Development of an indirect ELISA method for the parallel measurement of IgG and IgM antibodies against Crimean-Congo haemorrhagic fever (CCHF) virus using recombinant nucleoprotein as antigen

S. D. Dowall; K. S. Richards; V. A. Graham; J. Chamberlain; R. Hewson

doi:10.1016/j.jviromet.2011.11.020

Development of an indirect ELISA method for the parallel measurement of IgG and IgM antibodies against Crimean-Congo haemorrhagic fever (CCHF) virus using recombinant nucleoprotein as antigen

S. D. Dowall^*, K. S. Richards, V. A. Graham, J. Chamberlain, R. Hewson

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

44 Citations (Scopus)

Abstract

Recombinant nucleoprotein from Crimean-Congo Haemorrhagic Fever (CCHF) virus was successfully derived from a baculovirus expression system and purified for use in a novel enzyme-linked immunosorbent assay (ELISA) diagnostic test. Comparable tests were used for detection of IgG and IgM antibodies, thus allowing efficient detection of both antibodies in parallel. The major benefits of the assay also included removing any requirement for polyclonal sera, thus eliminating variation in preparations and allowing standardisation between laboratories. The assay was successfully tested using a panel of positive sera supplied from samples identified as being positive in Turkey, Tajikistan and Kosovo and shown to be sensitive and specific. It is envisaged that this simple diagnostic ELISA for CCHF virus infection which removes the reliance on polyclonal antibody preparations, will be accessible to a wider range of laboratories enabling them to carry out routine diagnosis. This will improve the efficiency of diagnosis and subsequent management of infected patients.

Original language	English
Pages (from-to)	335-341
Number of pages	7
Journal	Journal of Virological Methods
Volume	179
Issue number	2
DOIs	https://doi.org/10.1016/j.jviromet.2011.11.020
Publication status	Published - Feb 2012

Bibliographical note

Funding Information:
We are extremely grateful to the Refik Saydam Hygiene Centre, Turkey (Ahmet Carhan and Yavuz Uyar), the National Institute of Public Health, Kosovo (Salih Ahmeti and Iusuf Dedushaj), the Institute of Preventative Medicine, Dushanbe, Tajikistan (Farida Tishkova) and the University of Helsinki, Finland (Olli Vapalahti) for the provision of sera samples used in this study. We also thank Iain Hay (State University of New York, Buffalo) for his advice on ELISA development. Work was funded by the UK Department of Health. The views expressed in this publication are those of the authors and not necessarily those of the Department of Health.

Keywords

Assay
CCHF
Detection
ELISA
Haemorrhagic
Viruses

Access to Document

10.1016/j.jviromet.2011.11.020

Cite this

Dowall, S. D., Richards, K. S., Graham, V. A., Chamberlain, J., & Hewson, R. (2012). Development of an indirect ELISA method for the parallel measurement of IgG and IgM antibodies against Crimean-Congo haemorrhagic fever (CCHF) virus using recombinant nucleoprotein as antigen. Journal of Virological Methods, 179(2), 335-341. https://doi.org/10.1016/j.jviromet.2011.11.020

@article{14d285dd6e9e4f87adbe158258685e01,

title = "Development of an indirect ELISA method for the parallel measurement of IgG and IgM antibodies against Crimean-Congo haemorrhagic fever (CCHF) virus using recombinant nucleoprotein as antigen",

abstract = "Recombinant nucleoprotein from Crimean-Congo Haemorrhagic Fever (CCHF) virus was successfully derived from a baculovirus expression system and purified for use in a novel enzyme-linked immunosorbent assay (ELISA) diagnostic test. Comparable tests were used for detection of IgG and IgM antibodies, thus allowing efficient detection of both antibodies in parallel. The major benefits of the assay also included removing any requirement for polyclonal sera, thus eliminating variation in preparations and allowing standardisation between laboratories. The assay was successfully tested using a panel of positive sera supplied from samples identified as being positive in Turkey, Tajikistan and Kosovo and shown to be sensitive and specific. It is envisaged that this simple diagnostic ELISA for CCHF virus infection which removes the reliance on polyclonal antibody preparations, will be accessible to a wider range of laboratories enabling them to carry out routine diagnosis. This will improve the efficiency of diagnosis and subsequent management of infected patients.",

keywords = "Assay, CCHF, Detection, ELISA, Haemorrhagic, Viruses",

author = "Dowall, {S. D.} and Richards, {K. S.} and Graham, {V. A.} and J. Chamberlain and R. Hewson",

note = "Funding Information: We are extremely grateful to the Refik Saydam Hygiene Centre, Turkey (Ahmet Carhan and Yavuz Uyar), the National Institute of Public Health, Kosovo (Salih Ahmeti and Iusuf Dedushaj), the Institute of Preventative Medicine, Dushanbe, Tajikistan (Farida Tishkova) and the University of Helsinki, Finland (Olli Vapalahti) for the provision of sera samples used in this study. We also thank Iain Hay (State University of New York, Buffalo) for his advice on ELISA development. Work was funded by the UK Department of Health. The views expressed in this publication are those of the authors and not necessarily those of the Department of Health.",

