Escherichia coli bacteraemia: 2 years of prospective regional surveillance (2010-12)

Carolyne Horner*, W. Fawley, K. Morris, P. Parnell, M. Denton, M. Wilcox

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

50 Citations (Scopus)

Abstract

Objectives: To determine the prevalence of antibiotic resistance and the epidemiology of Escherichia coli bacteraemia isolates across the Yorkshire and Humber National Health Service region over 2 years. Methods: Ten percent of all E. coli blood culture isolateswere collected per month from 14 laboratories across the Yorkshire and Humber region. Individual laboratories submitted antibiotic susceptibility data and isolateswere retested centrally using the VITEK2® system (bioMérieux, France). Isolates were also characterized using PCR to test for the presence of sequences encoding extended-spectrumβ-lactamases (ESBLs) and genotyped using amplified fragment length polymorphism (AFLP). Selected isolates were further characterized using multilocus sequence typing. Results: Between July 2010 and June 2012, 770 isolateswere examined: 63%, 40%, 14% and 7%of isolates were non-susceptible to ampicillin, trimethoprim, ciprofloxacin and gentamicin, respectively. Eight percent of isolates (n=63) were ESBL positive; CTX-M group 1 enzymes were the most common (68%). There was a fluctuating trend in the prevalence of resistance to amoxicillin/clavulanic acid (MIC>8 mg/L): July-September 2010, 16%; July-September 2011, 38%; and April-June 2012, 22%. AFLP identified 106 types. The majority of isolates belonged to one of two AFLP types: AFLP 1 [sequence type (ST) 131; 17%] and AFLP 2 (ST73; 18%). ST131 and ST73 were both associated with hospital- and community-onset bacteraemia, and with urinary, hepatobiliary and gastrointestinal sources of infection. ESBL-positive isolates were predominantly ST131 (60%). Conclusions: Continued surveillance of antibiotic resistance among E. coli bacteraemia isolates is necessary to enhance these early baseline data. The variable prevalence of resistance to amoxicillin/clavulanic acid raises concerns, as both E. coli bacteraemia and empirical use of this antibiotic are common.

Original languageEnglish
Article numberdkt333
Pages (from-to)59-66
Number of pages8
JournalJournal of Antimicrobial Chemotherapy
Volume69
Issue number1
DOIs
Publication statusPublished - Jan 2014

Keywords

  • Antibiotic susceptibility
  • Epidemiology
  • Genetic background

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