Abstract
We introduce a new method for the analysis of enzyme-linked immunosorbent assay (ELISA) data. The new method can use data near the asymptotes and does not give undue weight to responses on the flatter parts of the dose-response curve. We apply it to simulated data and to two real-world assays and show it is more accurate and more precise than the traditional interpolation method. In particular, the new method works much better for very low-concentration samples for which the traditional method is often unable to give a result.
Original language | English |
---|---|
Article number | 112836 |
Journal | Journal of Immunological Methods |
Volume | 486 |
DOIs | |
Publication status | Published - Nov 2020 |
Bibliographical note
Publisher Copyright:© 2020 Elsevier B.V.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
Keywords
- Concentration estimation
- ELISA
- ELISA statistics
- Immunoassay