TY - JOUR
T1 - Evaluation of a gold-silver staining method for detection and identification of Candida species by light microscopy
AU - Cailliez, J. C.
AU - Boudrissa, A.
AU - MacKenzie, Donald
AU - Poulain, D.
N1 - Copyright:
Copyright 2007 Elsevier B.V., All rights reserved.
PY - 1990/12
Y1 - 1990/12
N2 - A gold-silver staining procedure was evaluated for detection of Candida species of medical importance. Probes were prepared by coupling lectins or antibodies (polyclonal and monoclonal) directly or indirectly to colloidal gold particles. Structures reacting to these probes were specifically revealed by light microscopy in cells present in infected kidney tissue sections or in isolated yeast cells on glass slides. Definition, contrast and sensitivity were of a high order. Preliminary data showed that it was possible, using discriminating dilutions, to identify cells from different species of the genus Candida, grown in vitro, according to their ability to stain with polyclonal monospecific antisera. The advantages of gold-silver staining compared with other staining procedures currently used in routine mycological laboratories are its sensitivity, good definition, ease and rapidity, and long conservation of reaction. It is suggested that the procedure has applications for research and identification of yeasts in clinical samples.
AB - A gold-silver staining procedure was evaluated for detection of Candida species of medical importance. Probes were prepared by coupling lectins or antibodies (polyclonal and monoclonal) directly or indirectly to colloidal gold particles. Structures reacting to these probes were specifically revealed by light microscopy in cells present in infected kidney tissue sections or in isolated yeast cells on glass slides. Definition, contrast and sensitivity were of a high order. Preliminary data showed that it was possible, using discriminating dilutions, to identify cells from different species of the genus Candida, grown in vitro, according to their ability to stain with polyclonal monospecific antisera. The advantages of gold-silver staining compared with other staining procedures currently used in routine mycological laboratories are its sensitivity, good definition, ease and rapidity, and long conservation of reaction. It is suggested that the procedure has applications for research and identification of yeasts in clinical samples.
UR - http://www.scopus.com/inward/record.url?scp=0025607826&partnerID=8YFLogxK
U2 - 10.1007/BF01967504
DO - 10.1007/BF01967504
M3 - Article
C2 - 2073899
AN - SCOPUS:0025607826
SN - 0934-9723
VL - 9
SP - 886
EP - 891
JO - European Journal of Clinical Microbiology and Infectious Diseases
JF - European Journal of Clinical Microbiology and Infectious Diseases
IS - 12
ER -
