DNA-Vaccine-Induced Immune Response Correlates with Lower Viral SARS-CoV-2 Titers in a Ferret Model

Mirco Compagnone; Eleonora Pinto; Erika Salvatori; Lucia Lione; Antonella Conforti; Silvia Marchese; Micol Ravà; Kathryn Ryan; Yper Hall; Emma Rayner; Francisco J. Salguero; Jemma Paterson; Matteo Iannacone; Raffaele De Francesco; Luigi Aurisicchio; Fabio Palombo

doi:10.3390/vaccines10081178

DNA-Vaccine-Induced Immune Response Correlates with Lower Viral SARS-CoV-2 Titers in a Ferret Model

Mirco Compagnone
, Eleonora Pinto
, Erika Salvatori
, Lucia Lione
, Antonella Conforti^*
, Silvia Marchese
, Micol Ravà
, Kathryn Ryan
, Yper Hall
, Emma Rayner
, Francisco J. Salguero
, Jemma Paterson
, Matteo Iannacone
, Raffaele De Francesco
, Luigi Aurisicchio^*
, Fabio Palombo^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

The COVID-19 pandemic is entering a new era with the approval of many SARS-CoV-2 vaccines. In spite of the restoration of an almost normal way of life thanks to the immune protection elicited by these innovative vaccines, we are still facing high viral circulation, with a significant number of deaths. To further explore alternative vaccination platforms, we developed COVID-eVax—a genetic vaccine based on plasmid DNA encoding the RBD domain of the SARS-CoV-2 spike protein. Here, we describe the correlation between immune responses and the evolution of viral infection in ferrets infected with the live virus. We demonstrate COVID-eVax immunogenicity as means of antibody response and, above all, a significant T-cell response, thus proving the critical role of T-cell immunity, in addition to the neutralizing antibody activity, in controlling viral spread.

Original language	English
Article number	1178
Journal	Vaccines
Volume	10
Issue number	8
DOIs	https://doi.org/10.3390/vaccines10081178
Publication status	Published - Aug 2022

Bibliographical note

Publisher Copyright:
© 2022 by the authors.

Keywords

DNA vaccines
SARS-CoV-2
ferrets
immune responses

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.3390/vaccines10081178

Cite this

Compagnone, M., Pinto, E., Salvatori, E., Lione, L., Conforti, A., Marchese, S., Ravà, M., Ryan, K., Hall, Y., Rayner, E., Salguero, F. J., Paterson, J., Iannacone, M., De Francesco, R., Aurisicchio, L., & Palombo, F. (2022). DNA-Vaccine-Induced Immune Response Correlates with Lower Viral SARS-CoV-2 Titers in a Ferret Model. Vaccines, 10(8), Article 1178. https://doi.org/10.3390/vaccines10081178

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title = "DNA-Vaccine-Induced Immune Response Correlates with Lower Viral SARS-CoV-2 Titers in a Ferret Model",

abstract = "The COVID-19 pandemic is entering a new era with the approval of many SARS-CoV-2 vaccines. In spite of the restoration of an almost normal way of life thanks to the immune protection elicited by these innovative vaccines, we are still facing high viral circulation, with a significant number of deaths. To further explore alternative vaccination platforms, we developed COVID-eVax—a genetic vaccine based on plasmid DNA encoding the RBD domain of the SARS-CoV-2 spike protein. Here, we describe the correlation between immune responses and the evolution of viral infection in ferrets infected with the live virus. We demonstrate COVID-eVax immunogenicity as means of antibody response and, above all, a significant T-cell response, thus proving the critical role of T-cell immunity, in addition to the neutralizing antibody activity, in controlling viral spread.",

keywords = "DNA vaccines, SARS-CoV-2, ferrets, immune responses",

author = "Mirco Compagnone and Eleonora Pinto and Erika Salvatori and Lucia Lione and Antonella Conforti and Silvia Marchese and Micol Rav{\`a} and Kathryn Ryan and Yper Hall and Emma Rayner and Salguero, \{Francisco J.\} and Jemma Paterson and Matteo Iannacone and \{De Francesco\}, Raffaele and Luigi Aurisicchio and Fabio Palombo",

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year = "2022",

month = aug,

doi = "10.3390/vaccines10081178",

language = "English",

volume = "10",

journal = "Vaccines",

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Compagnone, M, Pinto, E, Salvatori, E, Lione, L, Conforti, A, Marchese, S, Ravà, M, Ryan, K , Hall, Y, Rayner, E, Salguero, FJ, Paterson, J, Iannacone, M, De Francesco, R, Aurisicchio, L & Palombo, F 2022, 'DNA-Vaccine-Induced Immune Response Correlates with Lower Viral SARS-CoV-2 Titers in a Ferret Model', Vaccines, vol. 10, no. 8, 1178. https://doi.org/10.3390/vaccines10081178

TY - JOUR

T1 - DNA-Vaccine-Induced Immune Response Correlates with Lower Viral SARS-CoV-2 Titers in a Ferret Model

AU - Compagnone, Mirco

AU - Pinto, Eleonora

AU - Salvatori, Erika

AU - Lione, Lucia

AU - Conforti, Antonella

AU - Marchese, Silvia

AU - Ravà, Micol

AU - Ryan, Kathryn

AU - Hall, Yper

AU - Rayner, Emma

AU - Salguero, Francisco J.

AU - Paterson, Jemma

AU - Iannacone, Matteo

AU - De Francesco, Raffaele

AU - Aurisicchio, Luigi

AU - Palombo, Fabio

PY - 2022/8

Y1 - 2022/8

N2 - The COVID-19 pandemic is entering a new era with the approval of many SARS-CoV-2 vaccines. In spite of the restoration of an almost normal way of life thanks to the immune protection elicited by these innovative vaccines, we are still facing high viral circulation, with a significant number of deaths. To further explore alternative vaccination platforms, we developed COVID-eVax—a genetic vaccine based on plasmid DNA encoding the RBD domain of the SARS-CoV-2 spike protein. Here, we describe the correlation between immune responses and the evolution of viral infection in ferrets infected with the live virus. We demonstrate COVID-eVax immunogenicity as means of antibody response and, above all, a significant T-cell response, thus proving the critical role of T-cell immunity, in addition to the neutralizing antibody activity, in controlling viral spread.

AB - The COVID-19 pandemic is entering a new era with the approval of many SARS-CoV-2 vaccines. In spite of the restoration of an almost normal way of life thanks to the immune protection elicited by these innovative vaccines, we are still facing high viral circulation, with a significant number of deaths. To further explore alternative vaccination platforms, we developed COVID-eVax—a genetic vaccine based on plasmid DNA encoding the RBD domain of the SARS-CoV-2 spike protein. Here, we describe the correlation between immune responses and the evolution of viral infection in ferrets infected with the live virus. We demonstrate COVID-eVax immunogenicity as means of antibody response and, above all, a significant T-cell response, thus proving the critical role of T-cell immunity, in addition to the neutralizing antibody activity, in controlling viral spread.

KW - DNA vaccines

KW - SARS-CoV-2

KW - ferrets

KW - immune responses

UR - https://www.scopus.com/pages/publications/85137407148

U2 - 10.3390/vaccines10081178

DO - 10.3390/vaccines10081178

M3 - Article

AN - SCOPUS:85137407148

SN - 2076-393X

VL - 10

JO - Vaccines

JF - Vaccines

IS - 8

M1 - 1178

ER -

DNA-Vaccine-Induced Immune Response Correlates with Lower Viral SARS-CoV-2 Titers in a Ferret Model

Abstract

Bibliographical note

Keywords

UN SDGs

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