Effect of schedule on reactogenicity and antibody persistence of acellular and whole-cell pertussis vaccines: Value of laboratory tests as predictors of clinical performance

Elizbeth Miller*, L. A.E. Ashworth, K. Redhead, C. Thornton, P. A. Waight, T. Coleman

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    53 Citations (Scopus)

    Abstract

    The performance of four acellular pertussis vaccines containing between two and five pertussis antigens combined with diphtheria and tetanus toxoids was compared with that of British whole-cell diphtheria/tetanus/pertussis (DTP) vaccine both in laboratory assays for potency, toxicity and immunogenicity, and for reactogenicity and immunogenicity in infants. Clinical responses were evaluated in double blind randomized Phase II trials using 3/5/9 month and 2/3/4 month schedules. The acellular DTPs had much lower toxicity than whole-cell DTP in laboratory tests and were significantly less pyrogenic than whole-cell DTP under both schedules. Local reactions were not consistently lower in acellular than whole-cell vaccinees and varied with the source of the diphtheria and tetanus antigens used. Differences in endotoxin level and content of active pertussis toxin (PT) between acellular DTP vaccines were not clinically significant. The reactogenicity advantage of the acellular vaccines was substantially reduced under the 2/3/4 month schedule due to the reduced reactogenicity of the whole-cell DTP vaccine when given at a younger age. There was no relationship between antigen content measured in micrograms per dose and ELISA antibody responses to filamentous haemagglutinin (FHA) and PT in infants, nor was murine immunogenicity predictive of immunogenicity in humans. Antibody response to PT was attenuated in the whole-cell group under the 2/3/4 month schedule but was unaffected in the group receiving acellular vaccines with individually purified components; antibody response to pertactin (69 kDa antigen) was similar in recipients of the whole-cell and component acellular vaccines under the 2/3/4 month schedule. PT antibody persistence until 4-5 years of age was significantly better in recipients of the component acellular than either the whole-cell vaccine or the co-purified acellular vaccine under the 3/5/9 month schedule. However, diphtheria antitoxin levels were reduced in acellular vaccine recipients under both schedules. Despite significantly lower tetanus potencies of the acellular vaccines in laboratory tests, no differences were found in tetanus anti-toxin responses in children.

    Original languageEnglish
    Pages (from-to)51-60
    Number of pages10
    JournalVaccine
    Volume15
    Issue number1
    DOIs
    Publication statusPublished - Jan 1997

    Bibliographical note

    Funding Information:
    We thank the generapl ractitionersa nd familiesi n North Hertfordsl~irefo r participatingi n the trials, the study nursesf or their excellentw ork, Joan Vurdien for admin-istratives upporta nd Dr Farrington for his help with the statisticala nalyses.W e also thank Drs Robinson and Irons for the suppfy of PT, FHA and agglutinogen antigensa nd ConnaughtL aboratoriesL td for the gift of the 69 kDa antigen. The trials were conducted with financial support from the Medical Research Council and the Departmento f Health.

    Keywords

    • antibody persistence to acellular and whole-cell pertussis vaccines
    • laboratory performance of acellular and whole-cell pertussis vaccines
    • schedule for acellular and whole-cell pertussis vaccines

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