Detection of anti-H5 responses in human sera by HI using horse erythrocytes following MF59-adjuvanted influenza A/Duck/Singapore/97 vaccine

I. Stephenson; J. M. Wood; K. G. Nicholson; Andre Charlett; Maria Zambon

doi:10.1016/j.virusres.2004.02.019

Detection of anti-H5 responses in human sera by HI using horse erythrocytes following MF59-adjuvanted influenza A/Duck/Singapore/97 vaccine

I. Stephenson, J. M. Wood, K. G. Nicholson, Andre Charlett, Maria Zambon^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

169 Citations (Scopus)

Abstract

Haemagglutination-inhibition (HI) tests are a simple method used to assess immune responses to influenza haemagglutinin. However, HI tests are insensitive at detection of antibody responses to avian haemagglutinin after vaccination or natural infection, even in the presence of high titres of neutralising antibody or virus isolation. Avian influenza viruses preferentially bind to sialic acid receptors that contain N-acetylneuraminic acid α2,3-galactose (α2,3Gal) linkages while human viruses preferentially bind to those containing N-acetylneuraminic acid α2,6-galactose (α2,6Gal) linkages. By using horse erythrocytes in the HI test and thereby increasing the proportion of α2,3Gal linkages available for binding, we are able to demonstrate improved detection of antibody to avian H5 in human sera following vaccination with MF59-adjuvanted A/Duck/Singapore/97 surface antigen vaccine. This modified HI test was more sensitive in detection of anti-H5 antibody evoked by revaccination of primed subjects and may be useful in assessing potential avian HA vaccine candidates.

Original language	English
Pages (from-to)	91-95
Number of pages	5
Journal	Virus Research
Volume	103
Issue number	1-2
DOIs	https://doi.org/10.1016/j.virusres.2004.02.019
Publication status	Published - Jul 2004

Keywords

Avian influenza
Haemagglutinin-inhibition
Horse erythrocytes
Receptor specificity

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10.1016/j.virusres.2004.02.019

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title = "Detection of anti-H5 responses in human sera by HI using horse erythrocytes following MF59-adjuvanted influenza A/Duck/Singapore/97 vaccine",

abstract = "Haemagglutination-inhibition (HI) tests are a simple method used to assess immune responses to influenza haemagglutinin. However, HI tests are insensitive at detection of antibody responses to avian haemagglutinin after vaccination or natural infection, even in the presence of high titres of neutralising antibody or virus isolation. Avian influenza viruses preferentially bind to sialic acid receptors that contain N-acetylneuraminic acid α2,3-galactose (α2,3Gal) linkages while human viruses preferentially bind to those containing N-acetylneuraminic acid α2,6-galactose (α2,6Gal) linkages. By using horse erythrocytes in the HI test and thereby increasing the proportion of α2,3Gal linkages available for binding, we are able to demonstrate improved detection of antibody to avian H5 in human sera following vaccination with MF59-adjuvanted A/Duck/Singapore/97 surface antigen vaccine. This modified HI test was more sensitive in detection of anti-H5 antibody evoked by revaccination of primed subjects and may be useful in assessing potential avian HA vaccine candidates.",

keywords = "Avian influenza, Haemagglutinin-inhibition, Horse erythrocytes, Receptor specificity",

author = "I. Stephenson and Wood, {J. M.} and Nicholson, {K. G.} and Andre Charlett and Maria Zambon",

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doi = "10.1016/j.virusres.2004.02.019",

language = "English",

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AU - Stephenson, I.

AU - Wood, J. M.

AU - Nicholson, K. G.

AU - Charlett, Andre

AU - Zambon, Maria

PY - 2004/7

Y1 - 2004/7

N2 - Haemagglutination-inhibition (HI) tests are a simple method used to assess immune responses to influenza haemagglutinin. However, HI tests are insensitive at detection of antibody responses to avian haemagglutinin after vaccination or natural infection, even in the presence of high titres of neutralising antibody or virus isolation. Avian influenza viruses preferentially bind to sialic acid receptors that contain N-acetylneuraminic acid α2,3-galactose (α2,3Gal) linkages while human viruses preferentially bind to those containing N-acetylneuraminic acid α2,6-galactose (α2,6Gal) linkages. By using horse erythrocytes in the HI test and thereby increasing the proportion of α2,3Gal linkages available for binding, we are able to demonstrate improved detection of antibody to avian H5 in human sera following vaccination with MF59-adjuvanted A/Duck/Singapore/97 surface antigen vaccine. This modified HI test was more sensitive in detection of anti-H5 antibody evoked by revaccination of primed subjects and may be useful in assessing potential avian HA vaccine candidates.

AB - Haemagglutination-inhibition (HI) tests are a simple method used to assess immune responses to influenza haemagglutinin. However, HI tests are insensitive at detection of antibody responses to avian haemagglutinin after vaccination or natural infection, even in the presence of high titres of neutralising antibody or virus isolation. Avian influenza viruses preferentially bind to sialic acid receptors that contain N-acetylneuraminic acid α2,3-galactose (α2,3Gal) linkages while human viruses preferentially bind to those containing N-acetylneuraminic acid α2,6-galactose (α2,6Gal) linkages. By using horse erythrocytes in the HI test and thereby increasing the proportion of α2,3Gal linkages available for binding, we are able to demonstrate improved detection of antibody to avian H5 in human sera following vaccination with MF59-adjuvanted A/Duck/Singapore/97 surface antigen vaccine. This modified HI test was more sensitive in detection of anti-H5 antibody evoked by revaccination of primed subjects and may be useful in assessing potential avian HA vaccine candidates.

KW - Avian influenza

KW - Haemagglutinin-inhibition

KW - Horse erythrocytes

KW - Receptor specificity

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Detection of anti-H5 responses in human sera by HI using horse erythrocytes following MF59-adjuvanted influenza A/Duck/Singapore/97 vaccine

Abstract

Keywords

UN SDGs

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