Abstract
Immune responses in the guinea pig model are understudied because of a lack of commercial reagents. We have developed a custom-made guinea pig oligonucleotide microarray (81 spots) and have examined the gene expression profile of splenocytes restimulated in vitro from Mycobacterium bovis BCG-vaccinated and naive animals. Eleven genes were significantly (P < 0.05) up-regulated following vaccination, indicating a Th1-type response. These results show that microarrays can be used to more fully define immune profiles of guinea pigs.
Original language | English |
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Pages (from-to) | 1436-1441 |
Number of pages | 6 |
Journal | Infection and Immunity |
Volume | 74 |
Issue number | 2 |
DOIs | |
Publication status | Published - Feb 2006 |