Emergence of Multidrug-Resistant Escherichia coli Producing CTX-M, MCR-1, and FosA in Retail Food From Egypt

Hazem Ramadan; Ahmed M. Soliman; Lari M. Hiott; Mohammed Elbediwi; Tiffanie A. Woodley; Marie A. Chattaway; Claire Jenkins; Jonathan G. Frye; Charlene R. Jackson

doi:10.3389/fcimb.2021.681588

Emergence of Multidrug-Resistant Escherichia coli Producing CTX-M, MCR-1, and FosA in Retail Food From Egypt

Hazem Ramadan^*, Ahmed M. Soliman, Lari M. Hiott, Mohammed Elbediwi, Tiffanie A. Woodley, Marie A. Chattaway, Claire Jenkins, Jonathan G. Frye, Charlene R. Jackson^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

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Abstract

In this study, multidrug-resistant (MDR) Escherichia coli isolates from retail food and humans assigned into similar Multilocus Sequence Types (MLST) were analyzed using whole genome sequencing (WGS). In silico analysis of assembled sequences revealed the existence of multiple resistance genes among the examined E. coli isolates. Of the six CTX-M-producing isolates from retail food, bla (CTX-M-14) was the prevalent variant identified (83.3%, 5/6). Two plasmid-mediated fosfomycin resistance genes, fosA3, and fosA4, were detected from retail food isolates (one each from chicken and beef), where fosA4 was identified in the chicken isolate 82CH that also carried the colistin resistance gene mcr-1. The bla (CTX-M-14) and fosA genes in retail food isolates were located adjacent to insertion sequences ISEcp1 and IS26, respectively. Sequence analysis of the reconstructed mcr-1 plasmid (p82CH) showed 96-97% identity to mcr-1-carrying IncI2 plasmids previously identified in human and food E. coli isolates from Egypt. Hierarchical clustering of core genome MLST (HierCC) revealed clustering of chicken isolate 82CH, co-harboring mcr-1 and fosA4 genes, with a chicken E. coli isolate from China at the HC200 level (

Original language	English
Article number	681588
Number of pages	9
Journal	Frontiers in Cellular and Infection Microbiology
Volume	11
DOIs	https://doi.org/10.3389/fcimb.2021.681588
Publication status	Published - 13 Jul 2021

Bibliographical note

Funding Information: This work has been funded by the U.S. Department of Agriculture (USDA) project 6040-32000-079-00-D. The Science, Technology and Innovation Fund Authority (STIFA) of Egypt (Short term fellowship, ID 25449) partially supported this work.

The authors acknowledge the Daniel Turnberg UK/Middle East
Travel Fellowship for funding the visit of Dr. Hazem Ramadan to
Public Health England, UK.

Open Access: Copyright © 2021 Ramadan, Soliman, Hiott, Elbediwi, Woodley, Chattaway, Jenkins, Frye and Jackson. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the
copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

Publisher Copyright: © Copyright © 2021 Ramadan, Soliman, Hiott, Elbediwi, Woodley, Chattaway, Jenkins, Frye and Jackson.

Citation: Ramadan, Hazem, et al. "Emergence of Multidrug-Resistant Escherichia coli Producing CTX-M, MCR-1, and FosA in Retail Food From Egypt." Frontiers in cellular and infection microbiology 11 (2021).

DOI: https://doi.org/10.3389/fcimb.2021.681588

Keywords

Escherichia coli
cgMLST clustering
fosA
genetic context
mcr-1
retail food
whole genome sequencing

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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Cite this

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title = "Emergence of Multidrug-Resistant Escherichia coli Producing CTX-M, MCR-1, and FosA in Retail Food From Egypt",

abstract = "In this study, multidrug-resistant (MDR) Escherichia coli isolates from retail food and humans assigned into similar Multilocus Sequence Types (MLST) were analyzed using whole genome sequencing (WGS). In silico analysis of assembled sequences revealed the existence of multiple resistance genes among the examined E. coli isolates. Of the six CTX-M-producing isolates from retail food, bla (CTX-M-14) was the prevalent variant identified (83.3%, 5/6). Two plasmid-mediated fosfomycin resistance genes, fosA3, and fosA4, were detected from retail food isolates (one each from chicken and beef), where fosA4 was identified in the chicken isolate 82CH that also carried the colistin resistance gene mcr-1. The bla (CTX-M-14) and fosA genes in retail food isolates were located adjacent to insertion sequences ISEcp1 and IS26, respectively. Sequence analysis of the reconstructed mcr-1 plasmid (p82CH) showed 96-97% identity to mcr-1-carrying IncI2 plasmids previously identified in human and food E. coli isolates from Egypt. Hierarchical clustering of core genome MLST (HierCC) revealed clustering of chicken isolate 82CH, co-harboring mcr-1 and fosA4 genes, with a chicken E. coli isolate from China at the HC200 level (",

keywords = "Escherichia coli, cgMLST clustering, fosA, genetic context, mcr-1, retail food, whole genome sequencing",

author = "Hazem Ramadan and Soliman, {Ahmed M.} and Hiott, {Lari M.} and Mohammed Elbediwi and Woodley, {Tiffanie A.} and Chattaway, {Marie A.} and Claire Jenkins and Frye, {Jonathan G.} and Jackson, {Charlene R.}",

