Development of a guinea pig immune response-related microarray and its use to define the host response following Mycobacterium bovis BCG vaccination

Julia A. Tree; Michael J. Elmore; Sajid Javed; Ann Williams; Philip D. Marsh

doi:10.1128/IAI.74.2.1436-1441.2006

Development of a guinea pig immune response-related microarray and its use to define the host response following Mycobacterium bovis BCG vaccination

Julia A. Tree^*, Michael J. Elmore, Sajid Javed, Ann Williams, Philip D. Marsh

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

28 Citations (Scopus)

Abstract

Immune responses in the guinea pig model are understudied because of a lack of commercial reagents. We have developed a custom-made guinea pig oligonucleotide microarray (81 spots) and have examined the gene expression profile of splenocytes restimulated in vitro from Mycobacterium bovis BCG-vaccinated and naive animals. Eleven genes were significantly (P < 0.05) up-regulated following vaccination, indicating a Th1-type response. These results show that microarrays can be used to more fully define immune profiles of guinea pigs.

Original language	English
Pages (from-to)	1436-1441
Number of pages	6
Journal	Infection and Immunity
Volume	74
Issue number	2
DOIs	https://doi.org/10.1128/IAI.74.2.1436-1441.2006
Publication status	Published - Feb 2006

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10.1128/IAI.74.2.1436-1441.2006

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AU - Williams, Ann

AU - Marsh, Philip D.

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AB - Immune responses in the guinea pig model are understudied because of a lack of commercial reagents. We have developed a custom-made guinea pig oligonucleotide microarray (81 spots) and have examined the gene expression profile of splenocytes restimulated in vitro from Mycobacterium bovis BCG-vaccinated and naive animals. Eleven genes were significantly (P < 0.05) up-regulated following vaccination, indicating a Th1-type response. These results show that microarrays can be used to more fully define immune profiles of guinea pigs.

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Development of a guinea pig immune response-related microarray and its use to define the host response following Mycobacterium bovis BCG vaccination

Abstract

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