Delayed chromosomal instability in lymphocytes of cancer patients after radiotherapy

Volodymyr A. Vinnikov; Nataliya A. Maznyk; David Lloyd

doi:10.3109/09553000903564026

Delayed chromosomal instability in lymphocytes of cancer patients after radiotherapy

Volodymyr A. Vinnikov, Nataliya A. Maznyk, David Lloyd

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

Purpose:To assess possible delayed chromosomal instability (DCI) expressed as elevated chromatid breakage in cells containing previously formed chromosome type aberrations in cultured blood lymphocytes of cancer patients after radiotherapy (RT). Materials and methods:Twenty patients treated for uterine cancer with external Co⁶⁰ RT, without chemotherapy, were selected. Blood was taken before, 12 days after RT and one year later. Lymphocytes were cultured for 50 and 100h. Metaphases were stained with fluorescence-plus-Giemsa and analysed for chromosome and chromatid aberrations in 1st (M1) and 3rd plus later (M3) mitoses. Results:RT caused a significant increase of radiation-specific chromosome aberrations in patients' lymphocytes together with DCI, which was observed as an excessive yield of cells containing both chromosome and chromatid aberrations (defined as Cacs&act). This DCI passed successfully through mitoses invitro, and at the end of RT a mean yield of 'extra' Cacs&act was 3×10^-3×cell^-1 amongst either M1 or M3 cells. At the end of RT and one year later DCI in M1 lymphocytes appeared at random amongst patients, but some inter-individual variation was found for DCI presence in M3 cells at both post-irradiation samplings. As time passed, the mean yield of lymphocytes exhibiting DCI decreased invivo and one year after RT reached the pre-treatment level of 1×10^-3×cell^-1. Conclusions:DCI was demonstrated in descendants of human lymphocytes after therapeutic irradiation. The effect diminished one year later, suggesting that the progeny of patients' irradiated stem cells did not produce new daughter lymphocytes exhibiting DCI during the studied post-irradiation period.

Original language	English
Pages (from-to)	271-282
Number of pages	12
Journal	International Journal of Radiation Biology
Volume	86
Issue number	4
DOIs	https://doi.org/10.3109/09553000903564026
Publication status	Published - Apr 2010

Bibliographical note

Funding Information:
The work was carried out as a part of two projects (AMN 02.05 and AMN 02.08) of the Academy of Medical Science of Ukraine. V.A.V. was supported by a Young Scientist Fellowship from the International Association for the promotion of cooperation with scientists from the New Independent States of the former Soviet Union (INTAS YS 05-109-46-43). The authors would like to thank Dr Elizabeth Ainsbury of the UK Health Protection Agency for the discussion of statistical issues in this research.

Keywords

Bystander effects
Chromatid aberrations
Chromosomal instability
Chromosome aberrations
Radiotherapy
Uterine cancer

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.3109/09553000903564026

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@article{b82727599b7b464b8b56049ed2887afe,

title = "Delayed chromosomal instability in lymphocytes of cancer patients after radiotherapy",

abstract = "Purpose:To assess possible delayed chromosomal instability (DCI) expressed as elevated chromatid breakage in cells containing previously formed chromosome type aberrations in cultured blood lymphocytes of cancer patients after radiotherapy (RT). Materials and methods:Twenty patients treated for uterine cancer with external Co60 RT, without chemotherapy, were selected. Blood was taken before, 12 days after RT and one year later. Lymphocytes were cultured for 50 and 100h. Metaphases were stained with fluorescence-plus-Giemsa and analysed for chromosome and chromatid aberrations in 1st (M1) and 3rd plus later (M3) mitoses. Results:RT caused a significant increase of radiation-specific chromosome aberrations in patients' lymphocytes together with DCI, which was observed as an excessive yield of cells containing both chromosome and chromatid aberrations (defined as Cacs&act). This DCI passed successfully through mitoses invitro, and at the end of RT a mean yield of 'extra' Cacs&act was 3×10-3×cell-1 amongst either M1 or M3 cells. At the end of RT and one year later DCI in M1 lymphocytes appeared at random amongst patients, but some inter-individual variation was found for DCI presence in M3 cells at both post-irradiation samplings. As time passed, the mean yield of lymphocytes exhibiting DCI decreased invivo and one year after RT reached the pre-treatment level of 1×10-3×cell-1. Conclusions:DCI was demonstrated in descendants of human lymphocytes after therapeutic irradiation. The effect diminished one year later, suggesting that the progeny of patients' irradiated stem cells did not produce new daughter lymphocytes exhibiting DCI during the studied post-irradiation period.",

keywords = "Bystander effects, Chromatid aberrations, Chromosomal instability, Chromosome aberrations, Radiotherapy, Uterine cancer",

author = "Vinnikov, {Volodymyr A.} and Maznyk, {Nataliya A.} and David Lloyd",

note = "Funding Information: The work was carried out as a part of two projects (AMN 02.05 and AMN 02.08) of the Academy of Medical Science of Ukraine. V.A.V. was supported by a Young Scientist Fellowship from the International Association for the promotion of cooperation with scientists from the New Independent States of the former Soviet Union (INTAS YS 05-109-46-43). The authors would like to thank Dr Elizabeth Ainsbury of the UK Health Protection Agency for the discussion of statistical issues in this research.",

year = "2010",

month = apr,

doi = "10.3109/09553000903564026",

language = "English",

volume = "86",

pages = "271--282",

journal = "International Journal of Radiation Biology",

issn = "0955-3002",

publisher = "Taylor and Francis",

number = "4",

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TY - JOUR

T1 - Delayed chromosomal instability in lymphocytes of cancer patients after radiotherapy

AU - Vinnikov, Volodymyr A.

