Evaluation of a nine-locus variable-number tandem-repeat scheme for typing of Pseudomonas aeruginosa

J. F. Turton*, S. E. Turton, L. Yearwood, S. Yarde, M. E. Kaufmann, T. L. Pitt

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

49 Citations (Scopus)

Abstract

Repeat numbers at nine variable-number tandem-repeat (VNTR) loci were determined for 177 isolates of Pseudomonas aeruginosa representing 77 strains distinguished by pulsed-field gel electrophoresis (PFGE). Eight loci provided for discrimination similar to that provided by PFGE, with variation at the ninth locus (ms61) sometimes allowing discrimination within a PFGE-defined type. The Liverpool and Midlands 1 strains, which are common among patients with cystic fibrosis in the UK, could be unambiguously identified by their characteristic VNTR profiles. In rare cases, the repeat number at the ninth locus alone provided discrimination among isolates that were distinct according to PFGE. In each case, the two isolates shared the same blaOXA-50-like allele and belonged to the same oprD sequence type group, supporting the VNTR results in suggesting that they are similar.

Original languageEnglish
Pages (from-to)1111-1116
Number of pages6
JournalClinical Microbiology and Infection
Volume16
Issue number8
DOIs
Publication statusPublished - Aug 2010

Bibliographical note

Funding Information:
We are grateful to the Society for General Microbiology, UK, for funding a vacation studentship to S. Turton to work on this project. None of the authors have any conflicting or dual interests.

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