Development and evaluation of Varicella zoster virus ELISA for oral fluid suitable for epidemiological studies

Yamima Talukder; Robin Gopal; Nicholas Andrews; Michael Glenn; Judith Breuer; David Brown

doi:10.1016/j.jviromet.2005.04.013

Development and evaluation of Varicella zoster virus ELISA for oral fluid suitable for epidemiological studies

Yamima Talukder^*, Robin Gopal, Nicholas Andrews, Michael Glenn, Judith Breuer, David Brown

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

An enzyme-linked immunosorbent assay (ELISA) for detection of Varicella zoster virus (VZV) antibodies in oral fluid is described. The assay was optimised and evaluated using paired serum and oral fluid from healthy adult volunteers (n = 205) and preschool children (n = 98), oral fluid samples collected for routine measles, mumps and rubella testing (n = 537) and samples from a study of atopic dermatitis (n = 252). As chickenpox is predominantly a childhood disease and most adults are immune, it was crucial to have samples from children aged 1-5 years to evaluate the assay. Mixture modelling of the oral fluid results was used to determine the optimum cut-off, sensitivity and specificity of the assay. Compared to paired sera tested by the same ELISA the sensitivity of the oral fluid assay was 93% and specificity 95.7% overall, varying slightly with age group. The assay was shown to have good potential for use in large-scale epidemiological studies.

Original language	English
Pages (from-to)	162-167
Number of pages	6
Journal	Journal of Virological Methods
Volume	128
Issue number	1-2
DOIs	https://doi.org/10.1016/j.jviromet.2005.04.013
Publication status	Published - Sept 2005

Bibliographical note

Funding Information:
We thank Dr. Bernard Cohen, CPHL for oral fluid samples and helpful advice; Dr. S. Gibbs for oral fluid samples from the atopic dermatitis study; Dr. D. D’Auria, occupational health physician, St. Bartholomew's Hospital for collecting paired samples from student volunteers and new staff for this study; Dr. Elizabeth Miller for permission to use paired sample panels; Yamima Talukder was supported by a PHLS and Medical Research Council collaborative studentship. This work was funded by the Health Protection Agency (formerly PHLS).

Keywords

ELISA
Mixture modelling
Oral fluid
Varicella zoster virus

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.jviromet.2005.04.013

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title = "Development and evaluation of Varicella zoster virus ELISA for oral fluid suitable for epidemiological studies",

abstract = "An enzyme-linked immunosorbent assay (ELISA) for detection of Varicella zoster virus (VZV) antibodies in oral fluid is described. The assay was optimised and evaluated using paired serum and oral fluid from healthy adult volunteers (n = 205) and preschool children (n = 98), oral fluid samples collected for routine measles, mumps and rubella testing (n = 537) and samples from a study of atopic dermatitis (n = 252). As chickenpox is predominantly a childhood disease and most adults are immune, it was crucial to have samples from children aged 1-5 years to evaluate the assay. Mixture modelling of the oral fluid results was used to determine the optimum cut-off, sensitivity and specificity of the assay. Compared to paired sera tested by the same ELISA the sensitivity of the oral fluid assay was 93% and specificity 95.7% overall, varying slightly with age group. The assay was shown to have good potential for use in large-scale epidemiological studies.",

keywords = "ELISA, Mixture modelling, Oral fluid, Varicella zoster virus",

author = "Yamima Talukder and Robin Gopal and Nicholas Andrews and Michael Glenn and Judith Breuer and David Brown",

note = "Funding Information: We thank Dr. Bernard Cohen, CPHL for oral fluid samples and helpful advice; Dr. S. Gibbs for oral fluid samples from the atopic dermatitis study; Dr. D. D{\textquoteright}Auria, occupational health physician, St. Bartholomew's Hospital for collecting paired samples from student volunteers and new staff for this study; Dr. Elizabeth Miller for permission to use paired sample panels; Yamima Talukder was supported by a PHLS and Medical Research Council collaborative studentship. This work was funded by the Health Protection Agency (formerly PHLS). ",

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AU - Andrews, Nicholas

AU - Glenn, Michael

AU - Breuer, Judith

AU - Brown, David

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N2 - An enzyme-linked immunosorbent assay (ELISA) for detection of Varicella zoster virus (VZV) antibodies in oral fluid is described. The assay was optimised and evaluated using paired serum and oral fluid from healthy adult volunteers (n = 205) and preschool children (n = 98), oral fluid samples collected for routine measles, mumps and rubella testing (n = 537) and samples from a study of atopic dermatitis (n = 252). As chickenpox is predominantly a childhood disease and most adults are immune, it was crucial to have samples from children aged 1-5 years to evaluate the assay. Mixture modelling of the oral fluid results was used to determine the optimum cut-off, sensitivity and specificity of the assay. Compared to paired sera tested by the same ELISA the sensitivity of the oral fluid assay was 93% and specificity 95.7% overall, varying slightly with age group. The assay was shown to have good potential for use in large-scale epidemiological studies.

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Development and evaluation of Varicella zoster virus ELISA for oral fluid suitable for epidemiological studies

Abstract

Bibliographical note

Keywords

UN SDGs

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