AU - Maznyk, Nataliya A.

AU - Lloyd, David

N1 - Funding Information: The work was carried out as a part of two projects (AMN 02.05 and AMN 02.08) of the Academy of Medical Science of Ukraine. V.A.V. was supported by a Young Scientist Fellowship from the International Association for the promotion of cooperation with scientists from the New Independent States of the former Soviet Union (INTAS YS 05-109-46-43). The authors would like to thank Dr Elizabeth Ainsbury of the UK Health Protection Agency for the discussion of statistical issues in this research.

PY - 2010/4

Y1 - 2010/4

N2 - Purpose:To assess possible delayed chromosomal instability (DCI) expressed as elevated chromatid breakage in cells containing previously formed chromosome type aberrations in cultured blood lymphocytes of cancer patients after radiotherapy (RT). Materials and methods:Twenty patients treated for uterine cancer with external Co60 RT, without chemotherapy, were selected. Blood was taken before, 12 days after RT and one year later. Lymphocytes were cultured for 50 and 100h. Metaphases were stained with fluorescence-plus-Giemsa and analysed for chromosome and chromatid aberrations in 1st (M1) and 3rd plus later (M3) mitoses. Results:RT caused a significant increase of radiation-specific chromosome aberrations in patients' lymphocytes together with DCI, which was observed as an excessive yield of cells containing both chromosome and chromatid aberrations (defined as Cacs&act). This DCI passed successfully through mitoses invitro, and at the end of RT a mean yield of 'extra' Cacs&act was 3×10-3×cell-1 amongst either M1 or M3 cells. At the end of RT and one year later DCI in M1 lymphocytes appeared at random amongst patients, but some inter-individual variation was found for DCI presence in M3 cells at both post-irradiation samplings. As time passed, the mean yield of lymphocytes exhibiting DCI decreased invivo and one year after RT reached the pre-treatment level of 1×10-3×cell-1. Conclusions:DCI was demonstrated in descendants of human lymphocytes after therapeutic irradiation. The effect diminished one year later, suggesting that the progeny of patients' irradiated stem cells did not produce new daughter lymphocytes exhibiting DCI during the studied post-irradiation period.

AB - Purpose:To assess possible delayed chromosomal instability (DCI) expressed as elevated chromatid breakage in cells containing previously formed chromosome type aberrations in cultured blood lymphocytes of cancer patients after radiotherapy (RT). Materials and methods:Twenty patients treated for uterine cancer with external Co60 RT, without chemotherapy, were selected. Blood was taken before, 12 days after RT and one year later. Lymphocytes were cultured for 50 and 100h. Metaphases were stained with fluorescence-plus-Giemsa and analysed for chromosome and chromatid aberrations in 1st (M1) and 3rd plus later (M3) mitoses. Results:RT caused a significant increase of radiation-specific chromosome aberrations in patients' lymphocytes together with DCI, which was observed as an excessive yield of cells containing both chromosome and chromatid aberrations (defined as Cacs&act). This DCI passed successfully through mitoses invitro, and at the end of RT a mean yield of 'extra' Cacs&act was 3×10-3×cell-1 amongst either M1 or M3 cells. At the end of RT and one year later DCI in M1 lymphocytes appeared at random amongst patients, but some inter-individual variation was found for DCI presence in M3 cells at both post-irradiation samplings. As time passed, the mean yield of lymphocytes exhibiting DCI decreased invivo and one year after RT reached the pre-treatment level of 1×10-3×cell-1. Conclusions:DCI was demonstrated in descendants of human lymphocytes after therapeutic irradiation. The effect diminished one year later, suggesting that the progeny of patients' irradiated stem cells did not produce new daughter lymphocytes exhibiting DCI during the studied post-irradiation period.

KW - Bystander effects

KW - Chromatid aberrations

KW - Chromosomal instability

KW - Chromosome aberrations

KW - Radiotherapy

KW - Uterine cancer

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U2 - 10.3109/09553000903564026

DO - 10.3109/09553000903564026

M3 - Article

C2 - 20353337

AN - SCOPUS:77950513160

SN - 0955-3002

VL - 86

SP - 271

EP - 282

JO - International Journal of Radiation Biology

JF - International Journal of Radiation Biology

IS - 4

ER -

Delayed chromosomal instability in lymphocytes of cancer patients after radiotherapy

Abstract

Bibliographical note

Keywords

UN SDGs

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