Effect of monoclonal antibodies on the adherence of Bordetella pertussis to Vero cells

A. R. Gorringe; L. A.E. Ashworth; L. I. Irons; A. Robinson

doi:10.1111/j.1574-6968.1985.tb01556.x

Effect of monoclonal antibodies on the adherence of Bordetella pertussis to Vero cells

A. R. Gorringe^*
, L. A.E. Ashworth
, L. I. Irons
, A. Robinson

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

An in vitro assay involving the adherence of Bordetella pertussis to Vero cells was used to quantify the inhibitory effects of monoclonal antibodies (McAb) on adherence. McAbs to agglutinogens (fimbriae), filamentous haemagglutinin (FHA), lymphocytosis promoting factor (LPF) and to an X-mode specific outer membrane protein were tested. X-mode cells adhered to the Vero cells to a greater extent than C-mode cells. McAb to agglutinogen 2 inhibited the adherence of B. pertussis bearing homologous agglutinogen. McAbs to LPF, FHA, agglutinogen 3 and X-mode specific outer membrane protein also partially inhibited the adherence of B. pertussis to Vero cells.

Original language	English
Pages (from-to)	5-9
Number of pages	5
Journal	FEMS Microbiology Letters
Volume	26
Issue number	1
DOIs	https://doi.org/10.1111/j.1574-6968.1985.tb01556.x https://doi.org/10.1016/0378-1097(85)90115-6
Publication status	Published - Jan 1985

Keywords

Fimbriae
surface antigens

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title = "Effect of monoclonal antibodies on the adherence of Bordetella pertussis to Vero cells",

abstract = "An in vitro assay involving the adherence of Bordetella pertussis to Vero cells was used to quantify the inhibitory effects of monoclonal antibodies (McAb) on adherence. McAbs to agglutinogens (fimbriae), filamentous haemagglutinin (FHA), lymphocytosis promoting factor (LPF) and to an X-mode specific outer membrane protein were tested. X-mode cells adhered to the Vero cells to a greater extent than C-mode cells. McAb to agglutinogen 2 inhibited the adherence of B. pertussis bearing homologous agglutinogen. McAbs to LPF, FHA, agglutinogen 3 and X-mode specific outer membrane protein also partially inhibited the adherence of B. pertussis to Vero cells.",

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AU - Gorringe, A. R.

AU - Ashworth, L. A.E.

AU - Irons, L. I.

AU - Robinson, A.

PY - 1985/1

Y1 - 1985/1

N2 - An in vitro assay involving the adherence of Bordetella pertussis to Vero cells was used to quantify the inhibitory effects of monoclonal antibodies (McAb) on adherence. McAbs to agglutinogens (fimbriae), filamentous haemagglutinin (FHA), lymphocytosis promoting factor (LPF) and to an X-mode specific outer membrane protein were tested. X-mode cells adhered to the Vero cells to a greater extent than C-mode cells. McAb to agglutinogen 2 inhibited the adherence of B. pertussis bearing homologous agglutinogen. McAbs to LPF, FHA, agglutinogen 3 and X-mode specific outer membrane protein also partially inhibited the adherence of B. pertussis to Vero cells.

AB - An in vitro assay involving the adherence of Bordetella pertussis to Vero cells was used to quantify the inhibitory effects of monoclonal antibodies (McAb) on adherence. McAbs to agglutinogens (fimbriae), filamentous haemagglutinin (FHA), lymphocytosis promoting factor (LPF) and to an X-mode specific outer membrane protein were tested. X-mode cells adhered to the Vero cells to a greater extent than C-mode cells. McAb to agglutinogen 2 inhibited the adherence of B. pertussis bearing homologous agglutinogen. McAbs to LPF, FHA, agglutinogen 3 and X-mode specific outer membrane protein also partially inhibited the adherence of B. pertussis to Vero cells.

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KW - surface antigens

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SN - 0378-1097

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ER -

Effect of monoclonal antibodies on the adherence of Bordetella pertussis to Vero cells

Abstract

Keywords

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