Distribution of HPV genotypes in women with cervical cancer in Auckland, New Zealand; A review of 50 specimens between 2000-2006

Deborah Williamson*, Radhika Nagappan, Rao Sirikonda, Fahimeh Rahnama, Stephen Thomas, Margaret Lovell-Smith, Margaret Croxson

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Background: In New Zealand, around two hundred women are diagnosed with cervical cancer annually, with approximately seventy deaths from cervical cancer per year. Aim: Our aim was to determine the distribution of oncogenic HPV genotypes in biopsy specimens from women with diagnosed cervical cancers in the Auckland region of New Zealand between 2000-2006. Materials and methods: Confirmed cases of cervical carcinoma were identified from the local pathology register, and representative tissue samples were taken from these blocks. Sections were deparaffinised, and DNA was extracted according to standard protocols. Samples were subject to PCR amplification using L1 consensus primer sets MY09/11 and GP5/6. Further type-specific amplification was performed on positive samples, using an in-house primer sequence based on target sequences within the E6 gene. Remaining samples were typed by a Linear Array Assay, or by DNA sequencing. Results: HPV DNA was detected in 100% of cases. In 49/50 samples, the HPV genotype was identified, with a total of 14 different HPV genotypes detectable. Together HPV-16 and 18 were found in 41/49 cases (83.6%) either singly or in combination Discussion: Our findings suggest that the distribution of HPV genotypes in New Zealand is similar to that of other geographic areas. Conclusion: Ongoing surveillance is warranted to ensure appropriate genotype selection for prophylactic HPV vaccinations.

Original languageEnglish
Pages (from-to)67-70
Number of pages4
JournalAustralian and New Zealand Journal of Obstetrics and Gynaecology
Volume51
Issue number1
DOIs
Publication statusPublished - Feb 2011
Externally publishedYes

Keywords

  • cervical carcinoma
  • genotyping
  • human papillomavirus

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