Efficiency of hepatitis A virus removal in six sewage treatment plants from central Tunisia

Imen Ouardani; Carmen F. Manso; Mahjoub Aouni; Jesús L. Romalde

doi:10.1007/s00253-015-6902-9

Efficiency of hepatitis A virus removal in six sewage treatment plants from central Tunisia

Imen Ouardani, Carmen F. Manso, Mahjoub Aouni, Jesús L. Romalde^*

^*Corresponding author for this work

Antiviral Susceptibility Reference Unit General

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

The efficiency of six Tunisian sewage treatment plants (STP) for the removal of hepatitis A virus (HAV) from wastewater was analysed in order to evaluate the potential risk for human health linked to reuse or discharge of treated wastewater into the environment. The STP utilize different biological wastewater treatments including primary treatment, which involves the physical removal of organic and inorganic solids, and secondary treatment that involves different processes, such as activated sludge or lagoon. Quantitative real-time reverse transcription polymerase chain reaction (RT-PCR) and conventional RT-PCR were used for the analysis of the 325 wastewater samples (163 raw and 162 treated) obtained. Results revealed highest contamination in west-central of Tunisia in raw wastewater with 62.96 % of samples positive for HAV and predominance during winter and autumn, whereas east-central region showed 50.62 % of positive samples with high prevalence from winter through summer. The quantitative analysis revealed a range between 4.29 × 10¹ and 1.24 × 10⁵ RNA copies/mL in treated wastewater, showing clearly the inefficiency for total removal of HAV regardless of the treatment method used. The vast majority of HAV sequences belonged to the sub-genotype IA, except one that was assigned to sub-genotype IB.

Original language	English
Pages (from-to)	10759-10769
Number of pages	11
Journal	Applied Microbiology and Biotechnology
Volume	99
Issue number	24
DOIs	https://doi.org/10.1007/s00253-015-6902-9
Publication status	Published - 1 Dec 2015

Bibliographical note

Publisher Copyright:
© 2015, Springer-Verlag Berlin Heidelberg.

Keywords

Genotyping
Hepatitis A
Sewage treatment plants
Wastewater
qRT-PCR

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s00253-015-6902-9

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abstract = "The efficiency of six Tunisian sewage treatment plants (STP) for the removal of hepatitis A virus (HAV) from wastewater was analysed in order to evaluate the potential risk for human health linked to reuse or discharge of treated wastewater into the environment. The STP utilize different biological wastewater treatments including primary treatment, which involves the physical removal of organic and inorganic solids, and secondary treatment that involves different processes, such as activated sludge or lagoon. Quantitative real-time reverse transcription polymerase chain reaction (RT-PCR) and conventional RT-PCR were used for the analysis of the 325 wastewater samples (163 raw and 162 treated) obtained. Results revealed highest contamination in west-central of Tunisia in raw wastewater with 62.96 % of samples positive for HAV and predominance during winter and autumn, whereas east-central region showed 50.62 % of positive samples with high prevalence from winter through summer. The quantitative analysis revealed a range between 4.29 × 101 and 1.24 × 105 RNA copies/mL in treated wastewater, showing clearly the inefficiency for total removal of HAV regardless of the treatment method used. The vast majority of HAV sequences belonged to the sub-genotype IA, except one that was assigned to sub-genotype IB.",

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Efficiency of hepatitis A virus removal in six sewage treatment plants from central Tunisia

Abstract

Bibliographical note

Keywords

UN SDGs

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