Development and evaluation of Varicella zoster virus ELISA for oral fluid suitable for epidemiological studies

Yamima Talukder*, Robin Gopal, Nicholas Andrews, Michael Glenn, Judith Breuer, David Brown

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

An enzyme-linked immunosorbent assay (ELISA) for detection of Varicella zoster virus (VZV) antibodies in oral fluid is described. The assay was optimised and evaluated using paired serum and oral fluid from healthy adult volunteers (n = 205) and preschool children (n = 98), oral fluid samples collected for routine measles, mumps and rubella testing (n = 537) and samples from a study of atopic dermatitis (n = 252). As chickenpox is predominantly a childhood disease and most adults are immune, it was crucial to have samples from children aged 1-5 years to evaluate the assay. Mixture modelling of the oral fluid results was used to determine the optimum cut-off, sensitivity and specificity of the assay. Compared to paired sera tested by the same ELISA the sensitivity of the oral fluid assay was 93% and specificity 95.7% overall, varying slightly with age group. The assay was shown to have good potential for use in large-scale epidemiological studies.

Original languageEnglish
Pages (from-to)162-167
Number of pages6
JournalJournal of Virological Methods
Volume128
Issue number1-2
DOIs
Publication statusPublished - Sep 2005

Bibliographical note

Funding Information:
We thank Dr. Bernard Cohen, CPHL for oral fluid samples and helpful advice; Dr. S. Gibbs for oral fluid samples from the atopic dermatitis study; Dr. D. D’Auria, occupational health physician, St. Bartholomew's Hospital for collecting paired samples from student volunteers and new staff for this study; Dr. Elizabeth Miller for permission to use paired sample panels; Yamima Talukder was supported by a PHLS and Medical Research Council collaborative studentship. This work was funded by the Health Protection Agency (formerly PHLS).

Keywords

  • ELISA
  • Mixture modelling
  • Oral fluid
  • Varicella zoster virus

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