Development of an unmarked gene deletion system for the filamentous fungi Aspergillus niger and Talaromyces versatilis

Stéphane Delmas, Agustina Llanos, Jean Luc Parrou, Matthew Kokolski, Steven Pullan, Lee Shunburne, David B. Archer*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)

Abstract

In this article, we present a method to delete genes in filamentous fungi that allows recycling of the selection marker and is efficient in a nonhomologous end-joining (NHEJ)-proficient strain. We exemplify the approach by deletion of the gene encoding the transcriptional regulator XlnR in the fungus Aspergillus niger. To show the efficiency and advantages of the method, we deleted 8 other genes and constructed a double mutant in this species. Moreover, we showed that the same principle also functions in a different genus of filamentous fungus (Talaromyces versatilis, basionym Penicillium funiculosum). This technique will increase the versatility of the toolboxes for genome manipulation of model and industrially relevant fungi.

Original languageEnglish
Pages (from-to)3484-3487
Number of pages4
JournalApplied and Environmental Microbiology
Volume80
Issue number11
DOIs
Publication statusPublished - Jun 2014

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