note = "Funding Information: This work has been funded by the U.S. Department of Agriculture (USDA) project 6040-32000-079-00-D. The Science, Technology and Innovation Fund Authority (STIFA) of Egypt (Short term fellowship, ID 25449) partially supported this work. The authors acknowledge the Daniel Turnberg UK/Middle East Travel Fellowship for funding the visit of Dr. Hazem Ramadan to Public Health England, UK. Open Access: Copyright {\textcopyright} 2021 Ramadan, Soliman, Hiott, Elbediwi, Woodley, Chattaway, Jenkins, Frye and Jackson. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. Publisher Copyright: {\textcopyright} Copyright {\textcopyright} 2021 Ramadan, Soliman, Hiott, Elbediwi, Woodley, Chattaway, Jenkins, Frye and Jackson. Citation: Ramadan, Hazem, et al. {"}Emergence of Multidrug-Resistant Escherichia coli Producing CTX-M, MCR-1, and FosA in Retail Food From Egypt.{"} Frontiers in cellular and infection microbiology 11 (2021). DOI: https://doi.org/10.3389/fcimb.2021.681588",

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AU - Ramadan, Hazem

AU - Soliman, Ahmed M.

AU - Hiott, Lari M.

AU - Elbediwi, Mohammed

AU - Woodley, Tiffanie A.

AU - Chattaway, Marie A.

AU - Jenkins, Claire

AU - Frye, Jonathan G.

AU - Jackson, Charlene R.

N1 - Funding Information: This work has been funded by the U.S. Department of Agriculture (USDA) project 6040-32000-079-00-D. The Science, Technology and Innovation Fund Authority (STIFA) of Egypt (Short term fellowship, ID 25449) partially supported this work. The authors acknowledge the Daniel Turnberg UK/Middle East Travel Fellowship for funding the visit of Dr. Hazem Ramadan to Public Health England, UK. Open Access: Copyright © 2021 Ramadan, Soliman, Hiott, Elbediwi, Woodley, Chattaway, Jenkins, Frye and Jackson. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. Publisher Copyright: © Copyright © 2021 Ramadan, Soliman, Hiott, Elbediwi, Woodley, Chattaway, Jenkins, Frye and Jackson. Citation: Ramadan, Hazem, et al. "Emergence of Multidrug-Resistant Escherichia coli Producing CTX-M, MCR-1, and FosA in Retail Food From Egypt." Frontiers in cellular and infection microbiology 11 (2021). DOI: https://doi.org/10.3389/fcimb.2021.681588

PY - 2021/7/13

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N2 - In this study, multidrug-resistant (MDR) Escherichia coli isolates from retail food and humans assigned into similar Multilocus Sequence Types (MLST) were analyzed using whole genome sequencing (WGS). In silico analysis of assembled sequences revealed the existence of multiple resistance genes among the examined E. coli isolates. Of the six CTX-M-producing isolates from retail food, bla (CTX-M-14) was the prevalent variant identified (83.3%, 5/6). Two plasmid-mediated fosfomycin resistance genes, fosA3, and fosA4, were detected from retail food isolates (one each from chicken and beef), where fosA4 was identified in the chicken isolate 82CH that also carried the colistin resistance gene mcr-1. The bla (CTX-M-14) and fosA genes in retail food isolates were located adjacent to insertion sequences ISEcp1 and IS26, respectively. Sequence analysis of the reconstructed mcr-1 plasmid (p82CH) showed 96-97% identity to mcr-1-carrying IncI2 plasmids previously identified in human and food E. coli isolates from Egypt. Hierarchical clustering of core genome MLST (HierCC) revealed clustering of chicken isolate 82CH, co-harboring mcr-1 and fosA4 genes, with a chicken E. coli isolate from China at the HC200 level (

AB - In this study, multidrug-resistant (MDR) Escherichia coli isolates from retail food and humans assigned into similar Multilocus Sequence Types (MLST) were analyzed using whole genome sequencing (WGS). In silico analysis of assembled sequences revealed the existence of multiple resistance genes among the examined E. coli isolates. Of the six CTX-M-producing isolates from retail food, bla (CTX-M-14) was the prevalent variant identified (83.3%, 5/6). Two plasmid-mediated fosfomycin resistance genes, fosA3, and fosA4, were detected from retail food isolates (one each from chicken and beef), where fosA4 was identified in the chicken isolate 82CH that also carried the colistin resistance gene mcr-1. The bla (CTX-M-14) and fosA genes in retail food isolates were located adjacent to insertion sequences ISEcp1 and IS26, respectively. Sequence analysis of the reconstructed mcr-1 plasmid (p82CH) showed 96-97% identity to mcr-1-carrying IncI2 plasmids previously identified in human and food E. coli isolates from Egypt. Hierarchical clustering of core genome MLST (HierCC) revealed clustering of chicken isolate 82CH, co-harboring mcr-1 and fosA4 genes, with a chicken E. coli isolate from China at the HC200 level (

KW - Escherichia coli

KW - cgMLST clustering

KW - fosA

KW - genetic context

KW - mcr-1

KW - retail food

KW - whole genome sequencing

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U2 - 10.3389/fcimb.2021.681588

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M3 - Article

AN - SCOPUS:85111580088

SN - 2235-2988

VL - 11

JO - Frontiers in Cellular and Infection Microbiology

JF - Frontiers in Cellular and Infection Microbiology

M1 - 681588

ER -

Emergence of Multidrug-Resistant Escherichia coli Producing CTX-M, MCR-1, and FosA in Retail Food From Egypt

Abstract

Bibliographical note

Keywords

UN SDGs

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