with an advanced technological infrastructure) which do not routinely offer an EBOV diagnostic capability.

Original languageEnglish
Pages (from-to)114-119
Number of pages6
JournalJournal of Clinical Microbiology
Volume54
Issue number1
DOIs
Publication statusPublished - Jan 2016

Bibliographical note

Publisher Copyright:
© 2015, American Society for Microbiology. All Rights Reserved.

Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.

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