Development of a real-time RT-PCR assay for the detection of Crimean-Congo hemorrhagic fever virus

Barry Atkinson*, John Chamberlain, Christopher H. Logue, Nicola Cook, Christine Bruce, Stuart Dowall, Roger Hewson

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    72 Citations (Scopus)

    Abstract

    Crimean-Congo hemorrhagic fever (CCHF) is a virulent tick-borne disease with a case fatality rate ranging from 10-50% for tick-borne transmission, and up to 80% for nosocomial transmission. Human cases have been reported in over 30 countries across Europe, Asia, and Africa. It appears to be spreading to new areas with several countries reporting their first human cases of CCHF disease within the past 10 years. We report a novel real-time RT-PCR assay designed to amplify a conserved region of the CCHF virus S segment. It is capable of detecting strains from all 7 groups of CCHF, including the AP92 strain that until recently represented a lineage of strains that were not associated with human disease. The limit of detection of the assay is 5 copies of target RNA, and the assay shows no cross-reactivity with other viruses from within the same genus, or with viruses causing similar human disease.

    Original languageEnglish
    Pages (from-to)786-793
    Number of pages8
    JournalVector-Borne and Zoonotic Diseases
    Volume12
    Issue number9
    DOIs
    Publication statusPublished - 1 Sep 2012

    Keywords

    • Assay
    • CCHF
    • Crimean-Congo Hemorrhagic Fever
    • Detection
    • Real-time RT-PCR

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