year = "2012",

month = feb,

doi = "10.1016/j.jviromet.2011.11.020",

language = "English",

volume = "179",

pages = "335--341",

journal = "Journal of Virological Methods",

issn = "0166-0934",

publisher = "Elsevier B.V.",

number = "2",

}

Dowall, SD , Richards, KS, Graham, VA, Chamberlain, J & Hewson, R 2012, 'Development of an indirect ELISA method for the parallel measurement of IgG and IgM antibodies against Crimean-Congo haemorrhagic fever (CCHF) virus using recombinant nucleoprotein as antigen', Journal of Virological Methods, vol. 179, no. 2, pp. 335-341. https://doi.org/10.1016/j.jviromet.2011.11.020

Development of an indirect ELISA method for the parallel measurement of IgG and IgM antibodies against Crimean-Congo haemorrhagic fever (CCHF) virus using recombinant nucleoprotein as antigen. / Dowall, S. D.; Richards, K. S.; Graham, V. A. et al.
In: Journal of Virological Methods, Vol. 179, No. 2, 02.2012, p. 335-341.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Development of an indirect ELISA method for the parallel measurement of IgG and IgM antibodies against Crimean-Congo haemorrhagic fever (CCHF) virus using recombinant nucleoprotein as antigen

AU - Dowall, S. D.

AU - Richards, K. S.

AU - Graham, V. A.

AU - Chamberlain, J.

AU - Hewson, R.

N1 - Funding Information: We are extremely grateful to the Refik Saydam Hygiene Centre, Turkey (Ahmet Carhan and Yavuz Uyar), the National Institute of Public Health, Kosovo (Salih Ahmeti and Iusuf Dedushaj), the Institute of Preventative Medicine, Dushanbe, Tajikistan (Farida Tishkova) and the University of Helsinki, Finland (Olli Vapalahti) for the provision of sera samples used in this study. We also thank Iain Hay (State University of New York, Buffalo) for his advice on ELISA development. Work was funded by the UK Department of Health. The views expressed in this publication are those of the authors and not necessarily those of the Department of Health.

PY - 2012/2

Y1 - 2012/2

N2 - Recombinant nucleoprotein from Crimean-Congo Haemorrhagic Fever (CCHF) virus was successfully derived from a baculovirus expression system and purified for use in a novel enzyme-linked immunosorbent assay (ELISA) diagnostic test. Comparable tests were used for detection of IgG and IgM antibodies, thus allowing efficient detection of both antibodies in parallel. The major benefits of the assay also included removing any requirement for polyclonal sera, thus eliminating variation in preparations and allowing standardisation between laboratories. The assay was successfully tested using a panel of positive sera supplied from samples identified as being positive in Turkey, Tajikistan and Kosovo and shown to be sensitive and specific. It is envisaged that this simple diagnostic ELISA for CCHF virus infection which removes the reliance on polyclonal antibody preparations, will be accessible to a wider range of laboratories enabling them to carry out routine diagnosis. This will improve the efficiency of diagnosis and subsequent management of infected patients.

AB - Recombinant nucleoprotein from Crimean-Congo Haemorrhagic Fever (CCHF) virus was successfully derived from a baculovirus expression system and purified for use in a novel enzyme-linked immunosorbent assay (ELISA) diagnostic test. Comparable tests were used for detection of IgG and IgM antibodies, thus allowing efficient detection of both antibodies in parallel. The major benefits of the assay also included removing any requirement for polyclonal sera, thus eliminating variation in preparations and allowing standardisation between laboratories. The assay was successfully tested using a panel of positive sera supplied from samples identified as being positive in Turkey, Tajikistan and Kosovo and shown to be sensitive and specific. It is envisaged that this simple diagnostic ELISA for CCHF virus infection which removes the reliance on polyclonal antibody preparations, will be accessible to a wider range of laboratories enabling them to carry out routine diagnosis. This will improve the efficiency of diagnosis and subsequent management of infected patients.

KW - Assay

KW - CCHF

KW - Detection

KW - ELISA

KW - Haemorrhagic

KW - Viruses

UR - http://www.scopus.com/inward/record.url?scp=84855479852&partnerID=8YFLogxK

U2 - 10.1016/j.jviromet.2011.11.020

DO - 10.1016/j.jviromet.2011.11.020

M3 - Article

C2 - 22155577

AN - SCOPUS:84855479852

SN - 0166-0934

VL - 179

SP - 335

EP - 341

JO - Journal of Virological Methods

JF - Journal of Virological Methods

IS - 2

ER -

Development of an indirect ELISA method for the parallel measurement of IgG and IgM antibodies against Crimean-Congo haemorrhagic fever (CCHF) virus using recombinant nucleoprotein as antigen

Abstract

Bibliographical note

Keywords

Access to Document

Other files and links

Fingerprint

Cite this