Determining a unique defining DNA sequence for yeast species using hashing techniques

Jan Jaap Wesselink*, Beatriz De La Iglesia, Stephen A. James, Jo L. Dicks, Ian N. Roberts, Vic J. Rayward-Smith

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)


Motivation: Yeasts are often still identified with physiological growth tests, which are both time consuming and unsuitable for detection of a mixture of organisms. Hence, there is a need for molecular methods to identify yeast species. Results: A hashing technique has been developed to search for unique DNA sequences in 702 26S rRNA genes. A unique DNA sequence has been found for almost every yeast species described to date. The locations of the unique defining sequences are in accordance with the variability map of large subunit ribosomal RNA and provide detail of the evolution of the D1/D2 region. This approach will be applicable to the rapid identification of unique sequences in other DNA sequence sets.

Original languageEnglish
Pages (from-to)1004-1010
Number of pages7
Issue number7
Publication statusPublished - 2002
Externally publishedYes

Bibliographical note

Funding Information:
This work was supported by BBSRC Grant Ref. No. 83/BIO 12037.


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