Development and evaluation of serological assays for detection of Hantaan virus-specific antibodies in human sera using yeast-expressed nucleocapsid protein

Rasa Petraityte, Hua Yang, Rashpal Hunjan, Aušra Ražanskiene, Pravesh Dhanilall, Rainer G. Ulrich, Kestutis Sasnauskas, Li Jin*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Indirect and capture enzyme-linked immunosorbent assays (ELISAs) for detection of Hantaan virus (HTNV)-specific immunoglobulins G (IgG) and M (IgM) in human serum samples were developed on the basis of recombinant yeast-expressed nucleocapsid (N) protein of HTNV. The sensitivities and specificities of the indirect and capture ELISAs were evaluated by comparing the reactivity of sera from patients with hemorrhagic fever with renal syndrome (HFRS) from China with that of a commercial IgG/IgM kit. The sensitivity of the indirect IgG and IgM ELISA tests was both 100% and the specificity of the indirect IgM and IgG ELISA test was 98% and 99%, respectively. The sensitivity and specificity of the capture IgM ELISA was 100% and 97%, respectively. The novel assays were found to detect HTNV-specific antibodies in acute phase sera from suspected HFRS patients in China. The results indicate that these novel ELISAs are suitable for the diagnosis of HTNV and for sero-epidemiological studies.

Original languageEnglish
Pages (from-to)89-95
Number of pages7
JournalJournal of Virological Methods
Volume148
Issue number1-2
DOIs
Publication statusPublished - Mar 2008

Bibliographical note

Funding Information:
This work was supported by Lithuanian State Science and Studies Foundation (T-83/07). The authors would like to thank David Brown, Bernard Cohen and Andrew Vyse for the helpful discussions (Colindale, HPA, London) and Prof. Zhanqiu Yang and Qing Li (Wuhan University, China) for providing the serum samples.

Keywords

  • Capture ELISA
  • Hantaan virus
  • Indirect ELISA
  • Nucleocapsid protein

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