Development of a guinea pig immune response-related microarray and its use to define the host response following Mycobacterium bovis BCG vaccination

Julia A. Tree*, Michael J. Elmore, Sajid Javed, Ann Williams, Philip D. Marsh

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    28 Citations (Scopus)

    Abstract

    Immune responses in the guinea pig model are understudied because of a lack of commercial reagents. We have developed a custom-made guinea pig oligonucleotide microarray (81 spots) and have examined the gene expression profile of splenocytes restimulated in vitro from Mycobacterium bovis BCG-vaccinated and naive animals. Eleven genes were significantly (P < 0.05) up-regulated following vaccination, indicating a Th1-type response. These results show that microarrays can be used to more fully define immune profiles of guinea pigs.

    Original languageEnglish
    Pages (from-to)1436-1441
    Number of pages6
    JournalInfection and Immunity
    Volume74
    Issue number2
    DOIs
    Publication statusPublished - Feb 2006

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