Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR

Victor M. Corman; Olfert Landt; Marco Kaiser; Richard Molenkamp; Adam Meijer; Daniel K.W. Chu; Tobias Bleicker; Sebastian Brünink; Julia Schneider; Marie Luisa Schmidt; Daphne G.J.C. Mulders; Bart L. Haagmans; Bas Van Der Veer; Sharon Van Den Brink; Lisa Wijsman; Gabriel Goderski; Jean Louis Romette; Joanna Ellis; Maria Zambon; Malik Peiris; Herman Goossens; Chantal Reusken; Marion P.G. Koopmans; Christian Drosten

doi:10.2807/1560-7917.ES.2020.25.3.2000045

Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR

Victor M. Corman
, Olfert Landt
, Marco Kaiser
, Richard Molenkamp
, Adam Meijer
, Daniel K.W. Chu
, Tobias Bleicker
, Sebastian Brünink
, Julia Schneider
, Marie Luisa Schmidt
, Daphne G.J.C. Mulders
, Bart L. Haagmans
, Bas Van Der Veer
, Sharon Van Den Brink
, Lisa Wijsman
, Gabriel Goderski
, Jean Louis Romette
, Joanna Ellis
, Maria Zambon
, Malik Peiris

Herman Goossens, Chantal Reusken, Marion P.G. Koopmans, Christian Drosten^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

5636 Citations (Scopus)

55 Downloads (Pure)

Abstract

Background: The ongoing outbreak of the recently emerged novel coronavirus (2019-nCoV) poses a challenge for public health laboratories as virus isolates are unavailable while there is growing evidence that the outbreak is more widespread than initially thought, and international spread through travellers does already occur. Aim: We aimed to develop and deploy robust diagnostic methodology for use in public health laboratory settings without having virus material available.

Methods: Here we present a validated diagnostic workflow for 2019-nCoV, its design relying on close genetic relatedness of 2019-nCoV with SARS coronavirus, making use of synthetic nucleic acid technology.

Results: The workflow reliably detects 2019-nCoV, and further discriminates 2019-nCoV from SARS-CoV. Through coordination between academic and public laboratories, we confirmed assay exclusivity based on 297 original clinical specimens containing a full spectrum of human respiratory viruses. Control material is made available through European Virus Archive Global (EVAg), a European Union infrastructure project.

Conclusion: The present study demonstrates the enormous response capacity achieved through coordination of academic and public laboratories in national and European research networks.

Original language	English
Article number	2000045
Journal	Eurosurveillance
Volume	25
Issue number	3
DOIs	https://doi.org/10.2807/1560-7917.ES.2020.25.3.2000045
Publication status	Published - 23 Jan 2020

Bibliographical note

Funding Information: This work was funded by European Union DG Research through projects Prepare (GA602525), Compare (GA643476), and EVAg (GA653316); by European Union DG SANCO through EVD-LabNet, as well as by the German Ministry of Research through projects RAPID (01KI1723A) and DZIF (301-4-7-01.703).

Olfert Landt is CEO of Tib-Molbiol; Marco Kaiser is senior researcher at GenExpress and serves as scientific advisor for
Tib-Molbiol.

Open Access: This is an open-access article distributed under the terms of the Creative Commons Attribution (CC BY 4.0) Licence. You may share and adapt the material, but must give appropriate
credit to the source, provide a link to the licence and indicate
if changes were made.

Publisher Copyright: This article is copyright of the authors or their affiliated institution.

Citation: Corman Victor M, Landt Olfert, Kaiser Marco, Molenkamp Richard, Meijer Adam, Chu Daniel KW, Bleicker Tobias, Brünink Sebastian, Schneider Julia, Schmidt Marie Luisa, Mulders Daphne GJC, Haagmans Bart L, van der Veer Bas, van den Brink Sharon, Wijsman Lisa, Goderski Gabriel, Romette Jean-Louis, Ellis Joanna, Zambon Maria, Peiris Malik, Goossens Herman, Reusken Chantal, Koopmans Marion PG, Drosten Christian. Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR. Euro Surveill. 2020;25(3):pii=2000045.

