Differentiation of non-pylori Helicobacter species based on PCR-restriction fragment length polymorphism of the 23S rRNA gene

Abbas Yadegar; Masoud Alebouyeh; Andy J. Lawson; Tabassom Mirzaei; Ehsan Nazemalhosseini Mojarad; Mohammad Reza Zali

doi:10.1007/s11274-014-1615-2

Differentiation of non-pylori Helicobacter species based on PCR-restriction fragment length polymorphism of the 23S rRNA gene

Abbas Yadegar, Masoud Alebouyeh^*, Andy J. Lawson, Tabassom Mirzaei, Ehsan Nazemalhosseini Mojarad, Mohammad Reza Zali

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

Phenotypic identification of non-pylori Helicobacter species has always been problematic and time-consuming in comparison with many other bacteria. We developed a rapid two-step identification assay based on PCR-restriction fragment length polymorphism (PCR-RFLP) analysis of the 23S rRNA gene for differentiating between non-pylori Helicobacter species. A new genus-specific primer pair based on all available complete and partial 23S rRNA sequences of Helicobacter species was designed. In silico restriction analysis of variable regions of the 23S rRNA gene suggested SmaI and HindIII endonucleases would provide a good level of differentiation. Analysis of the obtained 23S rRNA RFLP patterns divided all Helicobacter study strains into three species groups (groups A-C) and 12 unique restriction patterns. Wolinella succinogenes also gave a unique pattern. Our proposed PCR-RFLP method was found to be as a valuable tool for routine identification of non-pylori Helicobacter species from human or animal samples.

Original language	English
Pages (from-to)	1909-1917
Number of pages	9
Journal	World Journal of Microbiology and Biotechnology
Volume	30
Issue number	6
DOIs	https://doi.org/10.1007/s11274-014-1615-2
Publication status	Published - Jun 2014

Bibliographical note

Funding Information:
Acknowledgments This work was financially supported by a research grant (Code: 574) from Gastroenterology and Liver Diseases Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.

Keywords

23S rRNA
Helicobacter pylori
Non-pyloriHelicobacter species
RFLP
Wolinella succinogenes

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10.1007/s11274-014-1615-2

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title = "Differentiation of non-pylori Helicobacter species based on PCR-restriction fragment length polymorphism of the 23S rRNA gene",

abstract = "Phenotypic identification of non-pylori Helicobacter species has always been problematic and time-consuming in comparison with many other bacteria. We developed a rapid two-step identification assay based on PCR-restriction fragment length polymorphism (PCR-RFLP) analysis of the 23S rRNA gene for differentiating between non-pylori Helicobacter species. A new genus-specific primer pair based on all available complete and partial 23S rRNA sequences of Helicobacter species was designed. In silico restriction analysis of variable regions of the 23S rRNA gene suggested SmaI and HindIII endonucleases would provide a good level of differentiation. Analysis of the obtained 23S rRNA RFLP patterns divided all Helicobacter study strains into three species groups (groups A-C) and 12 unique restriction patterns. Wolinella succinogenes also gave a unique pattern. Our proposed PCR-RFLP method was found to be as a valuable tool for routine identification of non-pylori Helicobacter species from human or animal samples.",

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note = "Funding Information: Acknowledgments This work was financially supported by a research grant (Code: 574) from Gastroenterology and Liver Diseases Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",

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AU - Mirzaei, Tabassom

AU - Mojarad, Ehsan Nazemalhosseini

AU - Zali, Mohammad Reza

N1 - Funding Information: Acknowledgments This work was financially supported by a research grant (Code: 574) from Gastroenterology and Liver Diseases Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.

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AB - Phenotypic identification of non-pylori Helicobacter species has always been problematic and time-consuming in comparison with many other bacteria. We developed a rapid two-step identification assay based on PCR-restriction fragment length polymorphism (PCR-RFLP) analysis of the 23S rRNA gene for differentiating between non-pylori Helicobacter species. A new genus-specific primer pair based on all available complete and partial 23S rRNA sequences of Helicobacter species was designed. In silico restriction analysis of variable regions of the 23S rRNA gene suggested SmaI and HindIII endonucleases would provide a good level of differentiation. Analysis of the obtained 23S rRNA RFLP patterns divided all Helicobacter study strains into three species groups (groups A-C) and 12 unique restriction patterns. Wolinella succinogenes also gave a unique pattern. Our proposed PCR-RFLP method was found to be as a valuable tool for routine identification of non-pylori Helicobacter species from human or animal samples.

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Differentiation of non-pylori Helicobacter species based on PCR-restriction fragment length polymorphism of the 23S rRNA gene

Abstract

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Keywords

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