Detection of a novel human coronavirus by real-time reverse-transcription polymerase chain reaction

V. M. Corman; I. Eckerle; T. Bleicker; A. Zaki; O. Landt; M. Eschbach-Bludau; S. van Boheemen; R. Gopal; M. Ballhause; T. M. Bestebroer; D. Muth; M. A. Müller; J. F. Drexler; M. Zambon; A. D. Osterhaus; R. M. Fouchier; C. Drosten

doi:10.2807/ese.17.39.20285-en

Detection of a novel human coronavirus by real-time reverse-transcription polymerase chain reaction

V. M. Corman, I. Eckerle, T. Bleicker, A. Zaki, O. Landt, M. Eschbach-Bludau, S. van Boheemen, R. Gopal, M. Ballhause, T. M. Bestebroer, D. Muth, M. A. Müller, J. F. Drexler, M. Zambon, A. D. Osterhaus, R. M. Fouchier, C. Drosten^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

461 Citations (Scopus)

Abstract

We present two real-time reverse-transcription polymerase chain reaction assays for a novel human coronavirus (CoV), targeting regions upstream of the E gene (upE) or within open reading frame (ORF)1b, respectively. Sensitivity for upE is 3.4 copies per reaction (95% confidence interval (CI): 2.5-6.9 copies) or 291 copies/mL of sample. No cross-reactivity was observed with coronaviruses OC43, NL63, 229E, SARS-CoV, nor with 92 clinical specimens containing common human respiratory viruses. We recommend using upE for screening and ORF1b for confirmation.

Original language	English
Journal	Eurosurveillance
Volume	17
Issue number	39
DOIs	https://doi.org/10.2807/ese.17.39.20285-en
Publication status	Published - Sept 2012

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.2807/ese.17.39.20285-en

Cite this

Corman, V. M., Eckerle, I., Bleicker, T., Zaki, A., Landt, O., Eschbach-Bludau, M., van Boheemen, S., Gopal, R., Ballhause, M., Bestebroer, T. M., Muth, D., Müller, M. A., Drexler, J. F., Zambon, M., Osterhaus, A. D., Fouchier, R. M., & Drosten, C. (2012). Detection of a novel human coronavirus by real-time reverse-transcription polymerase chain reaction. Eurosurveillance, 17(39). https://doi.org/10.2807/ese.17.39.20285-en

@article{93c21679ebfa4ce587edc54f85956067,

title = "Detection of a novel human coronavirus by real-time reverse-transcription polymerase chain reaction",

abstract = "We present two real-time reverse-transcription polymerase chain reaction assays for a novel human coronavirus (CoV), targeting regions upstream of the E gene (upE) or within open reading frame (ORF)1b, respectively. Sensitivity for upE is 3.4 copies per reaction (95\% confidence interval (CI): 2.5-6.9 copies) or 291 copies/mL of sample. No cross-reactivity was observed with coronaviruses OC43, NL63, 229E, SARS-CoV, nor with 92 clinical specimens containing common human respiratory viruses. We recommend using upE for screening and ORF1b for confirmation.",

author = "Corman, \{V. M.\} and I. Eckerle and T. Bleicker and A. Zaki and O. Landt and M. Eschbach-Bludau and \{van Boheemen\}, S. and R. Gopal and M. Ballhause and Bestebroer, \{T. M.\} and D. Muth and M{\"u}ller, \{M. A.\} and Drexler, \{J. F.\} and M. Zambon and Osterhaus, \{A. D.\} and Fouchier, \{R. M.\} and C. Drosten",

year = "2012",

month = sep,

doi = "10.2807/ese.17.39.20285-en",

language = "English",

volume = "17",

journal = "Eurosurveillance",

issn = "1025-496X",

publisher = "European Centre for Disease Prevention and Control (ECDC)",

number = "39",

}

Corman, VM, Eckerle, I, Bleicker, T, Zaki, A, Landt, O, Eschbach-Bludau, M, van Boheemen, S, Gopal, R, Ballhause, M, Bestebroer, TM, Muth, D, Müller, MA, Drexler, JF, Zambon, M, Osterhaus, AD, Fouchier, RM & Drosten, C 2012, 'Detection of a novel human coronavirus by real-time reverse-transcription polymerase chain reaction', Eurosurveillance, vol. 17, no. 39. https://doi.org/10.2807/ese.17.39.20285-en

TY - JOUR

T1 - Detection of a novel human coronavirus by real-time reverse-transcription polymerase chain reaction

AU - Corman, V. M.

AU - Eckerle, I.

AU - Bleicker, T.

AU - Zaki, A.

AU - Landt, O.

AU - Eschbach-Bludau, M.

AU - van Boheemen, S.

AU - Gopal, R.

AU - Ballhause, M.

AU - Bestebroer, T. M.

AU - Muth, D.

AU - Müller, M. A.

AU - Drexler, J. F.

AU - Zambon, M.

AU - Osterhaus, A. D.

AU - Fouchier, R. M.

AU - Drosten, C.

PY - 2012/9

Y1 - 2012/9

N2 - We present two real-time reverse-transcription polymerase chain reaction assays for a novel human coronavirus (CoV), targeting regions upstream of the E gene (upE) or within open reading frame (ORF)1b, respectively. Sensitivity for upE is 3.4 copies per reaction (95% confidence interval (CI): 2.5-6.9 copies) or 291 copies/mL of sample. No cross-reactivity was observed with coronaviruses OC43, NL63, 229E, SARS-CoV, nor with 92 clinical specimens containing common human respiratory viruses. We recommend using upE for screening and ORF1b for confirmation.

AB - We present two real-time reverse-transcription polymerase chain reaction assays for a novel human coronavirus (CoV), targeting regions upstream of the E gene (upE) or within open reading frame (ORF)1b, respectively. Sensitivity for upE is 3.4 copies per reaction (95% confidence interval (CI): 2.5-6.9 copies) or 291 copies/mL of sample. No cross-reactivity was observed with coronaviruses OC43, NL63, 229E, SARS-CoV, nor with 92 clinical specimens containing common human respiratory viruses. We recommend using upE for screening and ORF1b for confirmation.

UR - https://www.scopus.com/pages/publications/84867136420

U2 - 10.2807/ese.17.39.20285-en

DO - 10.2807/ese.17.39.20285-en

M3 - Article

C2 - 23041020

AN - SCOPUS:84867136420

SN - 1025-496X

VL - 17

JO - Eurosurveillance

JF - Eurosurveillance

IS - 39

ER -

Detection of a novel human coronavirus by real-time reverse-transcription polymerase chain reaction

Abstract

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this