Detection of rpoB mutations in Mycobacterium tuberculosis by biprobe analysis

K. J. Edwards; L. A. Metherell; M. Yates; N. A. Saunders

doi:10.1128/JCM.39.9.3350-3352.2001

Detection of rpoB mutations in Mycobacterium tuberculosis by biprobe analysis

K. J. Edwards
, L. A. Metherell
, M. Yates
, N. A. Saunders^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

41 Citations (Scopus)

Abstract

A biprobe assay utilizing LightCycler technology was developed to detect rifampin resistance-associated gene mutations in the Mycobacterium tuberculosis rpoB gene. Three biprobes detected all mutations present in the 46 rifampin-resistant isolates. Wild-type sequences were correctly identified in each case. The method was reproducible, accurate, and easy to use.

Original language	English
Pages (from-to)	3350-3352
Number of pages	3
Journal	Journal of Clinical Microbiology
Volume	39
Issue number	9
DOIs	https://doi.org/10.1128/JCM.39.9.3350-3352.2001
Publication status	Published - 2001
Externally published	Yes

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10.1128/JCM.39.9.3350-3352.2001

Cite this

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title = "Detection of rpoB mutations in Mycobacterium tuberculosis by biprobe analysis",

abstract = "A biprobe assay utilizing LightCycler technology was developed to detect rifampin resistance-associated gene mutations in the Mycobacterium tuberculosis rpoB gene. Three biprobes detected all mutations present in the 46 rifampin-resistant isolates. Wild-type sequences were correctly identified in each case. The method was reproducible, accurate, and easy to use.",

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AU - Yates, M.

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AB - A biprobe assay utilizing LightCycler technology was developed to detect rifampin resistance-associated gene mutations in the Mycobacterium tuberculosis rpoB gene. Three biprobes detected all mutations present in the 46 rifampin-resistant isolates. Wild-type sequences were correctly identified in each case. The method was reproducible, accurate, and easy to use.

UR - https://www.scopus.com/pages/publications/0034824244

U2 - 10.1128/JCM.39.9.3350-3352.2001

DO - 10.1128/JCM.39.9.3350-3352.2001

M3 - Article

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AN - SCOPUS:0034824244

SN - 0095-1137

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EP - 3352

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 9

ER -

Detection of rpoB mutations in Mycobacterium tuberculosis by biprobe analysis

Abstract

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