Culture of Helicobacter pylori from domestic water samples - The impact of strain variation on growth on solid and in liquid media

Robert J. Owen*, Stephanie Chisholm, G. Brick, J. V. Lee, S. Surman-Lee, S. Lai, B. Said, Gordon Nichols

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

6 Citations (Scopus)

Abstract

Helicobacter pylori is an important global human pathogen and there is growing evidence from PCR assays that contaminated drinking water might be a possible source of infection in some circumstances. There are no validated protocols for direct isolation but various culture media have been developed for possible environmental sampling. Our aim here was to investigate how inter-strain variation might affect the interpretation of results with such media. Two laboratory adapted reference strains and four recent clinical isolates were tested on four solid media and in ten liquid media. Considerable variation was found between strains in their ability to recover on the different media after stress exposure (suspension in sterile tap water). Generally, clinical isolates were less robust than the laboratory-adapted strains and, overall, the former required longer recovery times. Our findings highlighted the importance of using a range of isolates for evaluations, as examination of laboratory-adapted strains alone did not provide an accurate representation of the utility of media that may be used to recover H. pylori from water.

Original languageEnglish
Title of host publicationWater Science and Technology
EditorsJoan Rose, Gertjan G.
Pages147-152
Number of pages6
Edition3
DOIs
Publication statusPublished - 2006

Publication series

NameWater Science and Technology
Number3
Volume54
ISSN (Print)0273-1223

Keywords

  • Culture media
  • Helicobacter pylori
  • Strain diversity
  • Tap water

Fingerprint

Dive into the research topics of 'Culture of Helicobacter pylori from domestic water samples - The impact of strain variation on growth on solid and in liquid media'. Together they form a unique fingerprint.

Cite this