Comparison of two chromogenic media for the detection of vancomycin-resistant enterococcal carriage by nursing home residents

Theodore Gouliouris; Beth Blane; Hayley J. Brodrick; Kathy E. Raven; Kirsty E. Ambridge; Angela D. Kidney; Nazreen F. Hadjirin; M. Estée Török; Direk Limmathurotsakul; Sharon Peacock

doi:10.1016/j.diagmicrobio.2016.04.026

Comparison of two chromogenic media for the detection of vancomycin-resistant enterococcal carriage by nursing home residents

Theodore Gouliouris^*
, Beth Blane
, Hayley J. Brodrick
, Kathy E. Raven
, Kirsty E. Ambridge
, Angela D. Kidney
, Nazreen F. Hadjirin
, M. Estée Török
, Direk Limmathurotsakul
, Sharon Peacock

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

We compared ChromID VRE and Brilliance VRE media for the detection of vancomycin-resistant enterococci (VRE). Using a panel of 28 enterococcal isolates, 10 vanA Enterococcus faecium and three vanA Enterococcus faecalis isolates grew as per manufacturers’ instructions whilst growth of two vanC and eight vancomycin-susceptible enterococci was inhibited on both media. Important differences were noted in the selectivity and chromogenic properties of the two media for vanA Enterococcus raffinosus and vanB E. faecium. The two media were further evaluated using 295 stool samples from nursing home residents, 34 of which grew VRE (11.5%). ChromID and Brilliance had comparable sensitivity, which was increased markedly by prolonging incubation to 48 hours (from 29% to 82%, and from 41% to 85%, respectively) and by a pre-enrichment step (to 97% and 100%, respectively). Brilliance VRE agar had higher selectivity at 48 hours, and after pre-enrichment.

Original language	English
Pages (from-to)	409-412
Number of pages	4
Journal	Diagnostic Microbiology and Infectious Disease
Volume	85
Issue number	4
DOIs	https://doi.org/10.1016/j.diagmicrobio.2016.04.026
Publication status	Published - 1 Aug 2016

Bibliographical note

Publisher Copyright:
© 2016 The Authors

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Detection
Enterococcus faecium
Selectivity
Sensitivity
VRE
Vancomycin-resistant enterococci

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10.1016/j.diagmicrobio.2016.04.026

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Gouliouris, T., Blane, B., Brodrick, H. J., Raven, K. E., Ambridge, K. E., Kidney, A. D., Hadjirin, N. F., Török, M. E., Limmathurotsakul, D., & Peacock, S. (2016). Comparison of two chromogenic media for the detection of vancomycin-resistant enterococcal carriage by nursing home residents. Diagnostic Microbiology and Infectious Disease, 85(4), 409-412. https://doi.org/10.1016/j.diagmicrobio.2016.04.026

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title = "Comparison of two chromogenic media for the detection of vancomycin-resistant enterococcal carriage by nursing home residents",

abstract = "We compared ChromID VRE and Brilliance VRE media for the detection of vancomycin-resistant enterococci (VRE). Using a panel of 28 enterococcal isolates, 10 vanA Enterococcus faecium and three vanA Enterococcus faecalis isolates grew as per manufacturers{\textquoteright} instructions whilst growth of two vanC and eight vancomycin-susceptible enterococci was inhibited on both media. Important differences were noted in the selectivity and chromogenic properties of the two media for vanA Enterococcus raffinosus and vanB E. faecium. The two media were further evaluated using 295 stool samples from nursing home residents, 34 of which grew VRE (11.5\%). ChromID and Brilliance had comparable sensitivity, which was increased markedly by prolonging incubation to 48 hours (from 29\% to 82\%, and from 41\% to 85\%, respectively) and by a pre-enrichment step (to 97\% and 100\%, respectively). Brilliance VRE agar had higher selectivity at 48 hours, and after pre-enrichment.",

keywords = "Detection, Enterococcus faecium, Selectivity, Sensitivity, VRE, Vancomycin-resistant enterococci",

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doi = "10.1016/j.diagmicrobio.2016.04.026",

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Gouliouris, T, Blane, B, Brodrick, HJ, Raven, KE, Ambridge, KE, Kidney, AD, Hadjirin, NF, Török, ME, Limmathurotsakul, D & Peacock, S 2016, 'Comparison of two chromogenic media for the detection of vancomycin-resistant enterococcal carriage by nursing home residents', Diagnostic Microbiology and Infectious Disease, vol. 85, no. 4, pp. 409-412. https://doi.org/10.1016/j.diagmicrobio.2016.04.026

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T1 - Comparison of two chromogenic media for the detection of vancomycin-resistant enterococcal carriage by nursing home residents

AU - Gouliouris, Theodore

AU - Blane, Beth

AU - Brodrick, Hayley J.

AU - Raven, Kathy E.

AU - Ambridge, Kirsty E.

AU - Kidney, Angela D.

AU - Hadjirin, Nazreen F.

AU - Török, M. Estée

AU - Limmathurotsakul, Direk

AU - Peacock, Sharon

PY - 2016/8/1

Y1 - 2016/8/1

N2 - We compared ChromID VRE and Brilliance VRE media for the detection of vancomycin-resistant enterococci (VRE). Using a panel of 28 enterococcal isolates, 10 vanA Enterococcus faecium and three vanA Enterococcus faecalis isolates grew as per manufacturers’ instructions whilst growth of two vanC and eight vancomycin-susceptible enterococci was inhibited on both media. Important differences were noted in the selectivity and chromogenic properties of the two media for vanA Enterococcus raffinosus and vanB E. faecium. The two media were further evaluated using 295 stool samples from nursing home residents, 34 of which grew VRE (11.5%). ChromID and Brilliance had comparable sensitivity, which was increased markedly by prolonging incubation to 48 hours (from 29% to 82%, and from 41% to 85%, respectively) and by a pre-enrichment step (to 97% and 100%, respectively). Brilliance VRE agar had higher selectivity at 48 hours, and after pre-enrichment.

AB - We compared ChromID VRE and Brilliance VRE media for the detection of vancomycin-resistant enterococci (VRE). Using a panel of 28 enterococcal isolates, 10 vanA Enterococcus faecium and three vanA Enterococcus faecalis isolates grew as per manufacturers’ instructions whilst growth of two vanC and eight vancomycin-susceptible enterococci was inhibited on both media. Important differences were noted in the selectivity and chromogenic properties of the two media for vanA Enterococcus raffinosus and vanB E. faecium. The two media were further evaluated using 295 stool samples from nursing home residents, 34 of which grew VRE (11.5%). ChromID and Brilliance had comparable sensitivity, which was increased markedly by prolonging incubation to 48 hours (from 29% to 82%, and from 41% to 85%, respectively) and by a pre-enrichment step (to 97% and 100%, respectively). Brilliance VRE agar had higher selectivity at 48 hours, and after pre-enrichment.

KW - Detection

KW - Enterococcus faecium

KW - Selectivity

KW - Sensitivity

KW - VRE

KW - Vancomycin-resistant enterococci

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VL - 85

SP - 409

EP - 412

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JF - Diagnostic Microbiology and Infectious Disease

IS - 4

ER -

Comparison of two chromogenic media for the detection of vancomycin-resistant enterococcal carriage by nursing home residents

Abstract

Bibliographical note

UN SDGs

Keywords

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