TY - JOUR
T1 - Epidemic of carbapenem-resistant Klebsiella pneumoniae in Europe is driven by nosocomial spread
AU - the EuSCAPE Working Group
AU - the ESGEM Study Group
AU - David, Sophia
AU - Reuter, Sandra
AU - Harris, Simon R.
AU - Glasner, Corinna
AU - Feltwell, Theresa
AU - Argimon, Silvia
AU - Abudahab, Khalil
AU - Goater, Richard
AU - Giani, Tommaso
AU - Errico, Giulia
AU - Aspbury, Marianne
AU - Sjunnebo, Sara
AU - Koraqi, Andi
AU - Lacej, Denada
AU - Apfalter, Petra
AU - Hartl, Rainer
AU - Glupczynski, Youri
AU - Huang, Te Din
AU - Strateva, Tanya
AU - Marteva-Proevska, Yuliya
AU - Andrasevic, Arjana Tambic
AU - Butic, Iva
AU - Pieridou-Bagatzouni, Despo
AU - Maikanti-Charalampous, Panagiota
AU - Hrabak, Jaroslav
AU - Zemlickova, Helena
AU - Hammerum, Anette
AU - Jakobsen, Lotte
AU - Ivanova, Marina
AU - Pavelkovich, Anastasia
AU - Jalava, Jari
AU - Österblad, Monica
AU - Dortet, Laurent
AU - Vaux, Sophie
AU - Kaase, Martin
AU - Gatermann, Sören G.
AU - Vatopoulos, Alkiviadis
AU - Tryfinopoulou, Kyriaki
AU - Tóth, Ákos
AU - Jánvári, Laura
AU - Boo, Teck Wee
AU - McGrath, Elaine
AU - Carmeli, Yehuda
AU - Adler, Amos
AU - Pantosti, Annalisa
AU - Monaco, Monica
AU - Raka, Lul
AU - Kurti, Arsim
AU - Balode, Arta
AU - Woodford, Neil
N1 - Funding Information:
We thank the Pathogen Informatics Group and Core Sequencing Facility at the Wellcome Sanger Institute for their contributions to the study. We also thank F. Bosma and M. Zigterman from the Medical Microbiology and Infection Prevention Department of the University Medical Center Groningen for their support in assembling the isolate collection. This work was funded by The Centre for Genomic Pathogen Surveillance, Wellcome Genome Campus, Wellcome (grant nos. 098051 and 099202) and the NIHR Global Health Research Unit on Genomic Surveillance of Antimicrobial Resistance (NIHR 16/136/111). The EuSCAPE project was funded by ECDC through a specific framework contract (ECDC/2012/055) following an open call for tender (OJ/25/04/2012-PROC/2012/036). This work was carried out on behalf of the ESCMID Study Group for Epidemiological Markers (ESGEM); the full list of members is provided in the Supplementary Information.
Publisher Copyright:
© 2019, The Author(s), under exclusive licence to Springer Nature Limited.
PY - 2019/11/1
Y1 - 2019/11/1
N2 - Public health interventions to control the current epidemic of carbapenem-resistant Klebsiella pneumoniae rely on a comprehensive understanding of its emergence and spread over a wide range of geographical scales. We analysed the genome sequences and epidemiological data of >1,700 K. pneumoniae samples isolated from patients in 244 hospitals in 32 countries during the European Survey of Carbapenemase-Producing Enterobacteriaceae. We demonstrate that carbapenemase acquisition is the main cause of carbapenem resistance and that it occurred across diverse phylogenetic backgrounds. However, 477 of 682 (69.9%) carbapenemase-positive isolates are concentrated in four clonal lineages, sequence types 11, 15, 101, 258/512 and their derivatives. Combined analysis of the genetic and geographic distances between isolates with different β-lactam resistance determinants suggests that the propensity of K. pneumoniae to spread in hospital environments correlates with the degree of resistance and that carbapenemase-positive isolates have the highest transmissibility. Indeed, we found that over half of the hospitals that contributed carbapenemase-positive isolates probably experienced within-hospital transmission, and interhospital spread is far more frequent within, rather than between, countries. Finally, we propose a value of 21 for the number of single nucleotide polymorphisms that optimizes the discrimination of hospital clusters and detail the international spread of the successful epidemic lineage, ST258/512.
AB - Public health interventions to control the current epidemic of carbapenem-resistant Klebsiella pneumoniae rely on a comprehensive understanding of its emergence and spread over a wide range of geographical scales. We analysed the genome sequences and epidemiological data of >1,700 K. pneumoniae samples isolated from patients in 244 hospitals in 32 countries during the European Survey of Carbapenemase-Producing Enterobacteriaceae. We demonstrate that carbapenemase acquisition is the main cause of carbapenem resistance and that it occurred across diverse phylogenetic backgrounds. However, 477 of 682 (69.9%) carbapenemase-positive isolates are concentrated in four clonal lineages, sequence types 11, 15, 101, 258/512 and their derivatives. Combined analysis of the genetic and geographic distances between isolates with different β-lactam resistance determinants suggests that the propensity of K. pneumoniae to spread in hospital environments correlates with the degree of resistance and that carbapenemase-positive isolates have the highest transmissibility. Indeed, we found that over half of the hospitals that contributed carbapenemase-positive isolates probably experienced within-hospital transmission, and interhospital spread is far more frequent within, rather than between, countries. Finally, we propose a value of 21 for the number of single nucleotide polymorphisms that optimizes the discrimination of hospital clusters and detail the international spread of the successful epidemic lineage, ST258/512.
UR - http://www.scopus.com/inward/record.url?scp=85069915359&partnerID=8YFLogxK
U2 - 10.1038/s41564-019-0492-8
DO - 10.1038/s41564-019-0492-8
M3 - Article
C2 - 31358985
AN - SCOPUS:85069915359
SN - 2058-5276
VL - 4
SP - 1919
EP - 1929
JO - Nature Microbiology
JF - Nature Microbiology
IS - 11
ER -
