Development of a real-time RT-PCR assay for the detection of Crimean-Congo hemorrhagic fever virus

Barry Atkinson; John Chamberlain; Christopher H. Logue; Nicola Cook; Christine Bruce; Stuart Dowall; Roger Hewson

doi:10.1089/vbz.2011.0770

Development of a real-time RT-PCR assay for the detection of Crimean-Congo hemorrhagic fever virus

Barry Atkinson^*, John Chamberlain, Christopher H. Logue, Nicola Cook, Christine Bruce, Stuart Dowall, Roger Hewson

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

98 Citations (Scopus)

Abstract

Crimean-Congo hemorrhagic fever (CCHF) is a virulent tick-borne disease with a case fatality rate ranging from 10-50% for tick-borne transmission, and up to 80% for nosocomial transmission. Human cases have been reported in over 30 countries across Europe, Asia, and Africa. It appears to be spreading to new areas with several countries reporting their first human cases of CCHF disease within the past 10 years. We report a novel real-time RT-PCR assay designed to amplify a conserved region of the CCHF virus S segment. It is capable of detecting strains from all 7 groups of CCHF, including the AP92 strain that until recently represented a lineage of strains that were not associated with human disease. The limit of detection of the assay is 5 copies of target RNA, and the assay shows no cross-reactivity with other viruses from within the same genus, or with viruses causing similar human disease.

Original language	English
Pages (from-to)	786-793
Number of pages	8
Journal	Vector-Borne and Zoonotic Diseases
Volume	12
Issue number	9
DOIs	https://doi.org/10.1089/vbz.2011.0770
Publication status	Published - 1 Sept 2012

Keywords

Assay
CCHF
Crimean-Congo Hemorrhagic Fever
Detection
Real-time RT-PCR

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1089/vbz.2011.0770

Cite this

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title = "Development of a real-time RT-PCR assay for the detection of Crimean-Congo hemorrhagic fever virus",

abstract = "Crimean-Congo hemorrhagic fever (CCHF) is a virulent tick-borne disease with a case fatality rate ranging from 10-50% for tick-borne transmission, and up to 80% for nosocomial transmission. Human cases have been reported in over 30 countries across Europe, Asia, and Africa. It appears to be spreading to new areas with several countries reporting their first human cases of CCHF disease within the past 10 years. We report a novel real-time RT-PCR assay designed to amplify a conserved region of the CCHF virus S segment. It is capable of detecting strains from all 7 groups of CCHF, including the AP92 strain that until recently represented a lineage of strains that were not associated with human disease. The limit of detection of the assay is 5 copies of target RNA, and the assay shows no cross-reactivity with other viruses from within the same genus, or with viruses causing similar human disease.",

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T1 - Development of a real-time RT-PCR assay for the detection of Crimean-Congo hemorrhagic fever virus

AU - Atkinson, Barry

AU - Chamberlain, John

AU - Logue, Christopher H.

AU - Cook, Nicola

AU - Bruce, Christine

AU - Dowall, Stuart

AU - Hewson, Roger

PY - 2012/9/1

Y1 - 2012/9/1

N2 - Crimean-Congo hemorrhagic fever (CCHF) is a virulent tick-borne disease with a case fatality rate ranging from 10-50% for tick-borne transmission, and up to 80% for nosocomial transmission. Human cases have been reported in over 30 countries across Europe, Asia, and Africa. It appears to be spreading to new areas with several countries reporting their first human cases of CCHF disease within the past 10 years. We report a novel real-time RT-PCR assay designed to amplify a conserved region of the CCHF virus S segment. It is capable of detecting strains from all 7 groups of CCHF, including the AP92 strain that until recently represented a lineage of strains that were not associated with human disease. The limit of detection of the assay is 5 copies of target RNA, and the assay shows no cross-reactivity with other viruses from within the same genus, or with viruses causing similar human disease.

AB - Crimean-Congo hemorrhagic fever (CCHF) is a virulent tick-borne disease with a case fatality rate ranging from 10-50% for tick-borne transmission, and up to 80% for nosocomial transmission. Human cases have been reported in over 30 countries across Europe, Asia, and Africa. It appears to be spreading to new areas with several countries reporting their first human cases of CCHF disease within the past 10 years. We report a novel real-time RT-PCR assay designed to amplify a conserved region of the CCHF virus S segment. It is capable of detecting strains from all 7 groups of CCHF, including the AP92 strain that until recently represented a lineage of strains that were not associated with human disease. The limit of detection of the assay is 5 copies of target RNA, and the assay shows no cross-reactivity with other viruses from within the same genus, or with viruses causing similar human disease.

KW - Assay

KW - CCHF

KW - Crimean-Congo Hemorrhagic Fever

KW - Detection

KW - Real-time RT-PCR

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U2 - 10.1089/vbz.2011.0770

DO - 10.1089/vbz.2011.0770

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SN - 1530-3667

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JO - Vector-Borne and Zoonotic Diseases

JF - Vector-Borne and Zoonotic Diseases

IS - 9

ER -

Development of a real-time RT-PCR assay for the detection of Crimean-Congo hemorrhagic fever virus

Abstract

Keywords

UN SDGs

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