Detection of a novel human coronavirus by real-time reverse-transcription polymerase chain reaction

V. M. Corman; I. Eckerle; T. Bleicker; A. Zaki; O. Landt; M. Eschbach-Bludau; S. van Boheemen; R. Gopal; M. Ballhause; T. M. Bestebroer; D. Muth; M. A. Müller; J. F. Drexler; M. Zambon; A. D. Osterhaus; R. M. Fouchier; C. Drosten

doi:10.2807/ese.17.39.20285-en

Detection of a novel human coronavirus by real-time reverse-transcription polymerase chain reaction

V. M. Corman, I. Eckerle, T. Bleicker, A. Zaki, O. Landt, M. Eschbach-Bludau, S. van Boheemen, R. Gopal, M. Ballhause, T. M. Bestebroer, D. Muth, M. A. Müller, J. F. Drexler, M. Zambon, A. D. Osterhaus, R. M. Fouchier, C. Drosten^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

448 Citations (Scopus)

Abstract

We present two real-time reverse-transcription polymerase chain reaction assays for a novel human coronavirus (CoV), targeting regions upstream of the E gene (upE) or within open reading frame (ORF)1b, respectively. Sensitivity for upE is 3.4 copies per reaction (95% confidence interval (CI): 2.5-6.9 copies) or 291 copies/mL of sample. No cross-reactivity was observed with coronaviruses OC43, NL63, 229E, SARS-CoV, nor with 92 clinical specimens containing common human respiratory viruses. We recommend using upE for screening and ORF1b for confirmation.

Original language	English
Journal	Eurosurveillance
Volume	17
Issue number	39
DOIs	https://doi.org/10.2807/ese.17.39.20285-en
Publication status	Published - Sept 2012

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Corman, V. M., Eckerle, I., Bleicker, T., Zaki, A., Landt, O., Eschbach-Bludau, M., van Boheemen, S., Gopal, R., Ballhause, M., Bestebroer, T. M., Muth, D., Müller, M. A., Drexler, J. F., Zambon, M., Osterhaus, A. D., Fouchier, R. M., & Drosten, C. (2012). Detection of a novel human coronavirus by real-time reverse-transcription polymerase chain reaction. Eurosurveillance, 17(39). https://doi.org/10.2807/ese.17.39.20285-en

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title = "Detection of a novel human coronavirus by real-time reverse-transcription polymerase chain reaction",

abstract = "We present two real-time reverse-transcription polymerase chain reaction assays for a novel human coronavirus (CoV), targeting regions upstream of the E gene (upE) or within open reading frame (ORF)1b, respectively. Sensitivity for upE is 3.4 copies per reaction (95% confidence interval (CI): 2.5-6.9 copies) or 291 copies/mL of sample. No cross-reactivity was observed with coronaviruses OC43, NL63, 229E, SARS-CoV, nor with 92 clinical specimens containing common human respiratory viruses. We recommend using upE for screening and ORF1b for confirmation.",

author = "Corman, {V. M.} and I. Eckerle and T. Bleicker and A. Zaki and O. Landt and M. Eschbach-Bludau and {van Boheemen}, S. and R. Gopal and M. Ballhause and Bestebroer, {T. M.} and D. Muth and M{\"u}ller, {M. A.} and Drexler, {J. F.} and M. Zambon and Osterhaus, {A. D.} and Fouchier, {R. M.} and C. Drosten",

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Corman, VM, Eckerle, I, Bleicker, T, Zaki, A, Landt, O, Eschbach-Bludau, M, van Boheemen, S, Gopal, R, Ballhause, M, Bestebroer, TM, Muth, D, Müller, MA, Drexler, JF, Zambon, M, Osterhaus, AD, Fouchier, RM & Drosten, C 2012, 'Detection of a novel human coronavirus by real-time reverse-transcription polymerase chain reaction', Eurosurveillance, vol. 17, no. 39. https://doi.org/10.2807/ese.17.39.20285-en

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AU - Corman, V. M.

AU - Eckerle, I.

AU - Bleicker, T.

AU - Zaki, A.

AU - Landt, O.

AU - Eschbach-Bludau, M.

AU - van Boheemen, S.

AU - Gopal, R.

AU - Ballhause, M.

AU - Bestebroer, T. M.

AU - Muth, D.

AU - Müller, M. A.

AU - Drexler, J. F.

AU - Zambon, M.

AU - Osterhaus, A. D.

AU - Fouchier, R. M.

AU - Drosten, C.

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Detection of a novel human coronavirus by real-time reverse-transcription polymerase chain reaction

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