DOI: https://doi.org/10.2807/1560-7917.ES.2020.25.3.2000045

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Cite this

Corman, V. M., Landt, O., Kaiser, M., Molenkamp, R., Meijer, A., Chu, D. K. W., Bleicker, T., Brünink, S., Schneider, J., Schmidt, M. L., Mulders, D. G. J. C., Haagmans, B. L., Van Der Veer, B., Van Den Brink, S., Wijsman, L., Goderski, G., Romette, J. L., Ellis, J., Zambon, M., ... Drosten, C. (2020). Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR. Eurosurveillance, 25(3), Article 2000045. https://doi.org/10.2807/1560-7917.ES.2020.25.3.2000045

@article{817665d1e7884b2d96da5228034727af,

title = "Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR",

abstract = "Background: The ongoing outbreak of the recently emerged novel coronavirus (2019-nCoV) poses a challenge for public health laboratories as virus isolates are unavailable while there is growing evidence that the outbreak is more widespread than initially thought, and international spread through travellers does already occur. Aim: We aimed to develop and deploy robust diagnostic methodology for use in public health laboratory settings without having virus material available. Methods: Here we present a validated diagnostic workflow for 2019-nCoV, its design relying on close genetic relatedness of 2019-nCoV with SARS coronavirus, making use of synthetic nucleic acid technology. Results: The workflow reliably detects 2019-nCoV, and further discriminates 2019-nCoV from SARS-CoV. Through coordination between academic and public laboratories, we confirmed assay exclusivity based on 297 original clinical specimens containing a full spectrum of human respiratory viruses. Control material is made available through European Virus Archive Global (EVAg), a European Union infrastructure project. Conclusion: The present study demonstrates the enormous response capacity achieved through coordination of academic and public laboratories in national and European research networks.",

author = "Corman, \{Victor M.\} and Olfert Landt and Marco Kaiser and Richard Molenkamp and Adam Meijer and Chu, \{Daniel K.W.\} and Tobias Bleicker and Sebastian Br{\"u}nink and Julia Schneider and Schmidt, \{Marie Luisa\} and Mulders, \{Daphne G.J.C.\} and Haagmans, \{Bart L.\} and \{Van Der Veer\}, Bas and \{Van Den Brink\}, Sharon and Lisa Wijsman and Gabriel Goderski and Romette, \{Jean Louis\} and Joanna Ellis and Maria Zambon and Malik Peiris and Herman Goossens and Chantal Reusken and Koopmans, \{Marion P.G.\} and Christian Drosten",

note = "Funding Information: This work was funded by European Union DG Research through projects Prepare (GA602525), Compare (GA643476), and EVAg (GA653316); by European Union DG SANCO through EVD-LabNet, as well as by the German Ministry of Research through projects RAPID (01KI1723A) and DZIF (301-4-7-01.703). Olfert Landt is CEO of Tib-Molbiol; Marco Kaiser is senior researcher at GenExpress and serves as scientific advisor for Tib-Molbiol. Open Access: This is an open-access article distributed under the terms of the Creative Commons Attribution (CC BY 4.0) Licence. You may share and adapt the material, but must give appropriate credit to the source, provide a link to the licence and indicate if changes were made. Publisher Copyright: This article is copyright of the authors or their affiliated institution. Citation: Corman Victor M, Landt Olfert, Kaiser Marco, Molenkamp Richard, Meijer Adam, Chu Daniel KW, Bleicker Tobias, Br{\"u}nink Sebastian, Schneider Julia, Schmidt Marie Luisa, Mulders Daphne GJC, Haagmans Bart L, van der Veer Bas, van den Brink Sharon, Wijsman Lisa, Goderski Gabriel, Romette Jean-Louis, Ellis Joanna, Zambon Maria, Peiris Malik, Goossens Herman, Reusken Chantal, Koopmans Marion PG, Drosten Christian. Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR. Euro Surveill. 2020;25(3):pii=2000045. DOI: https://doi.org/10.2807/1560-7917.ES.2020.25.3.2000045",

year = "2020",

month = jan,

day = "23",

doi = "10.2807/1560-7917.ES.2020.25.3.2000045",

language = "English",

volume = "25",

journal = "Eurosurveillance",

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Corman, VM, Landt, O, Kaiser, M, Molenkamp, R, Meijer, A, Chu, DKW, Bleicker, T, Brünink, S, Schneider, J, Schmidt, ML, Mulders, DGJC, Haagmans, BL, Van Der Veer, B, Van Den Brink, S, Wijsman, L, Goderski, G, Romette, JL, Ellis, J, Zambon, M, Peiris, M, Goossens, H, Reusken, C, Koopmans, MPG & Drosten, C 2020, 'Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR', Eurosurveillance, vol. 25, no. 3, 2000045. https://doi.org/10.2807/1560-7917.ES.2020.25.3.2000045

TY - JOUR

T1 - Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR

AU - Corman, Victor M.

AU - Landt, Olfert

AU - Kaiser, Marco

AU - Molenkamp, Richard

AU - Meijer, Adam

AU - Chu, Daniel K.W.

