Development of an indirect ELISA method for the parallel measurement of IgG and IgM antibodies against Crimean-Congo haemorrhagic fever (CCHF) virus using recombinant nucleoprotein as antigen

S. D. Dowall*, K. S. Richards, V. A. Graham, J. Chamberlain, R. Hewson

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    40 Citations (Scopus)

    Abstract

    Recombinant nucleoprotein from Crimean-Congo Haemorrhagic Fever (CCHF) virus was successfully derived from a baculovirus expression system and purified for use in a novel enzyme-linked immunosorbent assay (ELISA) diagnostic test. Comparable tests were used for detection of IgG and IgM antibodies, thus allowing efficient detection of both antibodies in parallel. The major benefits of the assay also included removing any requirement for polyclonal sera, thus eliminating variation in preparations and allowing standardisation between laboratories. The assay was successfully tested using a panel of positive sera supplied from samples identified as being positive in Turkey, Tajikistan and Kosovo and shown to be sensitive and specific. It is envisaged that this simple diagnostic ELISA for CCHF virus infection which removes the reliance on polyclonal antibody preparations, will be accessible to a wider range of laboratories enabling them to carry out routine diagnosis. This will improve the efficiency of diagnosis and subsequent management of infected patients.

    Original languageEnglish
    Pages (from-to)335-341
    Number of pages7
    JournalJournal of Virological Methods
    Volume179
    Issue number2
    DOIs
    Publication statusPublished - Feb 2012

    Bibliographical note

    Funding Information:
    We are extremely grateful to the Refik Saydam Hygiene Centre, Turkey (Ahmet Carhan and Yavuz Uyar), the National Institute of Public Health, Kosovo (Salih Ahmeti and Iusuf Dedushaj), the Institute of Preventative Medicine, Dushanbe, Tajikistan (Farida Tishkova) and the University of Helsinki, Finland (Olli Vapalahti) for the provision of sera samples used in this study. We also thank Iain Hay (State University of New York, Buffalo) for his advice on ELISA development. Work was funded by the UK Department of Health. The views expressed in this publication are those of the authors and not necessarily those of the Department of Health.

    Keywords

    • Assay
    • CCHF
    • Detection
    • ELISA
    • Haemorrhagic
    • Viruses

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