European multicenter evaluation of commercial enzyme immunoassays for detecting norovirus antigen in fecal samples

Jim J. Gray*, Evelyne Kohli, Franco M. Ruggeri, Harry Vennema, Alicia Sánchez-Fauquier, Eckart Schreier, Chris I. Gallimore, Miren Iturriza-Gomara, Helene Giraudon, Pierre Pothier, Ilaria Di Bartolo, Nadia Inglese, Erwin De Bruin, Bas Van Der Veer, Silvia Moreno, Vanessa Montero, Marí C. De Llano, Marina Höhne, Sabine M. Diedrich

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

84 Citations (Scopus)

Abstract

A total of 2,254 fecal samples were tested in a European multicenter evaluation of commercially available norovirus antigen detection assays. Two commercial enzyme immunoassays, IDEIA Norovirus (Oxoid; Thermo Fisher Scientific, Ely, United Kingdom) and RIDASCREEN Norovirus (R-Biopharm, Darmstadt, Germany), were included in the evaluation, and their performance was compared with the results of reverse transcription-PCR (RT-PCR). Included in the evaluation were samples collected in sporadic cases of gastroenteritis, samples from outbreaks in which two or more samples were collected, well-characterized samples representing genotypes currently cocirculating within Europe, and samples collected from patients with gastroenteritis caused by a pathogen other than norovirus. The sensitivities and specificities of the IDEIA Norovirus and RIDA-SCREEN Norovirus assays were 58.93 and 43.81% and 93.91 and 9637%, respectively, compared with RT-PCR. The sensitivities of both assays for outbreak investigations improved when six or more samples from an outbreak were examined. The IDEIA Norovirus assay exhibited reactivity to a broader range of norovirus genotypes than the RIDASCREEN Norovirus assay, which showed genotype-dependent sensitivities. The results indicate that, if used, these assays should serve as screening assays and the results should be confirmed by RT-PCR.

Original languageEnglish
Pages (from-to)1349-1355
Number of pages7
JournalClinical and Vaccine Immunology
Volume14
Issue number10
DOIs
Publication statusPublished - Oct 2007

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