Confirmation of specificity of reactivity in a solid phase ELISA for the detection of hepatitis E viral antigen improves utility of the assay

M. J. Ankcorn; Samreen Ijaz; B. Haywood; J. Neuberger; A. M. Elsharkawy; J. Maggs; Richard Tedder

doi:10.1016/j.jviromet.2017.11.007

Confirmation of specificity of reactivity in a solid phase ELISA for the detection of hepatitis E viral antigen improves utility of the assay

M. J. Ankcorn^*, Samreen Ijaz, B. Haywood, J. Neuberger, A. M. Elsharkawy, J. Maggs, Richard Tedder

^*Corresponding author for this work

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Abstract

Genotype 3 hepatitis E virus (HEV) can lead to persistent infections in immunocompromised hosts. A recently available commercial assay for the detection of HEV antigen (HEV-Ag ELISA, Wantai diagnostics) may enable the study of HEV-Ag dynamics in such persistent infections, however currently there is no confirmatory test available. We generated a putative neutralising reagent from a pool of four convalescent blood donor samples and explored neutralising activity against HEV antigens from clinical samples, HEV tissue-culture and virus-like particles. Using this neutralisation method we were able to differentiate true reactivity from non-specific reactivity in plasma, stool and urine samples. This could also facilitate the introduction of HEV-Ag detection as a screening assay or the study of HEV-Ag in different body fluids.

Original language	English
Pages (from-to)	42-48
Number of pages	7
Journal	Journal of Virological Methods
Volume	252
DOIs	https://doi.org/10.1016/j.jviromet.2017.11.007
Publication status	Published - Feb 2018

Bibliographical note

Funding Information:
The study was funded by NHS Blood and Transplant (NHSBT) through support of Dr Ankcorn’s MD (Res). The funder had no input into study design, data collection, interpretation of the data, the writing of the report, nor the decision to submit for publication.

Publisher Copyright:
© 2017 Elsevier B.V.

Keywords

Antigens
Chronic infection
Hepatitis E virus
Neutralisation

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.jviromet.2017.11.007

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abstract = "Genotype 3 hepatitis E virus (HEV) can lead to persistent infections in immunocompromised hosts. A recently available commercial assay for the detection of HEV antigen (HEV-Ag ELISA, Wantai diagnostics) may enable the study of HEV-Ag dynamics in such persistent infections, however currently there is no confirmatory test available. We generated a putative neutralising reagent from a pool of four convalescent blood donor samples and explored neutralising activity against HEV antigens from clinical samples, HEV tissue-culture and virus-like particles. Using this neutralisation method we were able to differentiate true reactivity from non-specific reactivity in plasma, stool and urine samples. This could also facilitate the introduction of HEV-Ag detection as a screening assay or the study of HEV-Ag in different body fluids.",

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author = "Ankcorn, {M. J.} and Samreen Ijaz and B. Haywood and J. Neuberger and Elsharkawy, {A. M.} and J. Maggs and Richard Tedder",

note = "Funding Information: The study was funded by NHS Blood and Transplant (NHSBT) through support of Dr Ankcorn{\textquoteright}s MD (Res). The funder had no input into study design, data collection, interpretation of the data, the writing of the report, nor the decision to submit for publication. Publisher Copyright: {\textcopyright} 2017 Elsevier B.V.",

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doi = "10.1016/j.jviromet.2017.11.007",

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AU - Ijaz, Samreen

AU - Haywood, B.

AU - Neuberger, J.

AU - Elsharkawy, A. M.

AU - Maggs, J.

AU - Tedder, Richard

N1 - Funding Information: The study was funded by NHS Blood and Transplant (NHSBT) through support of Dr Ankcorn’s MD (Res). The funder had no input into study design, data collection, interpretation of the data, the writing of the report, nor the decision to submit for publication. Publisher Copyright: © 2017 Elsevier B.V.

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N2 - Genotype 3 hepatitis E virus (HEV) can lead to persistent infections in immunocompromised hosts. A recently available commercial assay for the detection of HEV antigen (HEV-Ag ELISA, Wantai diagnostics) may enable the study of HEV-Ag dynamics in such persistent infections, however currently there is no confirmatory test available. We generated a putative neutralising reagent from a pool of four convalescent blood donor samples and explored neutralising activity against HEV antigens from clinical samples, HEV tissue-culture and virus-like particles. Using this neutralisation method we were able to differentiate true reactivity from non-specific reactivity in plasma, stool and urine samples. This could also facilitate the introduction of HEV-Ag detection as a screening assay or the study of HEV-Ag in different body fluids.

AB - Genotype 3 hepatitis E virus (HEV) can lead to persistent infections in immunocompromised hosts. A recently available commercial assay for the detection of HEV antigen (HEV-Ag ELISA, Wantai diagnostics) may enable the study of HEV-Ag dynamics in such persistent infections, however currently there is no confirmatory test available. We generated a putative neutralising reagent from a pool of four convalescent blood donor samples and explored neutralising activity against HEV antigens from clinical samples, HEV tissue-culture and virus-like particles. Using this neutralisation method we were able to differentiate true reactivity from non-specific reactivity in plasma, stool and urine samples. This could also facilitate the introduction of HEV-Ag detection as a screening assay or the study of HEV-Ag in different body fluids.

KW - Antigens

KW - Chronic infection

KW - Hepatitis E virus

KW - Neutralisation

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SN - 0166-0934

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JO - Journal of Virological Methods

JF - Journal of Virological Methods

ER -

Confirmation of specificity of reactivity in a solid phase ELISA for the detection of hepatitis E viral antigen improves utility of the assay

Abstract

Bibliographical note

Keywords

UN SDGs

Access to Document

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