AU - Bleicker, Tobias

AU - Brünink, Sebastian

AU - Schneider, Julia

AU - Schmidt, Marie Luisa

AU - Mulders, Daphne G.J.C.

AU - Haagmans, Bart L.

AU - Van Der Veer, Bas

AU - Van Den Brink, Sharon

AU - Wijsman, Lisa

AU - Goderski, Gabriel

AU - Romette, Jean Louis

AU - Ellis, Joanna

AU - Zambon, Maria

AU - Peiris, Malik

AU - Goossens, Herman

AU - Reusken, Chantal

AU - Koopmans, Marion P.G.

AU - Drosten, Christian

N1 - Funding Information: This work was funded by European Union DG Research through projects Prepare (GA602525), Compare (GA643476), and EVAg (GA653316); by European Union DG SANCO through EVD-LabNet, as well as by the German Ministry of Research through projects RAPID (01KI1723A) and DZIF (301-4-7-01.703). Olfert Landt is CEO of Tib-Molbiol; Marco Kaiser is senior researcher at GenExpress and serves as scientific advisor for Tib-Molbiol. Open Access: This is an open-access article distributed under the terms of the Creative Commons Attribution (CC BY 4.0) Licence. You may share and adapt the material, but must give appropriate credit to the source, provide a link to the licence and indicate if changes were made. Publisher Copyright: This article is copyright of the authors or their affiliated institution. Citation: Corman Victor M, Landt Olfert, Kaiser Marco, Molenkamp Richard, Meijer Adam, Chu Daniel KW, Bleicker Tobias, Brünink Sebastian, Schneider Julia, Schmidt Marie Luisa, Mulders Daphne GJC, Haagmans Bart L, van der Veer Bas, van den Brink Sharon, Wijsman Lisa, Goderski Gabriel, Romette Jean-Louis, Ellis Joanna, Zambon Maria, Peiris Malik, Goossens Herman, Reusken Chantal, Koopmans Marion PG, Drosten Christian. Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR. Euro Surveill. 2020;25(3):pii=2000045. DOI: https://doi.org/10.2807/1560-7917.ES.2020.25.3.2000045

PY - 2020/1/23

Y1 - 2020/1/23

N2 - Background: The ongoing outbreak of the recently emerged novel coronavirus (2019-nCoV) poses a challenge for public health laboratories as virus isolates are unavailable while there is growing evidence that the outbreak is more widespread than initially thought, and international spread through travellers does already occur. Aim: We aimed to develop and deploy robust diagnostic methodology for use in public health laboratory settings without having virus material available. Methods: Here we present a validated diagnostic workflow for 2019-nCoV, its design relying on close genetic relatedness of 2019-nCoV with SARS coronavirus, making use of synthetic nucleic acid technology. Results: The workflow reliably detects 2019-nCoV, and further discriminates 2019-nCoV from SARS-CoV. Through coordination between academic and public laboratories, we confirmed assay exclusivity based on 297 original clinical specimens containing a full spectrum of human respiratory viruses. Control material is made available through European Virus Archive Global (EVAg), a European Union infrastructure project. Conclusion: The present study demonstrates the enormous response capacity achieved through coordination of academic and public laboratories in national and European research networks.

AB - Background: The ongoing outbreak of the recently emerged novel coronavirus (2019-nCoV) poses a challenge for public health laboratories as virus isolates are unavailable while there is growing evidence that the outbreak is more widespread than initially thought, and international spread through travellers does already occur. Aim: We aimed to develop and deploy robust diagnostic methodology for use in public health laboratory settings without having virus material available. Methods: Here we present a validated diagnostic workflow for 2019-nCoV, its design relying on close genetic relatedness of 2019-nCoV with SARS coronavirus, making use of synthetic nucleic acid technology. Results: The workflow reliably detects 2019-nCoV, and further discriminates 2019-nCoV from SARS-CoV. Through coordination between academic and public laboratories, we confirmed assay exclusivity based on 297 original clinical specimens containing a full spectrum of human respiratory viruses. Control material is made available through European Virus Archive Global (EVAg), a European Union infrastructure project. Conclusion: The present study demonstrates the enormous response capacity achieved through coordination of academic and public laboratories in national and European research networks.

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U2 - 10.2807/1560-7917.ES.2020.25.3.2000045

DO - 10.2807/1560-7917.ES.2020.25.3.2000045

M3 - Article

C2 - 31992387

AN - SCOPUS:85078687750

SN - 1025-496X

VL - 25

JO - Eurosurveillance

JF - Eurosurveillance

IS - 3

M1 - 2000045

ER -

Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR

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